ELISA, Flow Cytometry, ICC, IF, IHC, IP, WB Electron microscopy
Recommended Dilutions/Conditions:
Immunohistochemistry (1:50)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~70kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Immunohistochemistry analysis of human colon cancer tissue using HSP70 mAb (C92F3A-5) at a dilution of 1:50.
Substrate binding domain of HSP70 in complex with a substrate peptide10.
Western blot analysis of HSP70/HSP72, mAb (C92F3A-5) (Prod. No. ADI-SPA-810): Lane 1: MW marker, Lane 2: HSP70/HSP72 (human), (recombinant) (Prod. No. ADI-NSP-555), Lane 3: HSC70/HSP73 (bovine), (recombinant) (Prod. No. ADI-SPP-751) (Negative Control), Lane 4: PC-12 (heat shocked) (Prod. No. ADI-ADI-LYC-PC101), Lane 5: HeLa (heat shocked) (Prod. No. ADI-LYC-HL101), Lane 6: 3T3 (heat shocked) (Prod. No. ADI-LYC-3T101), Lane 7: CHO-K1 (heat shocked).
Please mouse over
Product Literature References
Azaphilones produced by Penicillium maximae with their cell death-inducing activity on Adriamycin-treated cancer cell: T. Matsumoto, et al.; Genes Environ. 45, 5 (2023), Abstract;
Calmodulin as a Key Regulator of Exosomal Signal Peptides: K. Ono, et al.; Cells 12, 158 (2023), Abstract;
Cytosolic stress granules relieve the ubiquitin-proteasome system in the nuclear compartment: S. Xu, et al.; EMBO J. 42, e111802 (2023), Abstract;
Efficient cell death mediated by bioengineered killer extracellular vesicles: J. Dancourt, et al.; Sci. Rep. 13, 1086 (2023), Abstract;
HSP70 mediates a crosstalk between the estrogen and the heat shock response pathways: M.A.D. Silveira, et al.; J. Biol. Chem. 299, 102872 (2023), Abstract;
Methylmercury-induced reactive oxygen species-dependent and independent dysregulation of MAP kinase-related signaling pathway in cultured normal rat cerebellar astrocytes: S. Sasaki, et al.; Toxicology 487, 153463 (2023), Abstract;
N-butyrate increases heat shock protein 70 through heat shock factor 1 and AMP-activated protein kinase pathways in human intestinal Caco-2 cells: P.A. Adesina, et al.; Arch. Biochem. Biophys. 736, 109525 (2023), Abstract;
Virus-Free Method to Control and Enhance Extracellular Vesicle Cargo Loading and Delivery: S. Bui, et al.; ACS Appl. Bio. Mater. 6, 1081 (2023), Abstract;
A novel C-terminal heat shock protein 90 inhibitor that overcomes STAT3-Wnt-β-catenin signaling-mediated drug resistance and adverse effects: H.J. Lee, et al.; Theranostics 12, 105 (2022), Abstract;
Alterations in Proteostasis System Components in Peripheral Blood Mononuclear Cells in Parkinson Disease: Focusing on the HSP70 and p62 Levels: J.D. Vavilova, et al.; Biomolecules 12, 493 (2022), Abstract;
Distinct Cellular Tools of Mild Hyperthermia-Induced Acquired Stress Tolerance in Chinese Hamster Ovary Cells: Á. Tiszlavicz, et al.; Biomedicines 10, 1172 (2022), Abstract;
Factors Associated with Development of Acute Kidney Injury After Liver Transplantation: R. Catalan, et al.; Rev. Invest. Clin. 74, 090 (2022), Abstract;
Hepatocyte growth factor reverses cholemic nephropathy associated with α-naphthylisothiocyanate-induced cholestasis in mice: S. Salas-Silva, et al.; Life Sci. 295, 120423 (2022), Abstract;
Hepatocyte-specific deletion of Bis causes senescence in the liver without deteriorating hepatic function: S.Y. Jung, et al.; Biochem. Biophys. Res. Commun. 619, 42 (2022), Abstract;
Hsp72-Based Effect and Mechanism of Microwave Radiation-Induced Cardiac Injury in Rats: D. Li, et al.; Oxid. Med. Cell. Longev. 18, 7145415 (2022), Abstract;
Impaired Heat Shock Protein Expression in Activated T Cells in B-Cell Lymphoma: Z. Albakova, et al.; Biomedicines 10, 2747 (2022), Abstract;
Inhaled nitric oxide improves post-cardiac arrest outcomes via guanylate cyclase-1 in bone marrow-derived cells: Y. Miyazaki, et al.; Nitric Oxide 125, 47 (2022), Abstract;
MicroRNA-124-3p-enriched small extracellular vesicles as a therapeutic approach for Parkinson’s disease: M. Esteves, et al.; Mol. Ther. 30, 3176 (2022), Abstract;
Oviductal epithelial cells transcriptome and extracellular vesicles characterization during thermoneutral and heat stress conditions in dairy cows: K. Stamperna, et al. ; Theriogenology 187, 152 (2022), Abstract;
