- Broad dynamic range suitable for many sample types
- Stable liquid color-coded reagents reduce errors
- Convenient storage conditions
- Results from up to 36 samples in duplicate in less than 3 hours
- Reproducible results day-after-day and lot-after-lot
The PGF2α EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Broad dynamic range is suitable for a wide variety of sample types. Ready-to-use liquid color-coded reagents are safe, non-radioactive, and reduce laboratory errors.
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Product Details
| Alternative Name: | Prostaglandin F2α |
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| Sensitivity: | 6.71 pg/ml (range 3.05 - 50,000 pg/ml) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of PGF2α in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample types include cell lysate and whole blood. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with preeclampsia. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Gestational NSAIDs distinctly reprogram cardiac injury in preeclamptic rats: Roles of cyclooxygenase, apoptotic and autophagic trails: M.A. Ali, et al.; Life Sci.
310, 121130 (2022),
Abstract;
Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol.
12, 774086 (2022),
Application(s): ELISA kit using hemolymph,
Abstract;
Full Text
Piezo1 activation induces fibronectin reduction and PGF2α secretion via arachidonic acid cascade: W. Morozumi, et al.; Exp. Eye Res.
215, 108917 (2022),
Abstract;
Effect of proinflammatory cytokines on endometrial collagen and metallopeptidase expression during the course of equine endometrosis: A.Z. Szóstek-Mioduchowska, et al.; Cytokine
123, 154767 (2019),
Application(s): Horse endometrial tissue,
Abstract;
The role of embryo contact and focal adhesions during maternal recognition of pregnancy: K.M. Klohonatz, et al.; PLoS One
14, e0213322 (2019),
Abstract;
Full Text
Elastase inhibition affects collagen transcription and prostaglandin secretion in mare endometrium during the estrous cycle: A. Amaral, et al.; Reprod. Domest. Anim.
53, 66 (2018),
Abstract;
Oxidative Stress is a Convincing Contributor to Idiopathic Chronic Fatigue: J.S. Lee, et al.; Sci. Rep.
8, 12890 (2018),
Abstract;
Full Text
The influence of experimentally induced endometritis on the PPAR expression profile in the bovine endometrium: B.M. Socha, et al.; Theriogenology
122, 74 (2018),
Application(s): ELISA using culture supernatants,
Abstract;
Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol.
89, 19 (2017),
Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates,
Abstract;
Electrochemical immunosensor for the determination of 8-isoprostane aging biomarker using carbon nanohorns-modified disposable electrodes: E.E Sánchez-Tirado, et al.; J. Electroanal. Chem. 793, 197 (2017),
Circulating retinoic acid levels and the development of metabolic syndrome: Y. Liu, et al.; J. Clin. Endocrinol. Metab.
101, 1686 (2016),
Application(s): Measured plasma 8-iso-Prostaglandin F2,
Abstract;
Full Text
Regulation of cyclooxygenase-2 expression in rat oviductal epithelial cells: Evidence for involvement of GPR30/Src kinase-mediated EGFR signaling: P. Popli, et al.; J. Steroid Biochem. Mol. Biol.
154, 130 (2015),
Application(s): ELISA using rat cell lysate,
Abstract;
Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol.
180, 56 (2013),
Application(s): ELISA using aquarium water,
Abstract;
Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol.
165, 277 (2010),
Application(s): EIA using fish plasma and water samples,
Abstract;
Discovery of ecopladib, an indole inhibitor of cytosolic phospholipase A2 alpha: K.L. Lee, et al. ; J. Med. Chem.
50, 1380 (2007),
Application(s): EIA using human whole blood,
Abstract;
Cloning, Expression, and Functional Characterization of Human Cyclooxygenase-1 Splicing Variants: Evidence for Intron 1 Retention: N. Qin, et al. ; J. Pharmacol. Exp. Ther.
315, 1298 (2005), Application(s): EIA using human cell lysates,
Abstract;
17{beta}-Estradiol decreases vascular tone in cerebral arteries by shifting COX-dependent vasoconstriction to vasodilation: J.A. Ospina, et al. ; Am. J. Physiol. Heart Circ. Physiol.
285, H241 (2003),
Application(s): EIA using rat cell lysates,
Abstract;
Skeletal Muscle PGF2{alpha}and PGE2 in Response to Eccentric Resistance Exercise: Influence of Ibuprofen and Acetaminophen: T.A. Trappe, et al. ; J. Clin. Endocrinol. Metab.
86, 5067 (2001),
Application(s): EIA using human cell lysates,
Abstract;
Tumor Necrosis Factor-alpha Induces Cyclooxygenase-2 Expression and Prostaglandin Release in Brain Microvessel Endothelial Cells: K.S. Mark, et al. ; J. Pharmacol. Exp. Ther.
297, 1051 (2001),
Application(s): EIA using bovine cell lysates,
Abstract;
Phase-dependent influence of nonsteroidogenic cells on steroidogenesis and prostaglandin production by the human corpus luteum: B.E. Friden, et al. ; Fertil. Steril.
73, 359 (2000),
Application(s): EIA using human culture supernatants,
Abstract;
Prostaglandin-Independent Anovulatory Mechanism of Indomethacin Action: Inhibition of Tumor Necrosis Factor {alpha}-Induced Sheep Ovarian Cell Apoptosis: W.J. Murdoch, et al. ; Biol. Reprod.
61, 1655 (1999),
Application(s): EIA using sheep cell lysates,
Abstract;
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