| ADI-LYT-RB100-F | 200 µg | 225.00 USD |  |
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| Do you need bulk/larger quantities? | |||||
| Recommended Dilutions/Conditions: | Western Blot (20µg/lane) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Source: | Rat brain extract |
| Formulation: | Liquid. In SDS-PAGE sample buffer, pH 6.8, containing 40mM TRIS-HCl, 1% SDS, 50mM DTT, 7.5% glycerol, and 0.003% bromophenol blue. |
| Applications: | WB |
| Application Notes: | Western blot control. |
| Shipping: | Dry Ice |
| Long Term Storage: | -80°C |
| Handling: | Avoid freeze/thaw cycles. |
| Scientific Background: | Brains from male Sprague Dawley rats were suspended in M-PER, Mammalian Protein Extraction Reagent, with protease inhibitor cocktail and homogenized using a Dounce tissue grinder. The tissue lysate was adjusted to 2mg/mL in SDS PAGE sample buffer (40 mM Tris-HCl pH 6.8, 1% SDS, 50 mM DTT, 7.5% glycerol, 0.003% bromophenol blue) and heated for 10 min at 70°C. |
| Regulatory Status: | RUO - Research Use Only |
