Product Details
Alternative Name: | ESR |
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Clone: | C-542 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of human Estrogen Receptor α. |
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UniProt ID: | P03372 |
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GenBank ID: | M12674 |
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Source: | Purified from ascites. |
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Species reactivity: | Human, Mouse, Rat Hamster
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Applications: | ICC, IHC, IP, WB
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Recommended Dilutions/Conditions: | Western Blot (1:500, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of 66.3kDa by Western blot. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues. |
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Regulatory Status: | RUO - Research Use Only |
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Human Estrogen Alpha Receptor domains.
Western blot analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).
Immunohistochemistry analysis of human Breast tumor tissue stained with Estrogen receptor α monoclonal antibody (C-542), (Prod. No. ADI-SRA-1010), at 20µg/ml.
Immunocytochemistry staining of HepG2 cells (Panel A) and MCF-7 (Panel B) with Estrogen receptor α monoclonal antibody (C-542) (Prod. No. ADI-SRA-1010) at 10µg/ml.
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Product Literature References
Chronic exposure to benzyl butyl phthalate (BBP) alters social interaction and fear conditioning in male adult rats: alterations in amygdalar MeCP2, ERK1/2 and ERα: A. Betz, et al.; Neuro. Endocrinol. Lett.
34, 347 (2013),
Abstract;
Reassessment of estrogen receptor expression in human breast cancer cell lines: C.H. Ford, et al.; Anticancer Res.
31, 521 (2011),
Application(s): Flow cytometry and immunofluorescence,
Abstract;
Membrane estrogen receptor-α levels in MCF-7 breast cancer cells predict cAMP and proliferation responses: D. Zivadinovic, et al.; Breast Cancer Res.
7, R101 (2005),
Application(s): Cell immunoseparation,
Abstract;
Regulation of the membrane estrogen receptor-alpha: role of cell density, serum, cell passage number, and estradiol: C.S. Watson, et al. ; FASEB J.
16, 1917 (2002),
Application(s): ICC using rat samples,
Abstract;
The novel isoform of the estrogen receptor-alpha cDNA (ERalpha isoform S cDNA) in the human testis: K. Hoshi, et al. ; J. Steroid Biochem. Mol. Biol.
80, 299 (2002),
Application(s): WB using human samples,
Abstract;
Functional interactions between the estrogen receptor and DRIP205, a subunit of the heteromeric DRIP coactivator complex: L.P. Freedman, et al. ; J. Biol. Chem.
275, 20928 (2000),
Application(s): IP, WB using human samples,
Abstract;
Estrogen receptor-dependent regulation of sensory neuron survival in developing dorsal root ganglion: D. Lindholm, et al. ; PNAS
96, 10905 (1999),
Application(s): ICC using rat samples,
Abstract;
Characterization of Brx, a novel Dbl family member that modulates estrogen receptor action: J. Segars, et al. ; Oncogene
16, 2513 (1998),
Application(s): IP, IHC using human samples,
Abstract;
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