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Rapid - fully quantitative results in < 3 hours
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Convenient – user-friendly alternative to mass spectrometry
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Simple – efficient and well-established protocol
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High-throughput – 96 well format ideal for global detection, tissue-specific quantitation, high-throughput compound screening, and more
The 5-Hydroxymethylcytosine DNA ELISA kit is a complete, colorimetric, immunometric immunoassay kit for the quantitative determination of 5-hydroxymethylcytosine in DNA with results in just 3 hours.
DNA Mass Spectroscopy versus ELISA analysis: The 5-hmC DNA ELISA Kit (prod. no. ADI-900-225) quantifies 5-hmC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. DNA samples include: brain, kidney, liver, thymus, human embryonic stem cells and two carcinoma samples.
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Product Details
| Alternative Name: | 5-hmC |
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| Sensitivity: | <0.02% 5-hydroxymethylcytosine DNA per 100ng input DNA (range 0.03-0.55% (100 ng/µl)) |
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| Assay Time: | ~3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of species independent 5-hydroxymethylcytosine DNA in a variety of DNA samples. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at 4°C, except antibody and control set should be stored at -20°C, while the DNA HRP antibody should be stored at -80°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Microtiter Plate, Coating Buffer, ELISA Buffer, Primary Antibody, Antibody-conjugate, HRP Developer, Controls |
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| Scientific Background: | The 5-methylcytosine (5-mC) modification of DNA in epigenetic regulation has been well studied over the last several decades. However, the role of the so-called “sixth base”, 5-hydroxymethylcytosine (5-hmC), has yet to be defined. Recent studies have associated 5-hmC patterns to transcriptional regulation of genes, which may contribute to normal and disease states of organisms. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Atm deficiency in the DNA polymerase β null cerebellum results in cerebellar ataxia and Itpr1 reduction associated with alteration of cytosine methylation: J. Kim, et al.; Nucleic Acids Res.
48, 3678 (2020),
Abstract;
Full Text
General Literature References
Alterations of the lung methylome in allergic airway hyper-responsiveness: R.Y. Cheng, et al.; Environ. Mol. Mutagen.
55, 244 (2014),
Abstract;
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