| ADI-KAM-CC210-E | 100 µg | 414.00 USD |  |
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| Clone: | 1C5 |
| Host: | Mouse |
| Isotype: | IgG2a |
| Immunogen: | Recombinant human DNA-topoisomerase IIα (C-terminal fragment). |
| UniProt ID: | P11388 |
| Species reactivity: | Human |
| Applications: | Flow Cytometry, ICC, IHC, IP, WB |
| Recommended Dilutions/Conditions: | Flow Cytometry (0.1-1.0µg/ml) Immunocytochemistry (1-10µg/ml) Immunohistochemistry (1-5µg/ml) Immunoprecipitation (1-10µg/ml, 200-300ml cell extract) Western Blot (0.1-0.5µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of 170kDa by Western blot. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | DNA-topoisomerase II, also known as Topo II or Top 2, is a multifunctional nuclear enzyme required during DNA replication, chromosome disjunction at mitosis, and other DNA-related activities because of its ability to alter DNA supercoiling. In mammalian cells, Topo II consists of two isozymes, Topo II-alpha (170kDa) and Topo II-beta (180kDa) and expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo II-beta is expressed constitutively throughout the cell cycle, whereas the expression of Topo II-alpha is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. DNA-topoisomerase II is the target of some of the most active drugs used in chemotherapy and has been shown to have a critical role in neural development. |
| Regulatory Status: | RUO - Research Use Only |
Goat anti-mouse IgG F(ab')2, polyclonal antibody (HRP conjugate) |
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| ELISA, IHC, WB | Print as PDF | ||||||
| ADI-SAB-100-J | 1 ml | 154.00 USD | |
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Jurkat, (cell lysate) |
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| Human, WB | Print as PDF | ||||||
| ADI-LYC-JK100-F | 200 µg | 143.00 USD | |
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