IHC universal negative control reagent is specifically designed to work with rabbit, mouse and goat antibodies. The reagent has been titered to work with polymer-based secondary systems in manual and automated IHC platforms.
Product Details
| Formulation: | Liquid. Negative control reagent- mixture of purified goat, mouse and rabbit immunoglobulins (IgGs). |
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| Applications: | IHC
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Negative controls are used to determine the specificity of an antibody. No staining should be observed when the primary antibody is omitted. Minimal non-specific staining should be detected when using the IHC universal negative control reagent. |
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| Technical Info/Product Notes: | Interpretation of the results will be the sole responsibility of the user. Source is from purified goat, mouse and rabbit immunoglobulins (IgGs) and total IgG concentration is 1.5 ug/ml. |
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| Protocol: | Use IHC universal negative control reagent in place of the primary antibody with a section to evaluate non-specific staining and allow better interpretation of staining results. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Extracellular matrix proteins (fibronectin, collagen III, and collagen I) immunoexpression in goat tuberculous granulomas (Mycobacterium caprae): C. Neila, et al.; Vet. Res. Commun.
46, 1147 (2022),
Abstract;
Natural infection of parvovirus in wild fishing cats (Prionailurus viverrinus) reveals extant viral localization in kidneys: C. Piewbang, et al.; PLoS One
16, 247266 (2021),
Abstract;
TRPV Subfamily (TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6) Gene and Protein Expression in Patients With Ulcerative Colitis: J.J.T. Maurino, et al.; J. Immunol. Res.
2020, 2906845 (2020),
Abstract;
Full Text
Inflammatory chemokine profiles and their correlations with effector CD4 T cell and regulatory cell subpopulations in cutaneous lupus erythematosus: S. Mendez-Flores, et al.; Cytokine
119, 95 (2019),
Abstract;
Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease: J.K. Yamamoto-Furusho, et al.; J. Inflamm. (Lond.)
15, 13 (2018),
Abstract;
Full Text
Gelatinase B/Matrix Metalloproteinase-9 as Innate Immune Effector Molecule in Achalasia: J. Furuzawa-Carballeda, et al.; Clin. Transl. Gastroenterol.
9, 208 (2018),
Application(s): Tissue biopsies from achalasia patients,
Abstract;
Full Text