Structure of Hsp90-Hsp70-Hop-GR reveals the Hsp90 client-loading mechanism: R.Y. Wang, et al.; Nature 601, 460 (2022), Abstract;
The Change in Circadian Rhythms in P301S Transgenic Mice is Linked to Variability in Hsp70-related Tau Disaggregation: S.M. Han, et al.; Exp. Neurobiol. 31, 196 (2022), Abstract;
The Therapeutic Roles of Recombinant Hsp90α on Cornea Epithelial Injury: M. Wang, et al.; Invest. Ophthalmol. Vis. Sci. 63, 30 (2022), Abstract;
17β-Estradiol Increases APE1/Ref-1 Secretion in Vascular Endothelial Cells and Ovariectomized Mice: Involvement of Calcium-Dependent Exosome Pathway: Y.R. Lee, et al.; Biomedicines 9, 1040 (2021), Abstract;
Chemical structures and induction of cell death via heat shock protein inhibition of the prenylated phloroglucinol derivatives isolated from Hypericum erectum: T. Μatsumoto, et al.; Fitoterapia 156, 105097 (2021), Abstract;
Development of accurate temperature regulation culture system with metallic culture vessel demonstrates different thermal cytotoxicity in cancer and normal cells: C. Imashiro, et al.; Sci. Rep. 11, 21466 (2021), Abstract;
FOXR1 regulates stress response pathways and is necessary for proper brain development: A. Mota, et al.; PLoS Genet. 17, e1009854 (2021), Abstract;
Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2: L. Pesonen, et al.; Cell Stress Chaperones 26, 819 (2021), Application(s): Western Blot, Abstract;
High-frequency blood flow restricted resistance exercise results in acute and prolonged cellular stress more pronounced in type I than in type II fibers: T. Bjornsen, et al.; J. Appl. Physiol. 131, 643 (2021), Abstract;
HSP70iQ435A to subdue autoimmunity and support anti-tumor responses: D. Jaishankar, et al.; Cell Stress Chaperones 26, 845 (2021), Abstract;
Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice: A.R. Caldwell, et al.; J. Appl. Physiol. 10, 1152 (2021), Abstract;
Novologue Therapy Requires Heat Shock Protein 70 and Thioredoxin-Interacting Protein to Improve Mitochondrial Bioenergetics and Decrease Mitophagy in Diabetic Sensory Neurons: Y.A. Rodriguez, et al.; ACS Chem. Neurosci. 12, 3049 (2021), Abstract;
Polymer-ritonavir derivate nanomedicine with pH-sensitive activation possesses potent anti-tumor activity in vivo via inhibition of proteasome and STAT3 signaling: L. Sivák, et al.; J. Control Release 332, 563 (2021), Application(s): Flow Cytometry on CT26 cells, Abstract;
Regulation of Hippo pathway by Hsp70-Bag3 complex: Bag3 modulates YAP phosphorylation and its nuclear translocation: S. Baldan, et al.; J. Cell Sci. 134, jcs.259107 (2021), Abstract;
Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy: M. Schuldt, et al.; Front. Cardiovasc. Med. 8, 612215 (2021), Abstract;
Urocortin 3 overexpression reduces ER stress and heat shock response in 3T3-L1 adipocytes: S. Kavalakatt, et al.; Sci. Rep. 11, 15666 (2021), Abstract;
Chronic heat stress regulates the relation between heat shock protein and immunity in broiler small intestine: G. Han, et al.; Sci. Rep. 10, 18872 (2020), Abstract; Full Text
Modulation of Plasma Membrane Composition and Microdomain Organization Impairs Heat Shock Protein Expression in B16-F10 Mouse Melanoma Cells: T. Crul, et al.; Cells 9, 951 (2020), Abstract;
Monitoring HSP70 exosomes in cancer patients’ follow up: a clinical prospective pilot study: G. Chanteloup, et al.; J. Extracell. Vesicles 9, 1766192 (2020), Abstract; Full Text
Neuronal specific and non-specific responses to cadmium possibly involved in neurodegeneration: A toxicogenomics study in a human neuronal cell model: M. Forcella, et al.; Neurotoxicology 76, 162 (2020), Abstract;
Nuclear import of IER5 is mediated by a classical bipartite nuclear localization signal and is required for HSF1 full activation: S. Yamano, et al.; Exp. Cell Res. 386, 111686 (2020), Abstract;
Placental Mitochondria Therapy for Cerebral Ischemia-Reperfusion Injury in Mice: Y. Nakamura, et al.; Stroke 51, 3142 (2020), Application(s): WB; mouse placenta, Abstract;
Pro-death signaling of cytoprotective heat shock factor 1: upregulation of NOXA leading to apoptosis in heat-sensitive cells: P. Janus, et al.; Cell Death Differ. 27, 2280 (2020), Abstract; Full Text
