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Redder MMP Substrates. Better Activity Assays.

Extensive MMP Product Range

Proteins of the matrix metalloproproteinase (MMP) family are collectively involved in the breakdown of extracellular matrix proteins and processing of bioactive molecules and are required for some normal processes. Unfortunately, these proteins are also associated with many pathological processes including cirrhosis, arthritis, metastasis, and chronic cardiovascular diseases. Collagenases (Matrix Metalloproteinase-1, MMP-8, and MMP-13) and gelatinases (MMP-2 and MMP-9) also break down skin collagens, contributing to aging and wrinkling. Enzo offers high sensitivity colorimetric and fluorometric activity assays for screening collagenase and gelatinase inhibitors as well as highly active enzymes and specific antibodies for all your MMP research needs.

 
Product Types
Assay Kits Substrates Active Proteins Antibodies Inhibitors
Broad Spectrum MMP Analysis Find Find Find Small Molcules / TIMPs
MMP-1 Find Find Find Find
MMP-2 Find Find Find Find Find
MMP-3 Find Find Find Find Find
MMP-7 Find Find Find Find
MMP-8 Find Find Find
MMP-9 Find Find Find Find
MMP-10 Find Find Find Find
MMP-11 Find Find
MMP-12 Find Find Find Find
MMP-13 Find Find Find Find
MMP-14 Find Find Find
MMP-19 Find Find Find
MMP-20 Find Find Find
MMP-24 Find Find

Ultra-sensitive, long wavelength fluorogenic substrates reduce interference.

The MMP RED and GREEN Drug Discovery Kits are complete assay systems designed to screen MMP inhibitors, using long-wavelength quenched fluorogenic substrates. The assays are performed in a convenient 96-well microplate format, with the compound NNGH [1] also included as a prototypic control inhibitor.

  • Improved red-shifted substrate with better kinetics and brighter signal
  • Includes active recombinant enzyme, substrate, and assay buffer
  • Convenient real-time kinetics of cleavage is easily determined
  • Microplate format for high-throughput screening
Figure 1: Fluorescent emission spectra of small molecule drug camptothecin (light blue), OE33 cells (dark blue), MMP GREEN substrate (green), and MMP RED substrate (red), showing that the MMP GREEN and RED substrates avoid the interference caused by small molecules and cells.
 

OMNIMMP™ RED and MMP-3 Fluorogenic substrates offer key advantages over other MMP substrates

  • Emission at the higher end of the spectrum (576nm and 521nm, respectively, after excitation at 545nm and 494nm) avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium.
  • Highly quenched (very low background), but once cleaved by MMPs emits extremely bright signal.
  • MMP substrate peptides inherently display poor aqueous solubility, often with KmS near their limits of solubility, making enzyme and inhibitor kinetics difficult. MMP KmS for OMNIMMP™ RED and MMP-3 Fluorogenic Substrates are well below the solubility limits of the substrates.
  • In addition to the efficient binding as exhibited by low KmS, OMNIMMP™ RED and MMP-3 Fluorogenic Substrates are avidly cleaved by MMPs, with kmt/KcatS in the range of 105-107 M-1sec-1.
  • Better kinetics allows lower substrate concentrations, avoiding inhibition by the substrate or competition with the inhibitor at the active site.
  • The ultra-strong fluorescence of OMNIMMP™ RED and MMP-3 Fluorogenic Substrates allows for substrate concentrations much lower than the Km, a condition generally desirable in inhibitor screening assays.

OMNIMMP® RED fluorogenic substrate (µM)

Figure 2: Determination of kcat/Km for MMP-1 using OMNIMMP™ RED. Km = 0.48 µM,

Percentage of inhibition (%)


Figure 3: Inhibition of ten MMPs by 1.3 µM NNGH.

 

Featured Products

Matrix metalloproteinase-1 (MMP-1) fluorometric drug discovery kit, RED 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK301-0001 96 wells 541.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase-2 (MMP-2) fluorometric drug discovery kit, RED 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK302-0001 96 wells 541.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase-3 (MMP-3) fluorometric drug discovery kit, GREEN 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK303-0001 96 wells 541.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase-3 (MMP-3) fluorometric drug discovery kit, RED 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK311-0001 96 wells 525.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase-7 (MMP-7) fluorometric drug discovery kit, RED 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK304-0001 96 wells 541.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase-8 (MMP-8) fluorometric drug discovery kit, RED 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK305-0001 96 wells 541.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase-9 (MMP-9) fluorometric drug discovery kit, RED 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK306-0001 96 wells 541.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase-12 (MMP-12) fluorometric drug discovery kit, GREEN 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK312-0001 96 wells 525.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase-19 (MMP-19) fluorometric drug discovery kit, RED 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK307-0001 96 wells 525.00 USD
Do you need bulk/larger quantities?
 

Matrix metalloproteinase (MMP) inhibitor profiling kit, fluorometric RED 

Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK308-0001 96 wells 964.00 USD
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