Product Details
| Alternative Name: | HSTF1, Heat shock factor protein 1 |
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| Host: | Rabbit |
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| Immunogen: | Recombinant human HSF1. |
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| UniProt ID: | Q00613 |
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| GenBank ID: | M64673 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Gerbil, Monkey, Rabbit
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| Applications: | ELISA, IP, WB
EMSA
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects bands ~80 to ~95kDa (depending on phosphorylation status) by Western blot analysis. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution. |
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| Technical Info/Product Notes: | ADI-SPA-901 is tested against HeLa and HeLa Heat-Shocked lysates. Upon heat shock, HSF1 is hyperphosphorylated. ADI-SPA-901 recognizes both phosphorylated and non-phosporylated HSF1 in Western Blot. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant) (Prod. No. ADI-SPP-900), Lane 3: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100), Lane 4: HeLa Cell Lysate (Heat Shocked) (Prod. No. ADI-LYC-HL101), Lane 5: 3T3 Cell Lysate (Prod. No. ADI-LYC-3T100), Lane 6: PC-12 Cell Lysate (Prod. No. ADI-LYC-PC100), Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate
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Product Literature References
Heat shock factor 1 induces a short burst of transcription of the clock gene Per2 during interbout arousal in mammalian hibernation: N. Takamatsu, et al.; J. Biol. Chem.
299, 104576 (2023),
Abstract;
Accumulation of misfolded SOD1 outlines distinct patterns of motor neuron pathology and death during disease progression in a SOD1G93A mouse model of amyotrophic lateral sclerosis: S. Salvany, et al.; Brain Pathol.
1111, bpa.13078 (2022),
Abstract;
Curcumin increases heat shock protein 70 expression via different signaling pathways in intestinal epithelial cells: G. Mingzu, et al.; Arch. Biochem. Biophys.
707, 108938 (2021),
Abstract;
Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2: L. Pesonen, et al.; Cell Stress Chaperones
26, 819 (2021),
Application(s): Western Blot,
Abstract;
Heat shock factor 1 suppression induces spindle abnormalities and sensitizes cells to antimitotic drugs: H.H. Kuo, et al.; Cell Div.
16, 8 (2021),
Abstract;
The heat shock response, determined by QuantiGene multiplex, is impaired in HD mouse models and not caused by HSF1 reduction: C. Gomez-Paredes, et al.; Sci. Rep.
11, 9117 (2021),
Abstract;
Modulation of Plasma Membrane Composition and Microdomain Organization Impairs Heat Shock Protein Expression in B16-F10 Mouse Melanoma Cells: T. Crul, et al.; Cells
9, 951 (2020),
Abstract;
Full Text
Pro-death signaling of cytoprotective heat shock factor 1: upregulation of NOXA leading to apoptosis in heat-sensitive cells: P. Janus, et al.; Cell Death Differ.
27, 2280 (2020),
Abstract;
Full Text
SRSF3 Is a Critical Requirement for Inclusion of Exon 3 of BIS Pre-mRNA: J.Y. Baek, et al.; Cells
9, 2325 (2020),
Application(s): WB,
Abstract;
Full Text
Histone deacetylase inhibitor SAHA treatment prevents the development of heart failure after myocardial infarction via an induction of heat-shock proteins in rats: S. Nagata, et al.; Biol. Pharm. Bull.
42, 453 (2019),
Abstract;
IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A: T. Ueda, et al.; Cell. Signal.
55, 81 (2019),
Abstract;
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
15, 909 (2018),
Abstract;
Induction of suppressor of cytokine signaling 3 via HSF-1-HSP70-TLR4 axis attenuates neuroinflammation and ameliorates postoperative pain: Y.X. Fan, et al.; Brain Behav. Immun.
68, 111 (2018),
Abstract;
mTORC2/AKT/HSF1/HuR constitute a feed-forward loop regulating Rictor expression and tumor growth in glioblastoma: B. Holmes, et al.; Oncogene
37, 732 (2018),
Abstract;
Full Text
zHSF1 modulates zper2 expression in zebrafish embryos: L. Mennetrier, et al.; Chronobiol. Int.
6, 1 (2018),
Abstract;
Transcriptional regulatory logic of the diurnal cycle in the mouse liver: J.A. Sobel, et al.; PLoS Biol.
15, e2001069 (2017),
Abstract;
Full Text
BIIB021, a synthetic Hsp90 inhibitor, induces mutant ataxin-1 degradation through the activation of heat shock factor 1: Y. Ding, et al.; Neuroscience
327, 20 (2016),
Application(s): Western blot,
Abstract;
ChIP - Does it work correctly? The optimization steps of chromatin immunoprecipitation: M. Kus-Liskiewicz; Acta Biol. Hung.
67, 373 (2016),
Abstract;
Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins: S. Karvinen, et al.; Exp. Gerontol.