αB-crystallin response to a pro-oxidant non-cytotoxic environment in murine cardiac cells: An "in vitro" and "in vivo" study: A. Antonioni, et al.; Free Radic. Biol. Med. 152, 301 (2020), Abstract;
Aggregation of the nucleic acid-binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation: Y. Chen & T.J. Cohen; J. Biol. Chem. 294, 3696 (2019), Abstract;
Celastrol-type HSP90 modulators allow for potent cardioprotective effects: H. Aceros, et al.; Life Sci. 227, 8 (2019), Abstract;
Characterization of heat shock protein 27 in extracellular vesicles: a potential anti-inflammatory therapy: C. Shi, et al.; FASEB J. 33, 1617 (2019), Abstract;
Epileptogenesis-Associated Alterations of Heat Shock Protein 70 in a Rat Post-Status Epilepticus Model: F. Gualtieri, et al.; Neuroscience 415, 44 (2019), Application(s): IHC and IF on free-floating cryosections of rat brain tissue, Abstract;
Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis: J.V. Ferreira, et al.; PLoS One 15, e0223790 (2019), Abstract; Full Text
Hsp70 and Hsp40 inhibit an inter-domain interaction necessary for transcriptional activity in the androgen receptor: B. Eftekharzadeh, et al.; Nat. Commun. 10, 3562 (2019), Abstract; Full Text
Interval running training improves age-related skeletal muscle wasting and bone loss: Experiments with ovariectomized rats: J.S. Kim, et al.; Exp. Physiol. 104, 691 (2019), Abstract;
Modeling of LMNA-Related Dilated Cardiomyopathy Using Human Induced Pluripotent Stem Cells: D. Shah, et al.; Cells 8, 594 (2019), Abstract; Full Text
Novel cell line models to study mechanisms and overcoming strategies of proteasome inhibitor resistance in multiple myeloma: D. Brunnert, et al.; Biochim. Biophys. Acta Mol. Basis Dis. 1865, 1666 (2019), Abstract;
Pharmacological induction of Heat Shock Protein 70 by celastrol protects motoneurons from excitotoxicity in rat spinal cord in vitro: A. Petrovic, et al.; Eur. J. Neurosci. 49, 215 (2019), Abstract;
Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock: M. Azkanaz, et al.; Elife 8, e45205 (2019), Abstract; Full Text
Reduced endothelial nitric oxide synthase activation contributes to cardiovascular injury during chronic kidney disease progression: I. Amador-Martínez, et al.; Am. J. Physiol. Renal Physiol. 317, F275 (2019), Application(s): Western Blot, Abstract;
The Step of Incorporation of Bacillus coagulans GBI-30 6086 Into “requeijão cremoso” Processed Cheese Does Not Affect Metabolic Homeostasis of Rats: M.B. Soares, et al.; Front. Microbiol. 10, 2332 (2019), Abstract; Full Text
2′-hydroxycinnamaldehyde inhibits cancer cell proliferation and tumor growth by targeting the pyruvate kinase M2: Y.J. Yoon, et al.; Cancer Lett. 434, 42 (2018), Abstract;
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Alcohol exposure promotes DNA methyltransferase DNMT3A upregulation through reactive oxygen species-dependent mechanisms: F. Miozzo, et al.; Cell Stress Chaperones 23, 115 (2018), Abstract; Full Text
Co-enzyme Q10 upregulates Hsp70 and protects chicken primary myocardial cells under in vitro heat stress via PKC/MAPK: J. Xu, et al.; Mol. Cell. Biochem. 449, 195 (2018), Abstract;
Combined treatment of human multiple myeloma cells with bortezomib and doxorubicin alters the interactome of 20S proteasomes: A.G. Mittenberg, et al.; Cell Cycle 17, 1745 (2018), Abstract;
Differential expression of heat shock proteins and activation of mitogen-activated protein kinases in A549 alveolar epithelial cells exposed to cigarette smoke extract: A. Somborac-Bacura, et al.; Exp. Physiol. 103, 1666 (2018), Abstract;
Dysregulated autophagy in restrictive cardiomyopathy due to Pro209Leu mutation in BAG3: A. Schanzer, et al.; Mol. Genet. Metab. 123, 388 (2018), Abstract;
Effect of BIS depletion on HSF1-dependent transcriptional activation in A549 non-small cell lung cancer cells: H.H. Yun. et al.; Korean J. Physiol. Pharmacol. 22, 457 (2018), Abstract; Full Text
Effect of Tim23 knockdown in vivo on mitochondrial protein import and retrograde signaling to the UPRmt in muscle: A.N. Oliveira & D.A. Hood; Am. J. Physiol. Cell Physiol. 315, C516 (2018), Abstract;
Genomic analysis of DNA repair genes and androgen signaling in prostate cancer: S. Mukai, et al.; PLoS One 13, e0203742 (2018), Abstract; Full Text
