Product Details
| Alternative Name: | Lysosomal-associated membrane protein 1 |
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| Clone: | LY1C6 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Rat liver lysosomal membrane preparation. |
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| UniProt ID: | P14562 |
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| GenBank ID: | M34959 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Rat
Hamster
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| Applications: | ICC, IF, IP, WB
Electron microscopy
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~120kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Lysosome-associated membrane proteins (LAMP1 and LAMP2) are type I integral membrane protein that are transported from the trans-Golgi network to endosomes and then lysosomes. Following activation of platelets, T-cells, neutrophils, or endothelium, LAMP1 and LAMP2 are expressed on the cell surface. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and loss of LAMP2 expression is associated with impaired autophagy observed in Danons disease. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunocytochemistry analysis of CHO K1 cells incubated with LAMP1, mAb (Ly1C6) at 1:100 and goat anti-mouse Alexa 488 secondary antibody (Image provided by Dr. Guy Groblewski, University of Wisconsin-Madison).
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Product Literature References
Disruption of Golgi markers by two RILP-directed shRNAs in neurons: a new role for RILP or a neuron-specific off-target phenotype?: C.C. Yap, et al.; bioRxiv (2023),
Abstract;
DEPDC5-dependent mTORC1 signaling mechanisms are critical for the anti-seizure effects of acute fasting: C.J. Yuskaitis, et al.; Cell Rep.
40, 111278 (2022),
Abstract;
Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes: C.C. Yap, et al.; J. Cell Biol.
217, 3141 (2018),
Abstract;
Full Text
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes: A.C. Wolfes, et al.; J. Gen Physiol.
149, 149 (2017),
Abstract;
Full Text
The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes: C.C. Yap, et al.; Sci. Rep.
7, 10481 (2017),
Application(s): IF,
Abstract;
Full Text
Micellar nanocomplexes for biomagnetic delivery of intracellular proteins to dictate axon formation during neuronal development: G. Suarato, et al.; Biomaterials
112, 176 (2016),
Application(s): Examination of NCs localization following uptake,
Abstract;
The novel monoclonal antibody 9F5 reveals expression of a fragment of GPNMB/osteoactivin processed by furin-like protease(s) in a subpopulation of microglia in neonatal rat brain: K. Kawahara, et al.; Glia
64, 1938 (2016),
Application(s): Immunocytochemical Analysis,
Abstract;
Full Text
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion: Y. Hou, et al.; PLoS One
10, e0125596 (2015),
Abstract;
Full Text
Acute mechanisms underlying antibody effects in anti-N-methyl-D-aspartate receptor encephalitis: E.H. Moscato, et al.; Ann. Neurol.
76, 108 (2014),
Abstract;
Full Text
STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy: J.V. Ferreira, et al.; Autophagy
9, 1349 (2013),
Abstract;
Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation: Y. Rong, et al.; Nat. Cell Biol.
14, 924 (2012),
Abstract;
Tumor protein D52 expression and Ca2+-dependent phosphorylation modulates lysosomal membrane protein trafficking to the plasma membrane: D.D. Thomas, et al.; Am. J. Physiol. Cell Physiol.
298, C725 (2010),
Application(s): ICC in CHO-K1 cells,
Abstract;
Full Text
ARAP1 Regulates EGF Receptor Trafficking and Signalling: T. Daniele, et al.; Traffic
9, 2221 (2008),
Application(s): EM using rat cell lysates,
Abstract;
Constitutive, agonist-accelerated, recycling and lysosomal degradation of GABA(B) receptors in cortical neurons: D. Benke, et al. ; Mol. Cell. Neurosci.
39, 628 (2008),
Application(s): IF using rat tissue culture,
Abstract;
The H+-coupled electrogenic lysosomal amino acid transporter LYAAT1 localizes to the axon and plasma membrane of hippocampal neurons: R.H. Edwards, et al. ; J. Neurosci.
23, 1265 (2003),
Application(s): ICC using rat samples,
Abstract;
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) causes Akt phosphorylation and morphological changes in intracellular organellae in cultured rat astrocytes: M. Michikawa, et al. ; J. Neurochem.
77, 274 (2001),
Application(s): ICC using rat samples,
Abstract;
Synapse-associated protein97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway: R.J. Wenthold, et al. ; J. Neurosci.
21, 7506 (2001),
Application(s): ICC using rat samples,
Abstract;
Transport of the lysosomal membrane glycoprotein lgp120 (lgp-A) to lysosomes does not require appearance on the plasma membrane: C. Harter & I. Mellman; J. Cell Biol.
117, 311 (1992),
Application(s): ICC using rat samples,
Abstract;
Glycoproteins of the lysosomal membrane: V. Lewis, et al.; J. Cell Biol.
100, 1839 (1985),
Application(s): ICC, IP using rat samples,
Abstract;