79, 46 (2016),
Application(s): Immunoblotting,
Abstract;
The Helicobacter pylori cytotoxin CagA is essential for suppressing host heat shock protein expression: B.J. Lang, et al.; Cell Stress Chaperones
21, 523 (2016),
Abstract;
Full Text
Active heat shock transcription factor 1 supports migration of the melanoma cells via vinculin down-regulation: A. Tomo-Jonik, et al.; Cell. Signal.
27, 394 (2015),
Abstract;
Cross talk between cytokine and hyperthermia-induced pathways: identification of different subsets of NF-κB-dependent genes regulated by TNFα and heat shock: P. Janus, et al.; Mol. Genet. Genomics
290, 1979 (2015),
Abstract;
Full Text
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta
1853, 996 (2015),
Application(s): Western Blotting,
Abstract;
Lysine Deacetylases Regulate the Heat Shock Response Including the Age-Associated Impairment of HSF1: E. Zelin, et al.; J. Mol. Biol.
427, 1644 (2015),
Abstract;
Full Text
NZ28-induced inhibition of HSF1, SP1 and NF-κB triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells: D. Schilling, et al.; Cancer Immunol. Immunother.
64, 599 (2015),
Abstract;
Full Text
Thiopental protects human neuroblastoma cells from apoptotic cell death — Potential role of heat shock protein 70: M. Roesslein, et al.; Life Sci.
139, 40 (2015),
Application(s): Western blot,
Abstract;
Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes: J. Korfanty, et al.; Int. J. Biochem. Cell Biol.
57, 76 (2014),
Abstract;
Differential translocation of heat shock factor-1 after mild and severe stress to human skin fibroblasts undergoing aging in vitro: D. Demirovic, et al.; J. Cell Commun. Signal.
8, 333 (2014),
Abstract;
Full Text
HSF1 deficiency and impaired HSP90-dependent protein folding are hallmarks of aneuploid human cells: N. Donnelly, et al.; EMBO J.
33, 2374 (2014),
Abstract;
Full Text
Novel isoforms of heat shock transcription factor 1, HSF1γα and HSF1γβ, regulate chaperone protein gene transcription: A. Neueder, et al.; J. Biol. Chem.
289, 19894 (2014),
Abstract;
Full Text
Cytotoxicity of withaferin A in glioblastomas involves induction of an oxidative stress-mediated heat shock response while altering Akt/mTOR and MAPK signaling pathways: P.T. Grogan, et al.; Invest. New Drugs
31, 545 (2013),
Abstract;
Full Text
Dynamics of the Full Length and Mutated Heat Shock Factor 1 in Human Cells: G. Herbomel, et al.; PLoS One
8, e67566 (2013),
Abstract;
Full Text
OLA1 protects cells in heat shock by stabilizing HSP70: R.F. Mao, et al.; Cell Death Dis.
14, e491 (2013),
Abstract;
Full Text
Overexpression of heat shock transcription factor 1 enhances the resistance of melanoma cells to doxorubicin and paclitaxel: N. Vydra, et al.; BMC Cancer
13, 504 (2013),
Abstract;
Full Text
The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response: R. Raynes, et al.; PLoS One
8, e54364 (2013),
Abstract;
Full Text
Expression of heat shock transcription factor 1 and its downstream target protein T-cell death associated gene 51 in the spinal cord of a mouse model of amyotrophic lateral sclerosis: T. Mimoto, et al.; Brain Res.
1488, 123 (2012),
Application(s): Immunohistochemistry and western-blotting using mouse neurons,
Abstract;
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell
134, 769 (2008),
Application(s): WB using human cell lysates,
Abstract;
Postinsult treatment with lithium reduces brain damage and facilitates neurological recovery in a rat ischemia/reperfusion model: D.M. Chuang, et al. ; PNAS
100, 6210 (2003),
Application(s): EIA using rat samples,
Abstract;
TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response: G. Steiner, et al. ; Cell Death Differ.
10, 1126 (2003),
Application(s): EMSA using human samples,
Abstract;
17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons: F.R. Sharp, et al. ; J. Cereb. Blood Flow Metab.
22, 183 (2002),
Application(s): IHC using rat & gerbil samples,
Abstract;
Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex: R. Voellmy, et al. ; J. Biol. Chem.
276, 45791 (2001),
Application(s): IP using human samples,
Abstract;
Role of cyclopentenone prostaglandins in rat carrageenin pleurisy: M. Di Rosa, et al. ; FEBS Lett.
508, 61 (2001),
Application(s): EMSA, WB using rat samples,
Abstract;
Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90 beta gene promoters: D.S. Latchman, et al. ; J. Biol. Chem.
274, 1723 (1999),
Application(s): IP using human samples,
Abstract;
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