HSC70 is a chaperone for wild-type and mutant cardiac myosin binding protein C: A.A. Glazier, et al.; JCI Insight 3, 99319 (2018), Abstract; Full Text
HSF1 Is Essential for Myeloma Cell Survival and A Promising Therapeutic Target: J.H.L. Fok, et al.; Clin. Cancer Res. 24, 2395 (2018), Abstract;
Impact of Trans-Fats on Heat-Shock Protein Expression and the Gut Microbiota Profile of Mice: G.C.B.C. Carvalho, et al.; J. Food Sci. 83, 489 (2018), Abstract;
Impaired Proteostasis in Senescent Vascular Endothelial Cells: A Perspective on Estrogen and Oxidative Stress in the Aging Vasculature: H.V. Hwang, et al.; Am. J. Physiol. Heart Circ. Physiol. 316, H421 (2018), Abstract;
Medium-chain TAG improve intestinal integrity by suppressing toll-like receptor 4, nucleotide-binding oligomerisation domain proteins and necroptosis signalling in weanling piglets challenged with lipopolysaccharide: X. Xu, et al.; Br. J. Nutr. 6, 1 (2018), Abstract;
Novel role for the testis-enriched HSPA2 protein in regulating epidermal keratinocyte differentiation: A. Gogler-Piglowska, et al.; J. Cell. Physiol. 233, 2629 (2018), Abstract;
Preconditioning contractions prevent the delayed onset of myofibrillar dysfunction after damaging eccentric contractions: R. Yamada, et al.; J. Physiol. 596, 4427 (2018), Abstract; Full Text
Skeletal Muscle-Specific Overexpression of Heat Shock Protein 72 Improves Skeletal Muscle Insulin-Stimulated Glucose Uptake But Does Not Alter Whole Body Metabolism: J. Marshall, et al.; Diabetes Obes. Metab. 20, 1928 (2018), Abstract; Full Text
The role of autophagy in age-related macular degeneration: N. Kivinen, et al.; Acta Opthalmol. 96 Suppl A110, 1 (2018), Abstract; Full Text
Trimethylamine-N-oxide, as a risk factor for atherosclerosis, induces stress in J774A.1 murine macrophages: A. Mohammadi, et al.; Adv. Med. Sci. 63, 57 (2018), Application(s): Western Blot, Abstract;
1,4-Naphthoquinone activates the HSP90/HSF1 pathway through the S-arylation of HSP90 in A431 cells: Negative regulation of the redox signal transduction pathway by persulfides/polysulfides: Y. Abiko, et al.; Free Radic. Biol. Med. 104, 118 (2017), Abstract;
A Topical Zinc Ionophore Blocks Tumorigenic Progression in UV-exposed SKH-1 High-risk Mouse Skin: R. Justiniano, et al.; Photochem. Photobiol. 93, 1472 (2017), Abstract; Full Text
Acute exercise boosts cell proliferation and the heat shock response in lymphocytes: correlation with cytokine production and extracellular-to-intracellular HSP70 ratio: T.G. Heck, et al.; Cell Stress Chaperones 22, 271 (2017), Abstract; Full Text
An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function: D. Mateju, et al.; EMBO J. 36, 1669 (2017), Abstract; Full Text
Cadmium-Mediated Activation of the HSP90/HSF1 Pathway Regulated by Reactive Persulfides/Polysulfides: Y. Shinkai, et al.; Toxicol. Sci. 156, 412 (2017), Abstract; Full Text
Co-enzyme Q10 and acetyl salicylic acid enhance Hsp70 expression in primary chicken myocardial cells to protect the cells during heat stress: J. Xu, et al.; Mol. Cell. Biochem. 435, 73 (2017), Abstract;
Evaluation of geranylgeranylacetone against cisplatin-induced ototoxicity by auditory brainstem response, heat shock proteins and oxidative levels in guinea pigs: W.C. Lo, et al.; Neurotoxicol Teratol. 61, 29 (2017), Abstract;
Hsp70 expression induced by Co-Enzyme Q10 protected chicken myocardial cells from damage and apoptosis under in vitro heat stress: J. Xu, et al.; Poult. Sci. 96, 1426 (2017), Abstract;
Immunoproteomic identification and characterization of Ni2+-regulated proteins implicates Ni2+ in the induction of monocyte cell death: A. Jakob, et al.; Cell Death Dis. 8, e2684 (2017), Abstract; Full Text
Mass spectrometry analyses of normal and polyglutamine expanded ataxin-3 reveal novel interaction partners involved in mitochondrial function: L.V. Kristensen, et al.; Neurochem. Int. 112, 5 (2017), Abstract;
Modulatory effects of arginine, glutamine and branched-chain amino acids on heat shock proteins, immunity and antioxidant response in exercised rats: C.S. Moura, et al.; Food Funct. 8, 3228 (2017), Abstract;
