Product Details
| Alternative Name: | Superoxide Dismutase (Cu-Zn), Superoxide dismutase 1, SOD1 |
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| Host: | Rabbit |
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| Immunogen: | Native human Cu/Zn SOD. |
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| UniProt ID: | P00441 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Clam
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| Applications: | IF, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Immunofluorescence (1:50-1:200)
Immunohistochemistry (paraffin sections, 1:50)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~23kDa (human) or ~19kDa (mouse and rat) by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | SOD (Superoxide dismutase) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells, they are: EC SOD (extracellular SOD), Cu/Zn SOD (copper and zinc-containing SOD) and Mn SOD (manganese-containing SOD). |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Cu/Zn SOD, pAb (Prod. No. ADI-SOD-100): Lane 1: Rat liver tissue lysate, Lane 2: Mouse liver tissue lysate, Lane 3: HeLa cell lysate (Prod. No. ADI-LYC-HL100).
Immunohistochemistry analysis of human heart tissue stained with Cu/Zn SOD, pAb at 10µg/ml.
Immunofluorescent analysis (confocal) staining of HeLa cells using Cu/Zn SOD pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.
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Product Literature References
Identification of HSPB8 modulators counteracting misfolded protein accumulation in neurodegenerative diseases: M. Chierichetti, et al.; Life Sci.
322, 121323 (2023),
Abstract;
microRNA-146a modulates behavioural activity, neuroinflammation, and oxidative stress in adult mice: W. Zhao, et al.; Mol. Cell. Neurosci.
124, 103820 (2023),
Abstract;
Neuronal deletion of MnSOD in mice leads to demyelination, inflammation and progressive paralysis that mimics phenotypes associated with progressive multiple sclerosis: S. Bhaskaran, et al.; Redox Biol.
59, 102550 (2023),
Abstract;
Altered SOD1 maturation and post-translational modification in amyotrophic lateral sclerosis spinal cord: B.G. Trist, et al.; Brain
145, 3108 (2022),
Abstract;
Cellular analysis of SOD1 protein-aggregation propensity and toxicity: a case of ALS with slow progression harboring homozygous SOD1-D92G mutation: M. Sawamura, et al.; Sci. Rep.
12, 12636 (2022),
Abstract;
Cerium dioxide nanoparticles modulate antioxidant defences and change vascular response in the human saphenous vein: S.G. Ojeda, et al.; Free Radic. Biol. Med.
193, 694 (2022),
Abstract;
Redox stress and metal dys-homeostasis appear as hallmarks of early prion disease pathogenesis in mice: J.G. Spiers, et al.; Free Radic Biol. Med.
192, 182 (2022),
Abstract;
Potential influence of prenatal 2.45 GHz radiofrequency electromagnetic field exposure on Wistar albino rat testis: V. Almášiová, et al.; Histol. Histopathol.
18, 331 (2021),
Abstract;
Transgenic expression of SOD1 specifically in neurons of Sod1 deficient mice prevents defects in muscle mitochondrial function and calcium handling: Y. Su, et al.; Free Radic. Biol. Med.
165, 299 (2021),
Abstract;
Effect of gomisin A on osteoblast differentiation in high glucose-mediated oxidative stress: J.S. Takanche, et al.; Phytomedicine
66, 153107 (2020),
Abstract;
Repetitively Hypoxic Preconditioning Attenuates Ischemia/Reperfusion-Induced Liver Dysfunction Through Upregulation of Hypoxia-Induced factor-1 Alpha-Dependent Mitochondrial Bcl-xl in Rat: P. Chou, et al.; Chin. J. Physiol.
63, 68 (2020),
Abstract;
Full Text
An endogenous peptide marker differentiates SOD1 stability and facilitates pharmacodynamic monitoring in SOD1 amyotrophic lateral sclerosis: I. Gertsman, et al.; JCI Insight
4, 122768 (2019),
Abstract;
Full Text
Antihypertensive Potential of Coenzyme Q10 via Free Radical Scavenging and Enhanced Akt-nNOS Signaling in the Nucleus Tractus Solitarii in Rats: H.H. Chen, et al.; Mol. Nutr. Food Res.
63, e1801042 (2019),
Abstract;
Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells: E. Pokrishevsky, et al.; Methods Mol. Biol.
1873, 213 (2019),
Application(s): Western Blot of cell lysates,
Abstract;
Inhibition of p300/CBP-Associated Factor Attenuates Renal Tubulointerstitial Fibrosis through Modulation of NF-kB and Nrf2: S. Chung, et al.; Int. J. Mol. Sci.
20, 1554 (2019),
Abstract;
Full Text
Interval running training improves age‐related skeletal muscle wasting and bone loss: Experiments with ovariectomized rats: J.S. Kim, et al.; Exp. Physiol.
104, 691 (2019),
Abstract;
Late life maintenance and enhancement of functional exercise capacity in low and high responding rats after low intensity treadmill training: L.A. Brown, et al.; Exp. Gerontol.
