Product Details
| Alternative Name: | Protein kinase G |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to a portion of human PKG. |
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| UniProt ID: | Q13976 |
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| GenBank ID: | D45864 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Porcine
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~75kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Cyclic GMP-dependent protein kinases (PKGs or cGKs) are classified into two types, PKGI and PKGII. Studies have shown that PKGs are highly homologous to PKAs; phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility, and platelet aggregation. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis: Lane 1: MW marker, Lane 2: Mouse Brain Tissue Extract, Lane 3: Rat Brain Tissue Extract, Lane 4: EKS4 Cell Lysate; Lane 5: HS67 Cell Lysate probed with PKG pAb.
Immunohistochemistry analysis of human prostate tissue stained with PKG, pAb at 10µg/ml.
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Product Literature References
Detecting Validated Intracellular ROS Generation with 18F-dihydroethidine-Based PET: E.C.T. Waters, et al.; Mol. Imaging Biol.
24, 377 (2022),
Abstract;
LC-MS/MS Validation and Quantification of Cyanotoxins in Algal Food Supplements from the Belgium Market and Their Molecular Origins: F.A. Beñaldo, et al.; Front. Physiol.
13, 864010 (2022),
Abstract;
Alteration of the soluble guanylate cyclase system in coronary arteries of high cholesterol diet-fed rabbits: M. Tawa, et al.; Pharmacol. Res. Perspect.
9, e00838 (2021),
Abstract;
Ca2+ signals critical for egress and gametogenesis in malaria parasites depend on a multipass membrane protein that interacts with PKG: A.C. Balestra, et al.; Sci. Adv.
7, 5396 (2021),
Abstract;
Full Text
Hydrogen peroxide signaling via its transformation to a stereospecific alkyl hydroperoxide that escapes reductive inactivation: R.F. Queiroz, et al.; Nat. Commun.
12, 6626 (2021),
Abstract;
Implications of Iron Deficiency in STEMI Patients and in a Murine Model of Myocardial Infarction: J. Inserte, et al.; JACC Basic Transl. Sci.
6, 567 (2021),
Abstract;
"A Step and a Ceiling": mechanical properties of Ca2+ spark vasoregulation in resistance arteries by pressure-induced oxidative activation of PKG: V. Csato, et al.; Physiol. Rep.
7, e14260 (2019),
Abstract;
Full Text
Singlet molecular oxygen regulates vascular tone and blood pressure in inflammation: C.P. Stanley, et al.; Nature
566, 548 (2019),
Abstract;
Age related differences in responsiveness to sildenafil and tamsulosin are due to myogenic smooth muscle tone in the human prostate: S.N. Lee, et al.; Sci. Rep.
7, 10150 (2017),
Application(s): Immunohistochemistry,
Abstract;
Full Text
Examining a role for PKG Iα oxidation in the pathogenesis of cardiovascular dysfunction during diet-induced obesity: O. Rudyk, et al.; Free Radic. Biol. Med.
110, 390 (2017),
Application(s): Western blot,
Abstract;
Full Text
Oxidant sensor in the cGMP-binding pocket of PKGIα regulates nitroxyl-mediated kinase activity: S. Donzelli, et al.; Sci. Rep.
7, 9938 (2017),
Application(s): Western blot,
Abstract;
Full Text
The augmentation of BK channel activity by nitric oxide signaling in rat cerebral arteries involves co-localized regulatory elements: B.D. Kyle, et al.; J. Cereb. Blood Flow Metab.
37, 3759 (2017),
Abstract;
Full Text
Type 5 phosphodiesterase regulates glioblastoma multiforme aggressiveness and clinical outcome: V. Cesarini, et al.; Oncotarget
8, 13223 (2017),
Application(s): Immunohistochemistry,
Abstract;
Full Text
Disulfide-activated protein kinase G Iα regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response: J. Scotcher, et al.; Nat. Commun.
7, 13187 (2016),
Application(s): Western blot,
Abstract;
Full Text
Disulfide-activated protein kinase G Iα regulates cardiac diastolic relaxation and fine-tunes the Frank–Starling response: J. Scother, et al.; Nat. Commun.
7, 13187 (2016),
Abstract;
Full Text
Phosphodiesterase 5 inhibition limits doxorubicin-induced heart failure by attenuating protein kinase G Iα oxidation: O. Prysyazhna, et al.; J. Biol. Chem.
291, 17427 (2016),
Application(s): Western blot,
Abstract;
Full Text
cCMP causes caspase-dependent apoptosis in mouse lymphoma cell lines: S. Wolter, et al.; Biochem. Pharmacol.
98, 119 (2015),
Application(s): Western blot,
Abstract;
Crystal Structures of the Carboxyl cGMP Binding Domain of the Plasmodium falciparum cGMP-dependent Protein Kinase Reveal a Novel Capping Triad Crucial for Merozoite Egress: J.J. Kim, et al.; PLoS Pathog.
11, e1004639 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus: C. Mátyás, et al.; Cardiovasc. Diabetol.
14, 145 (2015),
Application(s): Immunoblot analysis,
Abstract;
Full Text
Proprotein convertases play an important role in regulating PKGI endoproteolytic cleavage and nuclear transport: S. Kato, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol.
305, L130 (2013),
Application(s): Western blot,
Abstract;
Full Text
Protein kinase G oxidation is a major cause of injury during sepsis: O. Rudyk, et al.; PNAS
110, 9909 (2013),
Application(s): Western blot,
Abstract;
Full Text
Hydrogen peroxide enhances vasodilatation by increasing dimerization of cGMP-dependent protein kinase type Iα: D. Dou, et al.; Circ. J.
76, 1792 (2012),
Application(s): Western blot,
Abstract;
Nitroglycerin fails to lower blood pressure in redox-dead Cys42Ser PKG1α knock-in mouse: O. Rudyk, et al.; Circulation
126, 287 (2012),
Application(s): Western blot,
Abstract;
Full Text
cGMP-dependent protein kinase I beta physically and functionally interacts with the transcriptional regulator TFII-I: R.B. Pilz, et al. ; J. Biol. Chem.
277, 32003 (2002),
Application(s): IP (M), ICC (H) using mouse samples,
Abstract;
Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation: S.A. Fisher, et al. ; J. Biol. Chem.
276, 37250 (2001),
Application(s): IP, WB using rat samples,
Abstract;
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