Product Details
Alternative Name: | MtHsp70, Hsp70 (mitochondrial), HspA9 |
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Clone: | 30A5 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Native human Grp75 (Mortalin). |
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UniProt ID: | P38646 |
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GenBank ID: | L15189 |
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Source: | Purified from mouse ascites. |
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Species reactivity: | Human, Mouse, Rat Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
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Applications: | IHC, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (1:50) Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~75kDa by Western blot. |
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Purity Detail: | Ammonium sulfate precipitation purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Grp75 (Glucose regulated 75 kDa protein) is a member of the Hsp70 family. It is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Grp75/Mortalin, mAb (30A5) (Prod. no. ADI-SPS-825): Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: L-929 (heat shocked); Lane 4: CHO -K1 (heat shocked); Lane 5: GPC-16 (heat shocked); Lane 6: PC-12 (heat shocked).
Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).
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Product Literature References
Toxic Memory' via chaperone modification is a potential mechanism for rapid Mallory-Denk body reinduction: M. Omary, et al. ; Hepatology
48, 931 (2008),
Application(s): WB using mouse tissue,
Abstract;
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