Product Details
| Alternative Name: | Heat shock protein 70, Hsc70, Hsp73 |
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| Clone: | 5B7 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant spinach Hsc70 (Hsp73). |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Plant
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| Applications: | IP, WB
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| Application Notes: | Detects a band of ~70kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of HSC70: Lane 1: MW marker, Lane 2: DnaK Recombinant E. coli Protein, Lane 3: Banana Lysate, Lane 4: Orange Lysate, Lane 5: Carrot Lysate, Lane 6: Cytosolic wheat HSC70.
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Product Literature References
A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling: K.L. Gonzalez, et al.; PNAS
115, E3163 (2018),
Abstract;
Full Text
Pexophagy and peroxisomal protein turnover in plants: P.G. Young & B. Bartel; Biochim. Biophys. Acta
1863, 999 (2016),
Application(s): Western Blot,
Abstract;
Structural organization of the spinach endoplasmic reticulum-luminal 70-kilodalton heat-shock cognate gene and expression of 70-kilodalton heat-shock genes during cold acclimation: C.L. Guy, et al. ; Plant Physiol.
104, 1359 (1994),
Application(s): IP, WB using plant (spinach) samples,
Abstract;
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