The IHC tissue primer is a universal enzyme blocking reagent to use in horse radish peroxidase (HRP) and alkaline phosphatase (AP) based immunohistochemical staining procedures done on paraffin tissue sections, frozen tissue sections and cell smears to suppress nonspecific staining due to endogenous enzyme activity.
Product Details
| Formulation: | Liquid. Tissue primer. TRIS-based alkaline solution with 30% H2O2. |
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| Applications: | IHC
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Handling: | Store tightly sealed in the dark. Protect from light. |
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| Scientific Background: | In the evaluation of tissue specimens by IHC using HRP and AP methods, the presence of endogenous enzymes can often obscure the specific staining of the target antigen. Endogenous peroxidase and AP activity can be inhibited by the use of the IHC tissue primer which will suppress endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in paraffin tissue sections, frozen tissue sections and cell smears. |
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| Technical Info/Product Notes: | Interpretation of the results will be the sole responsibility of the user. |
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| Protocol: | For paraffin-embedded tissues, deparaffinize using standard histological methods. Perform any required pre-treatment steps (such as antigen retrieval) prior to using the IHC tissue primer. Incubate deparaffinized tissue sections, frozen tissue sections or cell smears for 5 to 10 minutes at room temperature prior to applying primary antibody. Rinse tissue with wash buffer after incubation with IHC tissue primer and proceed to the next step. |
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| Regulatory Status: | RUO - Research Use Only |
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