125, 110657 (2019),
Abstract;
Nrf2 signaling links ER oxidative protein folding and calcium homeostasis in health and disease: V. Granatiero, et al.; Life Sci. Alliance
2, e201900563 (2019),
Abstract;
Full Text
Suppression of the gut microbiome ameliorates age‐related arterial dysfunction and oxidative stress in mice: V.E. Brunt, et al.; J. Physiol.
597, 2361 (2019),
Abstract;
Tryptophan 32 mediates SOD1 toxicity in a in vivo motor neuron model of ALS and is a promising target for small molecule therapeutics: M.G. DuVal, et al.; Neurobiol. Dis.
124, 297 (2019),
Abstract;
A small-molecule inhibitor of SOD1-Derlin-1 interaction ameliorates pathology in an ALS mouse model: N. Tsuburaya, et al.; Nat. Commun.
9, 2668 (2018),
Abstract;
Full Text
Aggregated SOD1 causes selective death of cultured human motor neurons: C. Benkler, et al.; Sci. Rep.
8, 16393 (2018),
Abstract;
Full Text
Elongator subunit 3 (ELP3) modifies ALS through tRNA modification: A. Bento-Abreu, et al.; Hum. Mol. Genet.
27, 1276 (2018),
Abstract;
Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function: E.M. Hock, et al.; Cell Rep.
24, 987 (2018),
Abstract;
Redox responses are preserved across muscle fibres with differential susceptibility to aging: N.T. Smith, et al.; J. Proteomics
177, 112 (2018),
Abstract;
Full Text
Selective eradication of cancer displaying hyperactive Akt by exploiting the 3 metabolic consequences of Akt activation: V. Nogueira, et al.; eLife
7, e32213 (2018),
Abstract;
Full Text
Angiotensin receptor blockade improves cardiac mitochondrial activity in response to an acute glucose load in obese insulin resistant rats: M. Thorwald, et al.; Redox Biol.
14, 371 (2017),
Abstract;
Full Text
eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet: W.G. Choi, et al.; Nutr.
14, 48 (2017),
Abstract;
Full Text
Inhibition of retrograde transport modulates misfolded protein accumulation and clearance in motoneuron diseases: R. Cristofani, et al.; Autophagy
13, 1280 (2017),
Abstract;
Full Text
Localization of a mutant SOD1 protein in E40K-heterozygous dogs: Implications for non-cell-autonomous pathogenesis of degenerative myelopathy: Y. Kobatake, et al.; J. Neurol. Sci.
372, 369 (2017),
Application(s): Immunocytochemistry of canine SOD1-transfected cells, human embryonic kidney (HEK) 293A cells,
Abstract;
Lysyl oxidase overexpression accelerates cardiac remodeling and aggravates angiotensin II–induced hypertrophy: M. Galan, et al.; FASEB J.
31, 3787 (2017),
Abstract;
Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice: S.A. Bloomer, et al.; Lipids Health Dis.
16, 243 (2017),
Abstract;
Full Text
A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression: M. Kawaguchi, et al.; BMC Cancer
16, 805 (2016),
Application(s): Western blot, nuclear and cytoplasmic protein extracts,
Abstract;
Full Text
Apollo Lunar Astronauts Show Higher Cardiovascular Disease Mortality: Possible Deep Space Radiation Effects on the Vascular Endothelium: M.D. Delp, et al.; Sci. Rep.
6, 29901 (2016),
Application(s): Immunoblot Analysis,
Abstract;
Full Text
Cerebrovascular endothelial dysfunction induced by mercury exposure at low concentrations: G.A. Wiggers, et al.; Neurotoxicology
53, 282 (2016),
Application(s): Western blot,
Abstract;
Exposure of Female Rats to an Environmentally-Relevant Mixture of Brominated Flame Retardants Targets the Ovary, Affecting Folliculogenesis and Steroidogenesis: P.L. Lefevre, et al. ; Biol. Reprod.
94, 9 (2016),
Application(s): Western Blot,
Abstract;
Full Text
Palmitoylethanolamide protects mice against 6-OHDA-induced neurotoxicity and endoplasmic reticulum stress: In vivo and in vitro evidence: C. Avagliano, et al.; Pharmacol. Res.
113, 276 (2016),
Abstract;
Photo-protective effect of sargachromenol against UVB radiation-induced damage through modulating cellular antioxidant systems and apoptosis in human keratinocytes: P.M. Fernando, et al.; Environ. Toxicol. Pharmacol.
43, 112 (2016),
Application(s): Western blot,
Abstract;
The effect of R-(-)-deprenyl administration on antioxidant enzymes in rat testis: F. Horvathova, et al. ; Eur. J. Pharmacol.
788, 21 (2016),
Application(s): Immunohistochemistry,
Abstract;
A systematic immunoprecipitation approach reinforces the concept of common conformational alterations in amyotrophic lateral sclerosis-linked SOD1 mutants: T. Fujisawa, et al. ; Neurobiol. Dis.
