Product Details
| Alternative Name: | STIP1, STI1, STUB1, p60, CHIP |
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| Clone: | DS14F5 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Chicken HOP (p60). |
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| UniProt ID: | P31948 |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Mink, Monkey, Porcine, Rabbit, Sheep, Xenopus
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| Applications: | IHC, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~60kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Hsp70-Hsp90 Organizing Protein (HOP, p60) is an ~60kDa protein that is a critical intermediate component for the efficient maturation of steroid receptor complexes, serving to recruit Hsp90 to Hsp70-containing complexes. HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a, and TPR2b. |
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| Technical Info/Product Notes: | Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000168439). |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Phosphorylation of the Hsp90 Co-Chaperone Hop Changes its Conformational Dynamics and Biological Function: M. Castelli, et al.; J. Mol. Biol.
435, 167931 (2023),
Abstract;
Evodiamine inhibits both stem cell and non-stem-cell populations in human cancer cells by targeting heat shock protein 70: S.Y. Hyun, et al.; Theranostics
11, 2932 (2021),
Application(s): Western Blot,
Abstract;
Full Text
The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma: M. Heider, et al.; Mol. Cell
81, 1170 (2021),
Abstract;
Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE: K. Saito, et al.; Eur. J. Immunol.
45, 2028 (2015),
Abstract;
Full Text
Psoriasis-associated variant Act1 D10N with impaired regulation by Hsp90: C. Wang, et al.; Nat. Immunol.
14, 72 (2013),
Application(s): WB using MEF extracts,
Abstract;
Full Text
Hsp90 charged-linker truncation reverses the functional consequences of weakened hydrophobic contacts in the N domain: S. Tsutsumi, et al. ; Nat. Struct. Mol. Biol.
16, 1141 (2009),
Application(s): WB, IP using mouse cell lysates,
Abstract;
Novel oxime derivatives of radicicol induce erythroid differentiation associated with preferential G(1) phase accumulation against chronic myelogenous leukemia cells through destabilization of Bcr-Abl with Hsp90 complex: Y. Shiotsu, et al.; Blood
96, 2284 (2000),
Application(s): WB using human samples,
Abstract;
Quantitative assessment of complex formation of nuclear-receptor accessory proteins: A. Jungbauer, et al. ; Biochem. J.
345, 627 (2000),
Application(s): WB using human samples,
Abstract;
Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly: D.F. Smith, et al. ; Mol. Cell. Biol.
18, 944 (1998),
Application(s): IP using rabbit samples,
Abstract;
The role of DnaJ-like proteins in glucocorticoid receptor.hsp90 heterocomplex assembly by the reconstituted hsp90.p60.hsp70 foldosome complex: W.B. Pratt, et al. ; J. Biol. Chem.
273, 7358 (1998),
Application(s): IP using rabbit samples,
Abstract;
Proteins interacting with the molecular chaperone hsp70/hsc70: physical associations and effects on refolding activity: U. Gehring, et al. ; FEBS Lett.
417, 109 (1997),
Application(s): WB using human samples,
Abstract;
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