Product Details
Alternative Name: | Heat shock protein 65 |
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Clone: | 3F7 |
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Host: | Mouse |
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Isotype: | IgM |
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Immunogen: | Recombinant M. bovis HSP65. |
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UniProt ID: | P0A521 |
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Source: | Purified from mouse ascites. |
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Species reactivity: | Human, Mouse, Rat Bovine, Cryptosporidium, Drosophila, E. coli, Guinea pig, Hamster, Mycobacterium bovis, Mycobacterium leprae
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Applications: | WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~65kDa by Western blot. |
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Purity Detail: | Ammonium sulfate precipitation purified. |
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Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | Hsp65 is a member of the Hsp60 family of heat shock proteins isolated from Mycobacterium bovis BCG. Hsp65 from M. bovis is identical to that of M. tuberculosis, and similar to that of M. leprae, two highly pathogenic strains of mycobacterium. Hsp65 is the immunodominant antigen during mycobacterial infection and vaccination, and has been linked to the development of autoimmune adjuvant arthritis in rats. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E. coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant Mycobacterium bovis Protein, Lane 5: HeLa (HS) Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: 3T3 Cell Lysate.
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Product Literature References
gamma delta+ T cells and 65-kDa heat shock protein expression following Cryptosporidium parvum challenge in athymic C57BL/6J nude mice: F.J. Enriquez, et al. ; Immunol. Lett.
72, 35 (2000),
Application(s): WB using cryptosporidium samples,
Abstract;
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