- Measure low levels of rat Big ET-1
- Convenient 4°C storage
- Assay up to 38 samples in duplicate per plate
The Big Endothelin-1 (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results overnight + 1 hour.
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Product Details
| Alternative Name: | Big ET-1 |
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| Sensitivity: | 0.72 pg/ml (range 0.78 - 100 pg/ml) |
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| Assay Time: | Overnight + 1 hour |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of rat Big ET-1 in culture supernatants, plasma, and serum. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Rat
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter plate, Labeled antibody concentrate, Antibody diluent, Assay buffer, Wash buffer concentrate, Standard, TMB Substrate, Stop solution |
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| Scientific Background: | Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure. |
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| UniProt ID: | P22388 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Fasudil preserves lung endothelial function and reduces pulmonary vascular remodeling in a rat model of end‑stage pulmonary hypertension with left heart disease: R. Zhuang, et al.; Int. J. Mol. Med.
42, 1341 (2018),
Application(s): ELISA using rat serum,
Abstract;
Full Text
Consequences of a Maternal High-Fat Diet and Late Gestation Diabetes on the Developing Rat Lung: M.L. Baack, et al.; PLoS One
11, e0160818 (2016),
Application(s): Milliplex assays performed on lung lysate,
Abstract;
Full Text
Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity: A.R. Ko, et al.; Mol. Brain
8, 58 (2015),
Application(s): ELISA measuring ET-1 in rats ,
Abstract;
Full Text
Value of Caffeic Acid Phenethyl Ester Pretreatment in Experimental Sepsis Model in Rats: O. Alici, et al.; Mediators Inflamm.
2015, 810948 (2015),
Application(s): ELISA using rat serum,
Abstract;
Full Text
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