The HIGHDEF® IHC enhancer (DAB, plus) is a DAB signal enhancer that can be used to increase intensity of immunostaining utilizing DAB as chromogen.
Product Details
| Formulation: | Liquid. 1X DAB chromogen enhancer. |
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| Appearance: | Amber solution |
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| Applications: | IHC
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | Ambient |
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| Technical Info/Product Notes: | One of the hallmarks of successful IHC is the strength of the signal generated during immunostaining. DAB, widely used as a chromogen for immunoperoxidase staining, has been well accepted by pathologists due to its superior performance compared to amino ethyl carbazol (AEC). Tissue specimens stained with DAB after only a brief incubation show increased staining intensity when the enhancer is applied. Increased sensitivity of the detection system allows the cost-effective option of diluting the primary antibody 5 to 10 fold. HIGHDEF® IHC enhancer (DAB, plus) increases the signal intensity without creating any background staining. |
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| Protocol: | - Follow standard IHC protocol.
- After incubating sections with DAB reagent, rinse slides with distilled water.
- Apply HIGHDEF® IHC enhancer (DAB, plus) solution for 60 seconds.
- Rinse in buffer and continue with counterstaining.
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Epizootics due to Yellow Fever Virus in São Paulo State, Brazil: viral dissemination to new areas (2016–2017): M.S. Cunha, et al.; Sci. Rep.
9, 5474 (2019),
Abstract;
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