Product Details
| Alternative Name: | Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine |
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| Clone: | KR-10 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to a portion of bovine KDEL Receptor. |
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| UniProt ID: | P33946 (KDELR1), Q2KJ37 (KDELR2) |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human, Mouse, Rat
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| Applications: | IHC, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:250, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~23kDa by Western blot. |
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| Purity Detail: | Ascites. |
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| Formulation: | Liquid. Mouse ascites fluid containing 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus. |
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| Technical Info/Product Notes: | Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000105438). |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis: Lane 1: MW marker, Lane 2: 20µg of HeLa cell lysate (heat shocked), Lane 3: 20µg of 3T3 cell lysate, Lane 4: 20µg of PC-12 cell lysate, Lane 5: 20µg of CHO-K1 cell lysate, Lane 6: 20µg of 3T3 cell lysate (heat shocked), Lane 7: 20µg of PC-12 cell lysate (heat shocked) probed with KDEL Receptor mAb at 1:250.
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Product Literature References
Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques: A. Pannek, et al.; Mol. Biol. Cell
33, ar6 (2022),
Abstract;
Developmental Regulation and Induction of Cytochrome P450 2W1, an Enzyme Expressed in Colon Tumors: E. Choong, et al.; PLoS One
10, e0122820 (2015),
Application(s): Immunohistochemistry,
Abstract;
Full Text
A novel plasma membrane quinone reductase and NAD(P)H:quinone oxidoreductase 1 are upregulated by serum withdrawal in human promyelocytic HL-60 cells: J.M. Villalba, et al. ; J. Bioenerg. Biomembr.
34, 209 (2002),
Application(s): WB using human samples,
Abstract;
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