Secretion of cytokines and heat shock protein (HspA1A) by ovarian cancer cells depending on the tumor type and stage of disease: M. Nowak, et al.; Cytokine 89, 136 (2017), Application(s): Western blot, Abstract;
The effects of heat stress on morphological properties and intracellular signaling of denervated and intact soleus muscles in rats: T. Ohira, et al.; Physiol. Rep. 5, e13350 (2017), Abstract; Full Text
The Hsp70 inhibiting peptide aptamer A17 potentiates radiosensitization of tumor cells by Hsp90 inhibition: D. Schilling, et al.; Cancer Lett. 390, 146 (2017), Abstract;
The ubiquitin conjugating enzyme Ube2W regulates solubility of the Huntington's disease protein, huntingtin: B. Wang, et al.; Neurobiol. Dis. 109, 127 (2017), Application(s): Western Blot, Abstract;
Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90: G. Zhang, et al.; Nat. Commun. 8, 589 (2017), Abstract; Full Text
Addition of Alanyl-Glutamine to Dialysis Fluid Restores Peritoneal Cellular Stress Responses - A First-In-Man Trial: K. Kratochwill, et al.; PLoS One 11, e0165045 (2016), Application(s): Western blot, peritoneal cells, Abstract; Full Text
ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation: J.H. Seo, et al.; Nat. Commun. 7, 12882 (2016), Application(s): In vitro acetylation assay, deacetylation assay, binding assay, and ATPase activity assay, Abstract; Full Text
Compression loading-induced stress responses in intervertebral disc cells encapsulated in 3D collagen constructs: W.H. Chooi, et al.; Sci. Rep. 6, 26449 (2016), Application(s): Immunofluorescence staining, Abstract; Full Text
DHA-induced stress response in human colon cancer cells-focus on oxidative stress and autophagy: K. Pettersen, et al.; Free Radic. Biol. Med. 90, 158 (2016), Application(s): Cell culture, Abstract;
Diet-induced elevation of circulating HSP70 may trigger cell adhesion and promote the development of atherosclerosis in rats: F. Xie, et al.; Cell Stress Chaperones 21, 907 (2016), Abstract; Full Text
Different growth and metastatic phenotypes associated with a cell-intrinsic change of Met in metastatic melanoma: E. Adachi, et al.; Oncotarget 7, 70779 (2016), Abstract; Full Text
Dihydropyridine Derivatives Modulate Heat Shock Responses and have a Neuroprotective Effect in a Transgenic Mouse Model of Alzheimer's Disease: A. Kasza, et al.; J. Alzheimers Dis. 53, 557 (2016), Abstract; Full Text
Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins: S. Karvinen, et al.; Exp. Gerontol. 79, 46 (2016), Application(s): Immunoblotting, Abstract;
Functional effects of milk bioactive peptides on skeletal muscle of rats: C.S. Moura, at al.; Food Res. Int. 84, 18 (2016), Application(s): Western blot,
Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping: N. Frara, et al.; BMC Musculoskelet. Dis. 17, 34 (2016), Application(s): Western blot, Abstract; Full Text
HSP90 inhibitors in the context of heat shock and the unfolded protein response: effects on a primary canine pulmonary adenocarcinoma cell line: A.N. Graner, et al.; Int. J. Hyperthermia 20, 1 (2016), Abstract; Full Text
Human CD1c(+) DCs are critical cellular mediators of immune responses induced by immunogenic cell death: S. Di Blasio, et al.; Oncoimmunology 5, e1192739 (2016), Abstract; Full Text
IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis: Y. Asano, et al.; Sci. Rep. 12, 19174 (2016), Application(s): Western blot, Abstract; Full Text
Lysosomal membrane stability plays a major role in the cytotoxic activity of the anti-proliferative agent, di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT): E.M. Gutierrez, et al.; Biochim. Biophys. Acta 1863, 1665 (2016), Application(s): Western blotting and immunoprecipitation, Abstract;
Nondamaging Retinal Laser Therapy: Rationale and Applications to the Macula: D. Lavinsky, et al.; Invest. Ophthalmol. Vis. Sci. 57, 2488 (2016), Application(s): Immunohistochemistry, Abstract; Full Text
Overexpression of heat shock factor 1 maintains TAR DNA binding protein 43 solubility via induction of inducible heat shock protein 70 in cultured cells: P.Y. Lin, et al.; J. Neurosci. Res. 94, 671 (2016), Abstract;
Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells: N.V. Andreeva, et al.; Cell Stress Chaperones 21, 727 (2016), Abstract; Full Text
Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors: A.M. Jaeger, et al.; Nat. Struct. Mol. Biol. 23, 147 (2016), Application(s): Immunoblot analysis, Abstract;
Systematic Proteomic Identification of the Heat Shock Proteins (Hsp) that Interact with Estrogen Receptor Alpha (ERα) and Biochemical Characterization of the ERα-Hsp70 Interaction: A.E. Dhamad, et al.; PLoS One 11, e0160312 (2016), Abstract; Full Text
The Molecular Chaperone Hsp70 Promotes the Proteolytic Removal of Oxidatively Damaged Proteins by the Proteasome: S. Reeg, et al.; Free Radic. Biol. Med. 99, 153 (2016), Application(s): Immunoblot Analysis; Immunoprecipitation of Hsp70 from HT22 cell lysates and murine brain lysates, Abstract;
Tissue oxidative stress induced by Patulin and protective effect of Crocin: M. Boussabbeh, et al.; Neurotoxicology 53, 343 (2016), Abstract;
Acute isoproterenol induces anxiety-like behavior in rats and increases plasma content of extracellular vesicles: G. Leo, et al.; Physiol. Behav. 142C, 79 (2015), Application(s): Western Blot, Abstract;
Androgen Deprivation and Cytoprotective Parameters in Rat Lung: S.M. Álvarez, et al.; IJRSB 3, 150 (2015), Application(s): Immunohistochemistry, Full Text
Autophagic degradation of the androgen receptor mediated by increased phosphorylation of p62 suppresses apoptosis in hypoxia: T. Mitani, et al.; Cell. Signal. 27, 1994 (2015), Application(s): Western Blot, Abstract;
Basal and stress-inducible expression of HSPA6 in human keratinocytes is regulated by negative and positive promoter regions: V.P. Ramirez, et al.; Cell Stress Chaperones 20, 95 (2015), Abstract;
Centrosome-intrinsic mechanisms modulate centrosome integrity during fever: A. Vertii, et al.; Mol. Biol. Cell 26, 3451 (2015), Application(s): Cell culture , Abstract; Full Text
Combination of PEI-Mn0.5Zn0.5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma: Q. Tang, et al.; Int. J. Nanomedicine 10, 7129 (2015), Abstract; Full Text
Cross-Omics Comparison of Stress Responses in Mesothelial Cells Exposed to Heat- versus Filter-Sterilized Peritoneal Dialysis Fluids: K. Katochwill, et al.; Biomed Res. Int. 2015, Article ID 628158 (2015), Application(s): Western Blot, Abstract; Full Text
Differential expression patterns among heat-shock protein genes and thermal responses in the whitefly Bemisia tabaci(MEAM 1): F. Diaz, et al.; J. Therm. Biol. 52, 199 (2015), Application(s): Western Blot, Abstract;
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
Dynamic m6A mRNA methylation directs translational control of heat shock response: J. Zhou, et al.; Nature 526, 591 (2015), Application(s): Immunoblotting, Abstract;
Genomic and proteomic investigation of preeclampsia: H. Sahin, et al.; Exp. Ther. Med. 10, 711 (2015), Application(s): Western Blot, Abstract; Full Text
Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE: K. Saito, et al.; Eur. J. Immunol. 45, 2028 (2015), Abstract; Full Text
Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines: F. Clemente-Vicario, et al.; PLoS One 10, e0142007 (2015), Application(s): Immunoblot analyses, Abstract; Full Text
Linking HSP90 target occupancy to HSP70 induction and efficacy in mouse brain: K. Thirstrup, et al.; Pharmacol. Res. 104, 197 (2015), Application(s): Immunoblot analysis using mouse cell and tissue homogenates, Abstract;
Mechanism of Intracellular Communication During Yersinia Pestis Infection: F. Alem; (2015), (master thesis), Application(s): Cytoskeletal Rearrangement, Cell Migration, and Vesicle Trafficking, Abstract; Full Text
Merging colloidal nanoplasmonics and surface plasmon resonance spectroscopy for enhanced profiling of multiple myeloma-derived exosomes: D.N. Giuseppe, et al.; Biosens. Bioelectron. 77, 518 (2015), Application(s): Western Blot, Abstract;
NZ28-induced inhibition of HSF1, SP1 and NF-κB triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells: D. Schilling, et al.; Cancer Immunol. Immunother. 64, 599 (2015), Abstract; Full Text
Selective astrocytic endothelin-1 overexpression contributes to dementia associated with ischemic stroke by exaggerating astrocyte-derived amyloid secretion: V. K. Hung, et al.; J. Cereb. Blood Flow Metab. 35, 1687 (2015), Application(s): Western Blot, Abstract;
