Product Details
| Alternative Name: | HspA5, HSP70-5, Glucose-regulated protein 78, Heat shock 70kDa protein 5 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of rat Grp78 (BiP). The sequence is completely conserved in mouse and hamster. |
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| UniProt ID: | P06761 |
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| GenBank ID: | M14050 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Mouse, Rat
Bovine, Fungus, Hamster, Monkey, Rabbit, Xenopus
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~78kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Grp78 (Glucose Regulated 78kDa Protein), is also known as BiP or Immunoglobulin heavy chain binding protein. It is a stressresponse protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding, and assembly of many membrane bound and secreted proteins as well as for maintenance of cell homeostasis and the prevention of apoptosis. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis: Lane 1: MW marker, Lane 2: 150 ng of Grp78 (BiP) recombinant protein (Prod. No. ADI-SPP-765), Lane 3: 20µg of CHO-K1 cell lysate, Lane 4: 20µg of mouse liver microsome tissue extract (Prod. No. ADI-LYT-MM100).
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Product Literature References
CHIP Haploinsufficiency Exacerbates Hepatic Steatosis via Enhanced TXNIP Expression and Endoplasmic Reticulum Stress Responses: J.H. Han, et al.; Antioxidants
12, 458 (2023),
Abstract;
Improved skeletal muscle fatigue resistance in experimental autoimmune myositis mice following high-intensity interval training: T. Yamdaa, et al.; Arthritis Res. Ther.
24, 156 (2022),
Abstract;
Vaspin attenuates steatosis-induced fibrosis via GRP78 receptor by targeting AMPK signaling pathway: A. Abdolahi, et al.; J. Physiol. Biochem.
78, 185 (2022),
Abstract;
Ketamine and Xylazine Effects in Murine Model of Acute Pancreatitis: M. Wang & F.S. Gorelick; Am. J. Physiol. Gastrointest. Liver Physiol.
1152, 23 (2021),
Abstract;
Glucose-Regulated Protein 78 in the Aqueous Humor of Patients with Diabetic Macular Edema: M.Y. Choi, et al.; J. Diabetes Res.
2020, 1640162 (2020),
Abstract;
Full Text
Targeting N-myristoylation for therapy of B-cell lymphomas: E. Beauchamp, et al.; Nat. Commun.
11, 5348 (2020),
Abstract;
Full Text
Metabolic Changes in Spontaneously Hypertensive Rat Hearts Precede Cardiac Dysfunction and Left Ventricular Hypertrophy: J. Li, et al.; J. Am. Heart Assoc.
8, e010926 (2019),
Abstract;
Full Text
Mutant huntingtin inhibits the mitochondrial unfolded protein response by impairing ABCB10 mRNA stability: Z. Fu, et al.; Biochim. Biophys. Acta Mol. Basis Dis.
1865, 1428 (2019),
Abstract;
High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect: D. Medina-Cano, et al.; Elife
7, e38309 (2018),
Abstract;
Full Text
Inhibiting the integrated stress response pathway prevents aberrant chondrocyte differentiation thereby alleviating chondrodysplasia: C. Wang, et al.; Elife
7, e37673 (2018),
Abstract;
Full Text
Interactome Screening Identifies the ER Luminal Chaperone Hsp47 as a Regulator of the Unfolded Protein Response Transducer IRE1α: D. Sepulveda, et al.; Mol. Cell
69, 238 (2018),
Abstract;
Involvement of Up-Regulation of DR5 Expression and Down-Regulation of c-FLIP in Niclosamide-Mediated TRAIL Sensitization in Human Renal Carcinoma Caki Cells: J.M. Yun, et al.; Molecules
23, E2264 (2018),
Abstract;
Full Text
Protective effect of 4-Phenylbutyrate against proteolipid protein mutation-induced endoplasmicreticulum stress and oligodendroglial cell death: A.S. Wilding, et al.; Neurochem. Int.
118, 185 (2018),
Abstract;
SIRT1 activation with neuroheal is neuroprotective but SIRT2 inhibition with AK7 is detrimental for disconnected motoneurons: D. Romeo-Guitartet, al.; Cell Death Dis.
9, 531 (2018),
Abstract;
Full Text
HSPB1 facilitates ERK-mediated phosphorylation and degradation of BIM to attenuate endoplasmic reticulum stress-induced apoptosis: D. Kennedy, et al.; Cell Death Dis.
8, e3026 (2017),
Abstract;
Full Text
α7β1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise: Z.S. Mahmassani, et al.; Am. J. Physiol. Cell Physiol.
312, C638 (2017),
Abstract;
Full Text
Activation of the unfolded protein response promotes axonal regeneration after peripheral nerve injury: M. Onate, et al.; Sci. Rep.
