Product Details
| Alternative Name: | ERβ |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 100-115 (A100EPQKSPWCEARSLEH115) of human, mouse and rat ERβ (estrogen receptor β). |
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| UniProt ID: | Q92731 |
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| Species reactivity: | Human, Mouse, Rat
Monkey, Sheep
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| Crossreactivity: | Does not cross-react with ERα. |
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| Applications: | ICC, IHC (FS), IHC (PS), WB
Gel Shift
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| Recommended Dilutions/Conditions: | Gel Shift Assay (1-5µg/ml)
Immunocytochemistry (2-4µg/ml)
Immunohistochemistry (paraffin sections, 1-2µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~55kDa by Western blot. |
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| Purity Detail: | Epitope-affinity purified IgG. |
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| Formulation: | Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 2: IHC of ER β in primate amygdala using Estrogen receptor β, pAb (Prod. No. ALX-210-135).
Figure 1: Estrogen receptor β, pAb (Prod. No. ALX-210-135) used in immunofluorescence.
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Product Literature References
Transcription and translation of estrogen receptor b in the male reproductive tract of estrogen receptor a-knock-out and wild-type mice: C.S. Rosenfeld, et al.; Endocrinology
139, 2982 (1998),
Abstract;
A new actor in the estrogen receptor drama--enter ER-beta: B.S. Katzenellenbogen & K.S. Korach; Endocrinology
138, 861 (1997), (Editorial),
Abstract;
Cloning, chromosomal localization, and functional analysis of the murine estrogen receptor beta: G.B. Tremblay, et al.; Mol. Endocrinol.
11, 353 (1997),
Abstract;
Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta: G.G.J.H. Kuiper, et al.; Endocrinology
138, 863 (1997),
Abstract;
Differing roles found for estrogen's two receptors: E. Pennisi; Science
277, 1439 (1997), (Research News),
Abstract;
Distribution of estrogen receptor-beta-like immunoreactivity in rat forebrain: X. Li, et al.; Neuroendocrinology
66, 63 (1997),
Abstract;
Cloning of a novel receptor expressed in rat prostate and ovary: G.G.J.H. Kuiper, et al.; PNAS
93, 5925 (1996),
Abstract;
ER beta: identification and characterization of a novel human estrogen receptor: S. Mosselman, et al.; FEBS Lett.
392, 49 (1996),
Abstract;
