- Cost effective - assay ~200 samples in duplicate for the cost of one ready-to-use ELISA
- Accurate - fully quantitative results surpass semi-quantitative Western blot analysis
- Reliable - thoroughly validated in complex sample matrices
The IMMUNOSET® PDI ELISA development set contains sufficient reagents for the development of 5 x 96 well ELISA plates which provides analysis of ~200 samples in duplicate. Save time, money, and sample by developing your own ELISA kits. Simply coat your plates and go! This kit provides superior accuracy with fully quantitative results compared to Western blot analysis and has low reactivity with related molecules.
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Product Details
| Alternative Name: | Protein disulfide isomerase, P4HB |
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| Sensitivity: | 3.93 ng/ml (range 7.8 - 250 ng/ml) |
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| Assay Time: | Plate Coating - Overnight + 1 hour; Assay - 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of PDI in cell lysates, culture supernatants, plasma, and tissue of human, mouse, and rat origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human, Mouse, Rat
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| Crossreactivity: | PDI (100%), Grp94 (0.121%), Hsc70 (0.018%) and 0.016%: Calreticulin, Grp78, HSP90α, HSP90β, and HSP70 |
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| Contents: | Capture antibody, Standard, Detection antibody, SA-HRP |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | The mammalian PDI (Protein disulfide-isomerase) family encompasses several highly divergent proteins which are involved in the processing and maturation of secretory proteins in the endoplasmic reticulum by catalyzing the rearrangement of disulfide bonds. |
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| UniProt ID: | P07237 (human) |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Protein disulfide isomerase modulation of TRPV1 controls heat hyperalgesia in chronic pain: Y. Zhang, et al.; Cell Rep.
39, 110625 (2022),
Abstract;
Expression and release of platelet protein disulphide isomerase in patients with haemophilia A: M. Voigtlaender, et al.; Haemophilia
22, e537 (2016),
Abstract;
