| ADI-SPA-800PE-E | 100 µg | 407.00 USD |  |
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| Alternative Name: | HspB1, Heat shock protein 27 |
| Clone: | G3.1 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Native human Hsp27. |
| UniProt ID: | P04792 |
| GenBank ID: | L39370 |
| Source: | Purified from mouse ascites. |
| Species reactivity: | Human, Mouse, Rat Bovine, Fish, Monkey |
| Applications: | Flow Cytometry |
| Recommended Dilutions/Conditions: | Flow Cytometry (10µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Purity Detail: | Protein G affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
| Handling: | Avoid vigorous centrifugation and vortexing. Do not freeze. Protect from light. |
| Shipping: | Blue Ice Not Frozen |
| Long Term Storage: | +4°C |
| Scientific Background: | Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer. |
| Regulatory Status: | RUO - Research Use Only |
Mouse IgG1 isotype control, monoclonal antibody (MOPC-21) (PE conjugate) |
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| Purified from ascites., Flow Cytometry | Print as PDF | ||||||
| ADI-SAB-600PE-D | 50 µg | 256.00 USD | |
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| ADI-SAB-600PE-F | 200 µg | 528.00 USD | |
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