Caspase-3 polyclonal antibody



ADI-AAS-103-D	50 µl	199.00 USD

ADI-AAS-103-F	200 µl	415.00 USD
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Product Details

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to a portion of human caspase-3 . The sequence differs from rat caspase-3 by 1 aa and from mouse by 2 aa.

UniProt ID:	P42574

GenBank ID:	U26943

Source:	Whole rabbit serum.

Species reactivity:	Human, Mouse, Rat Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus

Applications:	WB

Recommended Dilutions/Conditions:	Western Blot (ECL, 1:5,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~36kDa by Western blot.

Formulation:	Liquid. Antiserum.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or ?executioner? caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.

Regulatory Status:	RUO - Research Use Only

Caspase-3 polyclonal antibody Western blot

Western Blot Analysis of Caspase-3, pAb (Prod. No. ADI-AAS-103): Lane 1:MW Marker; Lane 2: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100); Lane 3: Jurkat Cell Lysate (Prod. No. ADI-LYC-JK100); Lane 4: Jurkat + Staurosporine

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Product Literature References

Novel gold(I) complexes induce apoptosis in leukemia cells via the ROS-induced mitochondrial pathway with an upregulation of Harakiri and overcome multi drug resistances in leukemia and lymphoma cells and sensitize drug resistant tumor cells to apoptosis in vitro: M.-C. Ahrweiler-Sawaryn, et al.; Biomed. Pharmacother. 161, 114507 (2023), Abstract;

Lactucin induces apoptosis through reactive oxygen species-mediated BCL-2 and CFLARL downregulation in Caki-1 cells: J.H. Jang, et al.; Genes Genomics 43, 1199 (2021), Abstract;

Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species: J. Cabrera, et al.; Chem. Biol. Interact. 256, 220 (2016), Application(s): Western Blot, Abstract;

Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Western Blot, Abstract;

Protective effect of geranylgeranylacetone against radiation-induced delayed effects on human keratinocytes: M. Isoir, et al.; Radiat. Res. 179, 232 (2013), Abstract;

Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis: R.E. Lee, et al. ; Apoptosis 12, 1183 (2007), Application(s): WB using drosophila cell lysates, Abstract;

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