IHC enzyme antigen retrieval reagent is designed to unmask immunoreactive sites altered by fixation and/or the embedding process.
Product Details
| Formulation: | Liquid. Enzyme antigen retrieval reagent. |
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| Appearance: | Clear slightly viscous solution. |
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| Applications: | IHC
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | The most commonly used tissue fixatives are aldehydes, such as formalin, which work by protein cross-linking. Proper fixation of a specimen prevents antigen from being washed out and is a critical aspect of successful IHC staining. Cross-linking can mask antigens, preventing primary antibodies from binding, thus yielding weak staining or false negative results. Immunoglobulins are especially susceptible to formalin fixation. Treatment with a proteolytic enzyme is required to digest excess aldehyde linkages and expose the antigen. The IHC enzyme antigen retrieval reagent is a proteolytic enzyme solution that results in superior staining over traditionally used enzyme pretreatments, such as pepsin and trypsin. Moreover, the IHC enzyme antigen retrieval reagent requires only a brief incubation at room temperature. Performing antigen recovery with the IHC enzyme antigen retrieval reagent will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining. |
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| Technical Info/Product Notes: | Interpretation of the results will be the sole responsibility of the user. |
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| Protocol: | Digestion Procedure
1. Deparaffinize tissue sections and bring them to buffer.
2. Remove excess buffer from slides without letting tissue dry.
3. Depending on tissue section size, add 1 or 2 drops of IHC enzyme antigen retrieval reagent.
4. Incubate slides for 5 minutes at room temperature.
5. Wash slides with buffer and proceed with immunostaining. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Spinal Cord Sensitization and Spinal Inflammation from an In Vivo Rat Endplate Injury Associated with Painful Intervertebral Disc Degeneration: A. Lai, et al.; Int. J. Mol. Sci.
24, 3425 (2023),
Abstract;
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