Product Details
| Alternative Name: | EDNRB, ETB |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of rat endothelin receptor ETB. |
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| UniProt ID: | P21451 |
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| Species reactivity: | Rat
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| Crossreactivity: | Predicted species reactivity based on epitope sequence homology: human, mouse, prairie deer mouse, beach mouse, rhesus macaque, chimpanzee, horse, dog. |
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| Applications: | IHC, WB
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| Application Notes: | Predicted MW of ~50kDa. Higher MW species (~ 55kDa) and intermediates also observed by Western blot which reflect post-translational modification of the receptor. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. |
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| Handling: | Keep on ice at all times. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Endothelins are vasoactive peptides that exist in three forms (ET-1, ET-2, and ET-3), and function in the maintenance of vascular tone via two identified seven transmembrane-spanning domain-containing G protein-coupled receptors (GPCR), endothelin A (ETA) and endothelin B (ETB). The human ETA receptor is primarily expressed in vascular smooth muscle cells, including those of the heart (also in myocytes), lung, and brain, and its stimulation generally results in vasoconstriction. Activation of ETA receptors can be stimulated by binding of ET-1 and ET-2, but not ET-3. triggering the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells. Human ETB receptors are primarily expressed in endothelial cells lining the vessel walls of the lungs, heart, and brain, and in contrast to ETA receptors, stimulation generally results in transient vasodilation. Activation of ETB receptors can be stimulated by binding of all three endothelins, triggering the release of vasorelaxive |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Manufactured by Proteimax. |
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Western blot analysis: MW marker (1) and 20µg rat heart membrane extract (2) probed with Endothelin Receptor (ETB) pAb at 3.4µg/ml.
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Product Literature References
Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One
12, e0187306 (2017),
Abstract;
Full Text
Fibroblast growth factor and endothelin-1 receptors mediate the response of human striatal precursor cells to hypoxia: S. Ambrosini, et al.; Neuroscience
289C, 123 (2015),
Application(s): Western Blotting,
Abstract;
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