Product Details
| Alternative Name: | Pentraxin 3, TSG-14, TNF stimulated gene-14 |
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| Host: | Rabbit |
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| Immunogen: | Recombinant human PTX3. |
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| UniProt ID: | P26022 |
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| Species reactivity: | Human, Mouse, Rat
Chicken
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| Specificity: | Recognizes human, mouse and rat PTX3 and chicken homolog PPTX. |
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| Crossreactivity: | Weakly cross-reacts with bovine PTX3. |
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| Applications: | ELISA, ICC, IHC (FS), IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | ELISA (detection, 1:20,000)
Western Blot (500ng/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Excellent for use as detection antibody in an ELISA format together with mAb to PTX3 (Prod. No. ALX-804-464 (capture)) and PTX3 standard (Prod. No. ALX-201-124). |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.1% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 2: Pulmonary PTX3 expression by immunohistochemistry (IHC) using PTX3, pAb (Prod. No. ALX-210-365). Method: Photomicrograph of PTX3 stained lung tissue of Cigarette Smoke (CS)-exposed WT mice. Mice were exposed to CS for 4 weeks (subacute).(V: vein, A: artery, AW: airway. Magnification 200×). In courtesy of Guy Brusselle.
Figure:Lysates of normal lung fibroblasts (WI38) and of human fibrosarcoma (8387) were probed with the PTX3, pAb, (Prod. No. ALX-210-365), over night at +4 °C at a concentration of 500ng/ml in 5% dry milk in PBS+/+ containing 0.05% Tween 20. The signal was revealed with an HRP-conjugated anti-rat IgG secondary antibody in 5% dry milk PBS+/+ containing 0.05% Tween 20 after incubation for 1 hour at room temperature and IMMOBILON Western Chemiluminescent substrate from Millipore.
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Product Literature References
Early expression of PTX3 in Aspergillus fumigatus infected rat cornea: J. Zhang, et al.; Int. J. Ophthalmol.
11, 1084 (2018),
Abstract;
Full Text
Pentraxin-3 Is a TSH-Inducible Protein in Human Fibrocytes and Orbital Fibroblasts: H. Wang, et al.; Endocrinology
156, 4336 (2015),
Application(s): Western blot of primary fibroblast lysates,
Abstract;
Full Text
Cigarette smoke induces PTX3 expression in pulmonary veins of mice in an IL-1 dependent manner: N.S. Pauwels, et al.; Respir. Res.
11, 134 (2010),
Abstract;
The Relationship Between Serum Pentraxin 3 and Central Obesity in ST-Segment Elevation Myocardial Infarction Patients: B.J. Shim, et al.; Korean Circ. J.
40, 308 (2010),
Abstract;
Full Text
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant.
22, 2224 (2007),
Abstract;
Elevated maternal levels of the long pentraxin 3 (PTX3) in preeclampsia and intrauterine growth restriction: I. Cetin, et al.; Am. J. Obstet. Gynecol.
194, 1374 (2006),
Abstract;
Full Text
A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel: D.E. Duzhyy, et al.; J. Biol. Chem.
280, 15165 (2005),
Abstract;
The long pentraxin Ptx3 is synthesized in IgA glomerulonephritis and activates mesangial cells: B. Bussolati, et al.; J. Immunol.
170, 1466 (2003),
Abstract;
PTX3, A prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans: G. Peri, et al.; Circulation
102, 636 (2000),
Abstract;
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Expression of a long pentraxin, PTX3, by monocytes exposed to the mycobacterial cell wall component lipoarabinomannan: V. Vouret-Craviari; Infect. Immun.
65, 1345 (1997),
Abstract;
Inducible expression of PTX3, a new member of the pentraxin family, in human mononuclear phagocytes: V.V. Alles, et al.; Blood
84, 3483 (1994),
Abstract;
Full Text
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