Product Details
| Alternative Name: | TRP53, Tumor protein p53 |
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| Clone: | pAb122 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | SV40 transformed 3T3 cells. |
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| UniProt ID: | P02340 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Human, Mouse, Rat
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| Crossreactivity: | Weakly cross-reacts with dog p53. |
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| Applications: | Flow Cytometry, ICC, IHC, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:250 colorimetric, 1:250 ECL)
Immunohistochemistry (1:20 - 1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~48/53kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to Mdm2, SV40 T antigen and human Papilloma virus E6 protein. p53 senses DNA damage and facilitates its repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovary, bladder, colon, lung, and melanoma. |
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| Regulatory Status: | RUO - Research Use Only |
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Endometrial carcinoma stained by immunohistochemistry with p53 monoclonal antibody (PAb122) (ENZ-ABS849, 1:20 Dilution).
Western blot analysis of p53: Lane 1: MW marker, Lane 2: A431, Lane 3: rat brain, Lane 4: mouse brain.
Endometrial carcinoma stained by immunohistochemistry with p53 monoclonal antibody (PAb122) (
ENZ-ABS849, 1:20 Dilution).
Flow cytometry analysis of 10^6 Jurkat cells stained using p53 monoclonal antibody (PAb122), (Prod. No. ENZ-ABS849), at a concentration of 50 µg/mL.
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Product Literature References
Pulmonary tumors associated with the JC virus T-antigen in a transgenic mouse model: A. Noguchi, et al.; Oncol. Rep.
30, 2603 (2013),
Application(s): Immunohistochemistry of mouse tissue,
Abstract;
Full Text
Abdominal temperature induces region-specific p53-independent apoptosis in the cauda epididymidis of the mouse: M. Jara, et al.; Biol. Reprod.
67, 1189 (2002),
Application(s): WB using mouse tissue homogenates,
Abstract;
Full Text
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