- Universal - suitable for any AcetylCoA-dependent acetyltransferase
- Robust - Z' factor = 0.87
- Sensitive - fluorescent detection allows for both DNA and protein assays
- Higher throughput - homogeneous mix-and-read assay
The Acetyltransferase activity kit is a homogeneous mix-and-read fluorescent assay for the determination of any acetyl-CoA dependent acetyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.
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Product Details
| Applications: | Fluorescent detection, HTS
Activity assay
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| Application Notes: | For the measurement of acetyl-CoA dependent acetyltransferase activity in purified in vitro samples that do not contain reductants from any species. |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at -20°, except positive control at -80°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Black Microtiter plate, Reaction buffer, Assay buffer, Detection solution, Positive control |
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| Scientific Background: | Acetylation of proteins and drugs is a regulatory event that can result in the activation, inactivation, or detoxification of molecules including histones, kinases, transcription factors, anti-microbial agents, and neurotransmitters. While acetylation can be carried out by a variety of acetyltransferase enzymes using diverse substrates, their common cofactor or acetyl donor is acetyl-CoA. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Structure of Thermoplasma volcanium Ard1 belongs to N-acetyltransferase family member suggesting multiple ligand binding modes with acetyl coenzyme A and coenzyme A: C. Ma, et al.; Biochim. Biophys. Acta
1844, 1790 (2014),
Application(s): Acetyltransferase purified from bacteria,
Abstract;
