| ADI-AAP-221-D | 50 µg | 256.00 USD |  |
||
| ADI-AAP-221-F | 200 µg | 486.00 USD | |
||
| Do you need bulk/larger quantities? | |||||
| Alternative Name: | CD95, APO-1, TNFRSF 6 |
| Host: | Rabbit |
| Immunogen: | Synthetic peptide corresponding to a portion of mouse Fas. |
| UniProt ID: | P25446 |
| GenBank ID: | M83649 |
| Source: | Purified from rabbit serum. |
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC, WB |
| Recommended Dilutions/Conditions: | Immunohistochemistry (1:50) Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~45kDa by Western blot. |
| Purity Detail: | Peptide affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | Fas/APO-1/CD95 is a 36 kDa cell surface type I-membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas belongs to the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells. Both Fas and TNFR contain a 70 amino acid cytoplasmic “death domain” responsible for transmitting signals for apoptosis. Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long forms of FLIP. Insertion of an early transposable element in intron 2 of the Fas gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting the involvement of Fas in clonal deletion of autoreactive T cells. |
| Regulatory Status: | RUO - Research Use Only |
Goat anti-rabbit IgG, polyclonal antibody (HRP conjugate) |
||||||
| ELISA, IHC, WB | Print as PDF | ||||||
| ADI-SAB-300-J | 1 ml | 154.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HeLa, (cell lysate) |
||||||
| Human, WB | Print as PDF | ||||||
| ADI-LYC-HL100-F | 200 µg | 141.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Brain (rat), (tissue extract) |
||||||
| Rat brain extract, WB | Print as PDF | ||||||
| ADI-LYT-RB100-F | 200 µg | 225.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