Sensitizing tumor cells to radiation by targeting the heat shock response: D. Schilling, et al.; Cancer Lett. 360, 294 (2015), Application(s): Western Blotting, Abstract;
Short-Wave Diathermy Pretreatment and Inflammatory Myokine Response After High-Intensity Eccentric Exercise: J. P. Vardiman, et al.; J. Athl. Train. 50, 612 (2015), Application(s): Cell Culture, Abstract; Full Text
Structure-Activity Relationship in a Purine-Scaffold Compound Series with Selectivity for the Endoplasmic Reticulum Hsp90 Paralog Grp94: H. J. Patel, et al.; J. Med. Chem. 58, 3922 (2015), Application(s): Western Blot, Abstract; Full Text
Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia: D. Brea, et al.; Sci. Rep. 5, 12030 (2015), Application(s): Immunohistochemistry, Abstract; Full Text
Thiopental protects human neuroblastoma cells from apoptotic cell death - Potential role of heat shock protein 70: M. Roesslein, et al.; Life Sci. 139, 40 (2015), Application(s): Gel electrophoresis, Western Blotting, Abstract;
TNIP1 reduction of HSPA6 gene expression occurs in promoter regions lacking binding sites for known TNIP1-repressed transcription factors: V.P. Ramirez, et al.; Gene 555, 430 (2015), Application(s): Western blot using human keratinocyte HaCaT cell lysates, Abstract;
Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats: R.R. Ahmed, et al.; Biol. Res. 48, 54 (2015), Application(s): Immunohistochemistry, Abstract; Full Text
Effects of vitamin C and E supplementation on endogenous antioxidant systems and heat shock proteins in response to endurance training: K.T. Cumming, et al.; Physiol. Rep. 2, e12142 (2014), Application(s): Western blot using human muscle tissue lysates, Abstract; Full Text
HSF1 deficiency and impaired HSP90-dependent protein folding are hallmarks of aneuploid human cells: N. Donnelly, et al.; EMBO J. 33, 2374 (2014), Abstract; Full Text
Mutations in the substrate binding site of human heat-shock protein 70 indicate specific interaction with HLA-DR outside the peptide binding groove: K.M. Rohrer, et al.; Immunology 142, 237 (2014), Abstract; Full Text
Determining the Contributions of Caspase-2, Caspase-8 and Effector Caspases to Intracellular VDVADase Activities during Apoptosis Initiation and Execution: M.E. Delgado, et al.; Biochim. Biophys. Acta 1833, 2279 (2013), Application(s): WB using human cell lysates, Abstract;
In vivo theranostics at the peri-infarct region in cerebral ischemia: J. Agulla, et al.; Theranostics 4, 90 (2013), Application(s): IHC, WB of rat brain, Abstract; Full Text
OLA1 protects cells in heat shock by stabilizing HSP70: R.F. Mao, et al.; Cell Death Dis. 14, e491 (2013), Abstract; Full Text
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text
Daily quadratic trend in basal monocyte expressed HSP72 in healthy human subjects: L. Taylor, et al.; Amino Acids 38, 1483 (2010), Abstract;
Measuring the pharmacodynamic effects of a novel Hsp90 inhibitor on HER2/neu expression in mice using Zr-DFO-trastuzumab: J.P. Holland, et al.; PLoS One 5, e8859 (2010), Abstract; Full Text
Methylglyoxal alters the function and stability of critical components of the protein quality control: C. F. Bento, et al.; PLoS One 5, e13007 (2010), Application(s): WB using human cell lysates, Abstract; Full Text
The effect of acute hypoxia on heat shock protein 72 expression and oxidative stress in vivo: L. Taylor, et al.; Eur. J. Appl. Physiol. 109, 849 (2010), Abstract;
Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat Shock Proteins and Viral Tumor Antigens: P.P. Li, et al.; J. Virol. 83, 37 (2009), Application(s): WB, IP, IHC using monkey cell lysates, Abstract;
Hsp90 charged-linker truncation reverses the functional consequences of weakened hydrophobic contacts in the N domain: S. Tsutsumi, et al. ; Nat. Struct. Mol. Biol. 16, 1141 (2009), Application(s): WB, IP using mouse cell lysates, Abstract;
Hypertonic saline increases gammadeltaT cell-mediated killing of activated neutrophils: M.I. Hirsh, et al.; Crit. Care Med. 36, 3220 (2008), Abstract;
Induction of Cellular Stress Overcomes the Requirement of Herpes Simplex Virus Type 1 for Immediate-Early Protein ICP0 and Reactivates Expression from Quiescent Viral Genomes: C. Preston, et al. ; J. Virol. 82, 11775 (2008), Application(s): WB using human cell lysates, Abstract;
