Product Details
Alternative Name: | CSNK, CK |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to a portion of human CK2α (casein kinase 2α). The sequence is completely conserved in mouse, rat, rabbit, chicken, Xenopus, zebra fish, Drosophila and C. elegans. |
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UniProt ID: | P68400 (CK2 alpha), P19784 (CK2 alpha') |
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GenBank ID: | X70251 (CK2 alpha), M55268 (CK2 alpha') |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human, Mouse, Rat Bovine, Chinook salmon, Drosophila, Guinea pig, Monkey, Rabbit, Sheep
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Applications: | IF, IHC, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (1.7-8.5µg/ml) Western Blot (4µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects band of ~41kDa and ~45kDa by Western blot. An additional band with an apparent molecular mass of ~38 kDa is detected in brain and heart tissue extracts. |
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Purity Detail: | Immunoaffinity purified. |
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Formulation: | Liquid. In borate buffered saline, pH 8.2, containing 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, and 5mM EDTA (disodium salt). |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The casein kinases are serine/threonine protein kinases involved in the regulation of multiple cell signaling pathways. There are at least seven identified mammalian isoforms in the casein kinase I (CKI) family, which are monomeric and function in cell growth and the replication and repair of DNA. Casein kinase II (CK2) is a ubiquitous tetrameric holoenzyme comprised of two catalytic alpha subunits and two regulatory beta subunits. CK2 enzymes are known to regulate metabolic signal transduction, transcription, and translation. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of mouse spleen tissue extract, probed with Casein Kinase 2 alpha polyclonal antibody (Prod. No. ADI-KAP-ST010).
Proliferating human Caco-2 cells stained with Casein Kinase 2 alpha polyclonal antibody (Prod. No. ADI-KAP-ST010).
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Product Literature References
Dysregulation of integrin-linked kinase (ILK) signaling in colonic polyposis: B. Salh, et al. ; Oncogene
20, 6250 (2003),
Application(s): WB using human samples,
Abstract;
A role for casein kinase 2alpha in the Drosophila circadian clock: R. Allada, et al. ; Nature
420, 816 (2002),
Application(s): IHC using drosophila samples,
Abstract;
Functional interaction of protein kinase CK2 and c-Myc in lymphomagenesis: D.C. Seldin, et al. ; Oncogene
21, 5280 (2002),
Application(s): WB using mouse samples,
Abstract;
Heat-shock protein90 complexes in resting and thrombin-activated platelets: R.L. Gear, et al. ; Biochem. Biophys. Res. Commun.
297, 129 (2002),
Application(s): WB using human samples,
Abstract;
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