Product Details
Alternative Name: | Phosphoinositide-3-kinase |
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Clone: | AB6 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant human PI3 kinase (p85α). |
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UniProt ID: | P27986 |
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Species reactivity: | Human, Mouse, Rat
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Specificity: | Recognizes the p85α subunit of PI3 kinase. |
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Applications: | ICC, IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (10µg/ml) Immunoprecipitation (5-10µg/ml per 100µg cell extract) Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~85kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Phosphatidylinositol 3-kinase (PI3-K) is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3 |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of PI3 kinase expression in Jurkat (1), Raji (2), U937 (3), WR19L (4) and PC12 (5) using PI3-kinase, p85a mAb (AB6).
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Product Literature References
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
15, 909 (2018),
Abstract;
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