82, 478 (2015),
Application(s): Immunoblotting,
Abstract;
Accumulation and aggregate formation of mutant superoxide dismutase 1 in canine degenerative myelopathy: S. Nakamae, et al.; Neurosci.
303, 229 (2015),
Application(s): Western Blot,
Abstract;
Changes of the Cytoplasmic Proteome in Response to Alcoholic Hepatotoxicity in Rats: D.H. Kim, et al. ; Int. J. Mol. Sci.
16, 18664 (2015),
Application(s): Immunoblotting ,
Abstract;
Full Text
Correlations between Cu, Zn- Superoxide dismutase and Macrophages or MUC5AC in Human Eosinophilic Chronic Rhinosinusitis: T. Kusunoki, et al.; J. Otol. Rhinol.
S1, 1 (2015),
Application(s): Immunostaining,
Full Text
Effects of Exercise and Alpha-Lipoic Acid Supplementation on Brain Tissue Protection in Experimental Diabetes: Z. Pündük; (2015), (PhD thesis),
Application(s): Western assay,
Full Text
Respiratory syncytial virus infection downregulates antioxidant enzyme expression by TRIGGERING deacetylation-proteasomal degradation of NRF2: N. Komaravelli, et al.; Free Radic. Biol. Med.
88, 391 (2015),
Application(s): Western Blot,
Abstract;
Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions: J.A. Collins, et al.; Free Radic. Biol. Med.
86, 57 (2015),
Application(s): Western Blot,
Abstract;
The beneficial effects of exercise on cartilage are lost in mice with reduced levels of ECSOD in tissues: K.M. Pate, et al.; J. Appl. Physiol. (1985)
118, 760 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
Versican Localizes to the Nucleus in Proliferating Mesenchymal Cells: J.M. Carthy, et al.; Cardiovasc. Pathol.
24, 368 (2015),
Application(s): Western Blot,
Abstract;
α-synuclein interacts with SOD1 and promotes its oligomerization: A.M. Helferich, et al.; Mol. Neurodegener.
10, 66 (2015),
Application(s): Western Blot and Co-Immunoprecipitation of H4 human neuroglioma cells; IHC of mouse brain tissue; ,
Abstract;
Full Text
Sod1 loss induces intrinsic superoxide accumulation leading to p53-mediated growth arrest and apoptosis: K. Watanabe, et al.; Int. J. Mol. Sci.
14, 10998 (2013),
Application(s): Western blot,
Abstract;
Full Text
Activation of PPARδ prevents endothelial dysfunction induced by overexpression of amyloid-β precursor protein: L.V. d'Uscio, et al.; Cardiovasc. Res.
96, 504 (2012),
Application(s): Western Blot of mouse aorta lysates,
Abstract;
Full Text
Erythropoietin attenuated vascular dysfunction and inflammation by inhibiting NADPH oxidase-derived superoxide production in nitric oxide synthase-inhibited hypertensive rat aorta: H. Toba, et al.; Eur. J. Pharmacol.
691, 190 (2012),
Application(s): Western Blot of rat aorta lysates,
Abstract;
Huntingtin fragments and SOD1 mutants form soluble oligomers in the cell: Y.N. Park, et al.; PLoS One
7, e40329 (2012),
Application(s): WB using N2A and HeLA cells,
Abstract;
Full Text
A novel mouse model with impaired dynein/dynactin function develops amyotrophic lateral sclerosis (ALS)-like features in motor neurons and improves lifespan in SOD1-ALS mice: D. Jaarsma, et al. ; Hum. Mol. Genet.
17, 2849 (2008),
Application(s): WB using mouse tissue,
Abstract;
Effect of exposure to benzo[a]pyrene on SODs, CYP1A1/1A2- and CYP2E1 immunopositive proteins in the blood clam Scapharca inaequivalvis: M. Monari, et al. ; Mar. Environ. Res.
63, 200 (2007),
Application(s): WB using clam tissue,
Abstract;
Aging induces muscle-specific impairment of endothelium-dependent dilation in skeletal muscle feed arteries: M.H. Laughlin, et al. ; J. Appl. Physiol.
93, 1685 (2002),
Application(s): WB using rat samples,
Abstract;
Dorfin ubiquitylates mutant SOD1 and prevents mutant SOD1-mediated neurotoxicity: G. Sobue, et al. ; J. Biol. Chem.
277, 36793 (2002),
Application(s): IHC using human samples,
Abstract;
Effect of mutant alpha-synuclein on dopamine homeostasis in a new human mesencephalic cell line: P. Brundin, et al. ; J. Biol. Chem.
277, 38884 (2002),
Application(s): WB using human samples,
Abstract;
Mutant Cu/Zn-superoxide dismutase proteins have altered solubility and interact with heat shock/stress proteins in models of amyotrophic lateral sclerosis: H.D. Durham, et al. ; J. Biol. Chem.
276, 12791 (2001),
Application(s): IP using human samples,
Abstract;
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