Product Details
| Alternative Name: | Nuclear factor erythroid 2-related factor 2 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide derived from the sequence of human Nrf2 (residues 40-60) with a cysteine residue added and coupled to KLH. |
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| UniProt ID: | Q16236 |
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| Gene/Protein Identifier: | NP_006155 (RefSeq) |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Dog, Guinea pig, Hamster
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| Applications: | WB
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor, which regulates the expression of many detoxification and antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar subfamily of basic leucine zipper (CNC-bZIP) transcription factors. The NF-E2 related family comprises of Nrf1 (TCFII), Nrf2, and Nrf3 and these proteins heterodimerize with Maf proteins to bind to Maf recognition element (MARE). Under normal conditions, Nrf2 is inactive and localized in the cytoplasm through its association with Keap1. When cells are exposed to electrophiles or oxidative stress generating agents cytoplasm localization of Nrf2 is inactivated and Nrf2 translocates into the nucleus and binds to specific DNA sequence elements known as NF-E2 binding site, Maf recognition element (MARE), stress response element or anti-oxidant response element (ARE). Studies have investigated the role of Nrf2 in urinary bladder carcinogenesis, pulmonary fibrosis, and smoke-induced emphysema. A study reported Nrf2-mediated ARE transcription to be a likely target of preventative therapy in neurodegenerative disorders such as Huntington's disease. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Nrf2: Lane 1: MW Marker, Lane 2: Rat Liver Microsomes, Lane 3: Mouse Liver Microsomes, Lane 4: Human Liver Microsomes, Lane 5: Canine Liver Microsomes, Lane 6: HeLa Cell Lysate.
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Product Literature References
A Mitochondrion-Targeting Protein (B2) Primes ROS/Nrf2-Mediated Stress Signals, Triggering Apoptosis and Necroptosis in Lung Cancer: H.W. Chiu, et al.; Biomedicines
11, 186 (2023),
Abstract;
Increased oxidative stress contributes to enhance brain amyloidogenesis and blunts energy metabolism in sucrose-fed rat: effect of AMPK activation: L. Camacho-Castillo, et al.; Sci. Rep.
11, 19547 (2021),
Abstract;
The Anti-Diabetic Drug Metformin Rescues Aberrant Mitochondrial Activity and Restrains Oxidative Stress in a Female Mouse Model of Rett Syndrome: I. Zuliani, et al.; J. Clin. Med.
9, 1669 (2020),
Abstract;
Sulforaphane Cannot Protect Human Fibroblasts From Repeated, Short and Sublethal Treatments with Hydrogen Peroxide: M.C. Lionetti, et al.; Int. J. Environ. Res. Public Health
16, E657 (2019),
Application(s): Western blot using human primary dermal fibroblast (hSDF) lysates,
Abstract;
Full Text
α-Ecdysone suppresses inflammatory responses via the Nrf2 pathway in lipopolysaccharide-stimulated RAW 264.7 cells: M. Bhardwaj, et al.; Int. Immunopharmacol.
73, 405 (2019),
Abstract;
Autophagy Deficiency Diminishes Indomethacin-Induced Intestinal Epithelial Cell Damage through the Activation of ERK/Nrf2/HO-1 Pathway: S. Harada, et al.; J. Pharmacol. Exp. Ther.
355, 353 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
Dimethyl Fumarate Confers Neuroprotection by Casein Kinase 2 Mediated Phosphorylation of Nrf2 in Murine Intracerebral Hemorrhage: L.O. Iniaghe, et al.; Neurobiol. Dis.
82, 349 (2015),
Application(s): Western Blot,
Abstract;
Oxidative stress by layered double hydroxide nanoparticles via an SFK-JNK and p38-NF-κB signaling pathway mediates induction of interleukin-6 and interleukin-8 in human lung epithelial cells: S.J. Choi, et al.; Int. J. Nanomedicine
10, 3217 (2015),
Application(s): Western Blot,
Abstract;
Full Text
DHA sensitizes FaO cells to tert-BHP-induced oxidative effects. Protective role of EGCG: A. Fernandez-Iglesias, et al.; Food Chem. Toxicol.
62, 750 (2013),
Abstract;