Neurodegeneration and cellular stress in the retina and optic nerve in rat cerebral ischemia and hypoperfusion models: G. Kalesnykas, et al. ; Neuroscience 155, 937 (2008), Application(s): IHC using rat tissue, Abstract;
Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation: P. Jaakkola, et al. ; Mol. Biol. Cell 19, 2231 (2008), Application(s): ICC using human tissue culture, Abstract;
Exogenous androgen treatment delays the stress response following hamster facial nerve injury: K. Jones, et al. ; J. Neuroendocrinol. 19, 383 (2007), Application(s): IHC using hamster tissue, Abstract;
fMLP induces Hsp27 expression, attenuates NF-κB activation, and confers intestinal epithelial cell protection: E. Chang, et al. ; Am. J. Physiol. Gastrointest. Liver Physiol. 292, G1070 (2007), Application(s): WB using human tissue, Abstract;
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R844 (2007), Application(s): WB using human vastus lateralis muscle tissue, Abstract; Full Text
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: S. Chen & I.R. Brown; Cell Stress Chaperones 12, 51 (2007), Application(s): WB using rat tissue, Abstract;
Persistent sub-lethal chlorine exposure augments temperature induced immunosuppression in Cyprinus carpio advanced fingerlings: A. Pal, et al. ; Fish Shellfish Immunol. 22, 547 (2007), Application(s): WB using fish samples, Abstract;
Abdominal temperature induces region-specific p53-independent apoptosis in the cauda epididymidis of the mouse: M. Jara, et al.; Biol. Reprod. 67, 1189 (2002), Application(s): WB using mouse tissue homogenates, Abstract; Full Text
Chemical preconditioning with 3-nitropropionic acid: lack of induction of neuronal tolerance in gerbil hippocampus subjected to transient forebrain ischemia: P. Garnier, et al.; Brain Res. Bull. 58, 33 (2002), Abstract;
Xenopus small heat shock proteins, Hsp30C and Hsp30D, maintain heat- and chemically denatured luciferase in a folding-competent state: R. Abdulle, et al.; Cell Stress Chaperones 7, 6 (2002), Abstract;
Presence of both constitutive and inducible forms of heat shock protein 70 in the cerebral cortex and hippocampal synapses: I.S. Moon, et al.; Cereb. Cortex 11, 238 (2001), Abstract;
Suppression of heat shock protein-70 by ceramide in heat shock-induced HL-60 cell apoptosis: T. Kondo, et al.; J. Biol. Chem. 275, 8872 (2000), Abstract;
The ubiquitin-related BAG-1 provides a link between the molecular chaperones Hsc70/Hsp70 and the proteasome: J. Luders, et al.; J. Biol. Chem. 275, 4613 (2000), Abstract;
Two distinct domains in hsc70 are essential for the interaction with the synaptic vesicle cysteine string protein: B. Stahl, et al.; Eur. J. Cell Biol. 78, 375 (1999), Abstract;
Chaperone suppression of aggregation and altered subcellular proteasome localization imply protein misfolding in SCA1: C.J. Cummings, et al.; Nat. Genet. 19, 148 (1998), Abstract;
Detection of heat shock protein 70 (Hsp70) and anti-Hsp70 antibodies in the serum of normal individuals: A.G. Pockley, et al.; Immunol. Invest. 27, 367 (1998), Abstract;
Differential effects of axotomy on the in vivo synthesis of the stress-inducible and constitutive 70-kDa heat-shock proteins in rat dorsal root ganglia: B. Tedeschi & R.P. Ciavarra; Brain Res. Mol. Brain Res. 45, 199 (1997), Abstract;
A hitchhiker's guide to the human Hsp70 family: M. Tavaria, et al.; Cell Stress Chaperones 1, 23 (1996), Abstract;
Structural analysis of substrate binding by the molecular chaperone DnaK: X. Zhu, et al.; Science 272, 1606 (1996), Abstract;
Stress protein induction in skeletal muscle: comparison of laboratory models to naturally occurring hypertrophy: J.L. Kilgore, et al.; J. Appl. Physiol. 76, 598 (1994), Abstract;
Cellular and biochemical events in mammalian cells during and after recovery from physiological stress: W.J. Welch & J.P. Suhan; J. Cell Biol. 103, 2035 (1986), Abstract;
A molecular chaperone that assists in the folding of emerging polypeptides and the refolding of denatured proteins.
Produced in E. coli., ≥95% (SDS-PAGE; Western blot), ELISA, IHC, WB, Activity assay, Antigen microarray, Apoptosis assay, in vitro Assay | Print as PDF