6, 21709 (2016),
Application(s): Western blot,
Abstract;
Full Text
Apratoxin A Shows Novel Pancreas-Targeting Activity through the Binding of Sec 61: K.C. Huang, et al.; Mol. Cancer Ther.
15, 1208 (2016),
Abstract;
The HSP90 inhibitor, 17AAG, protects the intestinal stem cell niche and inhibits graft versus host disease development: A.L. Joly, et al.; Oncogene
35, 2842 (2016),
Application(s): Western Blot,
Abstract;
A Versatile Cell Death Screening Assay Using Dye-Stained Cells and Multivariate Image Analysis: T.J. Collins, et al. ; Assay Drug Dev. Technol.
13, 547 (2015),
Application(s): Western Blot,
Abstract;
Full Text
MiR-184 regulates pancreatic β-cell function according to glucose metabolism: S.G. Tattikota, et al.; J. Biol. Chem.
290, 20284 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Aging and sleep deprivation induce the unfolded protein response in the pancreas: implications for metabolism: N. Naidoo, et al.; Aging Cell
13, 131 (2014),
Abstract;
Full Text
Impact of aging on heat shock protein expression in the substantia nigra and striatum of the female rat: A.M. Gleixner, et al.; Cell Tissue Res.
357, 43 (2014),
Abstract;
PiZ Mouse Liver Accumulates Polyubiquitin Conjugates That Associate with Catalytically Active 26S Proteasomes: C.J. Haddock, et al.; PLoS One
9, e106371 (2014),
Abstract;
Full Text
Inositol-1,4,5-trisphosphate receptor regulates hepatic gluconeogenesis in fasting and diabetes: Y. Wang, et al.; Nature
485, 128 (2012),
Abstract;
Full Text
ERp29, an Endoplasmic Reticulum Secretion Factor Is Involved in the Growth of Breast Tumor Xenografts: S. Mkrtchian, et al. ; Mol. Carcinog.
47, 886 (2008),
Application(s): WB using human tissue culture,
Abstract;
Y Nerve growth factor blocks thapsigargin-induced apoptosis at the level of the mitochondrion via regulation of Bim: A. Samali, et al. ; J. Cell. Mol. Med.
12, 2482 (2008),
Application(s): WB using rat cell lysates,
Abstract;
Small heat-shock proteins select DeltaF508-CFTR for endoplasmic reticulum-associated degradation: J. Brodsky, et al. ; Mol. Biol. Cell
18, 806 (2007),
Application(s): WB using human cell lysates,
Abstract;
Bax plays a pivotal role in thapsigargin-induced apoptosis of human colon cancer HCT116 cells by controlling Smac/Diablo and Omi/HtrA2 release from mitochondria: H.G. Wang, et al. ; Cancer Res.
63, 1483 (2003),
Application(s): WB using human samples,
Abstract;
gamma-Secretase activity requires the presenilin-dependent trafficking of nicastrin through the Golgi apparatus but not its complex glycosylation: B. De Strooper, et al. ; J. Cell. Sci.
116, 1127 (2003),
Application(s): ICC using mouse samples,
Abstract;
Mutant SOD1 linked to familial amyotrophic lateral sclerosis, but not wild-type SOD1, induces ER stress in COS7 cells and transgenic mice: T. Kato, et al. ; Biochem. Biophys. Res. Commun.
303, 496 (2003),
Application(s): ICC, WB, IHC using monkey samples,
Abstract;
Differential interaction of molecular chaperones with procollagen I and type IV collagen in corneal endothelial cells: E.P. Kay, et al. ; Mol. Vis.
8, 9 (2002),
Application(s): ICC using rabbit samples,
Abstract;
Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP: M. Mori, et al. ; J. Biol. Chem.
277, 12343 (2002),
Application(s): WB using mouse samples,
Abstract;
The protein kinase/endoribonuclease IRE1 alpha that signals the unfolded protein response has a luminal N-terminal ligand-independent dimerization domain: R.J. Kaufman, et al. ; J. Biol. Chem.
277, 18346 (2002),
Application(s): IP using human & monkey samples,
Abstract;
Truncation releases olfactory receptors from the endoplasmic reticulum of heterologous cells: T.S. McClintock, et al. ; J. Neurochem.
72, 2301 (1999),
Application(s): ICC using human & xenopus samples,
Abstract;
General Literature References
The critical roles of endoplasmic reticulum chaperones and unfolded protein response in tumorigenesis and anticancer therapies: B. Luo & A.S. Lee; Oncogene
32, 805 (2013), (Review),
Abstract;
Full Text
The critical role of GRP78 in physiologic and pathologic stress: K.T. Pfaffenbach & A.S. Lee; Curr. Opin. Cell Biol.
23, 150 (2011), (Review),
Abstract;
Full Text
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