Alternative size available: ADI-900-023 (1x96 wells)
High sensitivity, measuring as low as 36.8 pg/ml
Fast results, much less time consuming than GC/MS analysis
Simple and ready-to-use liquid color-coded reagents reduce error
Fully quantitative results that surpass semi-quantitative Western blot analysis
Save with bulk kit package!
The 15-deoxy-Δ12,14-PGJ2 EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. This kit saves time and is much less time consuming than GC/MS analysis. Ready-to-use liquid color-coded reagents reduce laboratory error.
Product Details
Alternative Name:
15-deoxy-Δ12,14-Prostaglandin J2
Sensitivity:
36.8 pg/ml (range 195 - 200,000 pg/ml)
Assay Time:
5 hours
Applications:
ELISA, Colorimetric detection
Application Notes:
For the quantitative determination of 15-deoxy-Δ12,14-PGJ2 in culture supernatants, plasma, saliva, and urine from any species.
Wavelength:
405 nm
Species reactivity:
Species independent
Use/Stability:
Store all components at 4°C, except standard and conjugate at -20°C.
15-deoxy-Δ12,14-Prostaglandin J2 (15-d-PGJ2) is one of the ultimate dehydration products of PGD2, formed via the intermediate Δ12-PGJ2. 15-d-PGJ2 is physiologically present in body fluids at concentration of 10-12 to 10-9 M, but increases dramatically under stress such as infection and inflammation. 15-d-PGJ2 exerts both pro- and anti-inflammatory effects in a cell-type and concentration-dependent manner, with many of these effects mediated via binding to the γ isoform of the peroxisome proliferators-activated receptor (PPAR-γ). 15-d-PGJ2 appears to induce HO-1 expression by a PPAR-γ-independent mechanism mediated by the generation of reactive oxygen species (ROS).
Redox aspects of cytotoxicity and anti-neuroinflammatory profile of chloroquine and hydroxychloroquine in serum-starved BV-2 microglia: L. Račková, et al.; Toxicol. Appl. Pharmacol. 447, 116084 (2022), Abstract;
Fluoxetine‐induced hepatic lipid accumulation is mediated by prostaglandin endoperoxide synthase 1 and is linked to elevated 15‐deoxy‐Δ12, 14PGJ3: A. Ayyash, et al.; J. Appl. Toxicol. 4272, 1002 (2021), Abstract;
Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis: C.D. Wiley, et al.; Cell Metab. 33, 1124 (2021), Abstract;
Cocaine-mediated circadian reprogramming in the striatum through dopamine D2R and PPARγ activation: K. Brami-Cherrier, et al.; Nat. Commun. 11, 4448 (2020), Abstract; Full Text
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway: Y. Zhang, et al.; FASEB J. 34, 11772 (2020), Abstract; Full Text
Apolipoprotein D overexpression alters hepatic prostaglandin and omega fatty acid metabolism during the development of a non-inflammatory hepatic steatosis: F. Desmarais, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864, 522 (2019), Application(s): ELISA using liver homogenates, Abstract;
Association of chronic inflammation and perceived stress with abnormal functional connectivity in brain areas involved with interoception in hepatitis C patients: G. Oriolo, et al.; Brain Behav. Immun. 80, 204 (2019), Application(s): ELISA using human serum, Abstract;
Programming of macrophages by UV-irradiated apoptotic cancer cells inhibits cancer progression and lung metastasis: Y.B. Kim, et al.; Cell. Mol. Immunol. 16, 851 (2019), Application(s): ELISA using culture supernatants, Abstract;
Arachidonic acid induces ARE/Nrf2-dependent heme oxygenase-1 transcription in rat brain astrocytes: C.C. Lin, et al.; Mol. Neurobiol. 55, 3328 (2018), Abstract;
Bone-marrow-derived mesenchymal stem cells inhibit gastric aspiration lung injury and inflammation in rats: J. Zhou, et al.; J. Cell Mol. Med. 20, 1706 (2016), Application(s): 15d-PGJ2 was measured from blood samples, Abstract; Full Text
Implications of a genetically determined nitric oxide deficit for endothelial cell-leukocyte interaction and cardiovascular disease: I. Kadiyska; (2016), (PhD thesis); Application(s): Quantitative determination of 15d-PGJ2 in culture supernatants, Full Text
CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis: M. Ishii, et al.; J. Immunol. 188, 5655 (2012), Abstract;
Choroidal involution is a key component of oxygen-induced retinopathy: Z. Shao, et al.; Invest. Ophthalmol. Vis. Sci. 52, 6238 (2011), Application(s): ELISA using rat tissue lysate, Abstract; Full Text
Response of chondrocytes to shear stress: antagonistic effects of the binding partners Toll-like receptor 4 and caveolin-1: P. Wang, et al.; FASEB J. 25, 3401 (2011), Application(s): ELISA using human cell culture medium, Abstract; Full Text
The myocardial infarct size-limiting effect of sitagliptin is PKA-dependent, whereas the protective effect of pioglitazone is partially dependent on PKA: Y. Ye, et al.; Am. J. Physiol. Heart Circ. Physiol. 298, H1454 (2010), Application(s): ELISA using mouse tissue lysate, Abstract; Full Text
Celecoxib Antagonizes Perifosine's Anticancer Activity Involving a Cyclooxygenase-2-Dependent Mechanism: H. A. Elrod, et al.; Mol. Cancer Ther. 8, 2575 (2009), Application(s): ELISA using human cell culture medium, Abstract; Full Text
A novel natural compound, a cycloanthranilylproline derivative (Fuligocandin B), sensitizes leukemia cells to apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) through 15-deoxy-Delta 12, 14 prostaglandin J2 production: H. Hasegawa, et al.; Blood 110, 1664 (2007), Application(s): ELISA using human cell lysates, Abstract; Full Text
Interleukin-13 enhances cyclooxygenase-2 expression in activated rat brain microglia: implications for death of activated microglia: M. S. Yang, et al.; J. Immunol. 177, 1323 (2006), Application(s): ELISA using rat cell culture medium, Abstract; Full Text
The selective cyclooxygenase-2 inhibitor SC-236 reduces liver fibrosis by mechanisms involving non-parenchymal cell apoptosis and PPARgamma activation: A. Planagumà, et al.; FASEB J. 19, 1120 (2005), Application(s): ELISA using rat liver cell lysates, Abstract; Full Text
A novel antipyretic action of 15-deoxy-Delta12,14-prostaglandin J2 in the rat brain: A. Mouihate, et al.; J. Neurosci. 24, 1312 (2004), Application(s): ELISA using rat cerebrospinal fluid, Abstract; Full Text
Proliferative action of mast-cell tryptase is mediated by PAR2, COX2, prostaglandins, and PPARgamma: Possible relevance to human fibrotic disorders: M.B. Frungieri, et al.; PNAS 99, 15072 (2002), Application(s): EIA using human cell lysates, Abstract;
Transgenic cyclooxygenase-2 overexpression sensitizes mouse skin for carcinogenesis: K. Muller-Decker, et al.; PNAS 99, 12483 (2002), Application(s): ELISA using mouse tissue lysate, Abstract; Full Text
