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Quantify Difficult-to-Detect Analytes

ELISAs based on AMP'D® Technology


  • Easily improve the sensitivity of your
    sandwich ELISAs
  • AMP'D® ELISA Signal Amplification Kit
  • AMP'D® HSP70 High Sensitivity ELISA Kit

Enzo Life Sciences' AMP'D® Technology

Increase ELISA Sensitivity with the Signal Amplification AMP'D® Technology

The AMP'D® ELISA Signal Amplification system is designed to replace traditional alkaline phosphatase (AP) substrates, such as pNPP (p-Nitrophenyl phosphate), with a combination substrate and amplifier system that results in greater sensitivity when compared to a traditional substrate ELISA.

In a conventional detection system, enzyme bound to the microtiter plate interacts directly with the substrate producing a color change where the resulting absorbance is directly proportional to the amount of captured analyte. In the Amp’d ELISA system, bound AP converts a substrate that is utilized in a second enzyme reaction system which is initiated by addition of the amplifier reagent. It is this amplification step that allows for greater (amplified) color production at lower analyte concentrations resulting in an increase in assay sensitivity.

In the Amp’d ELISA system, the critical substrate component, NADPH, is added to wells containing AP and the AP reduces to NADH via release of a phosphate group. This reaction is allowed to proceed for an amount of time with the accumulated NADH being proportional to the amount of analyte/bound AP-conjugate. Upon the addition of the reconstituted amplifier reagent, this first reaction is quenched and the NADH feeds a second redox enzyme system. Here diaphorase utilizes NADH to reduce the iodonitrotetrazolium salt into formazan (purple color) producing NAD+. A counter enzymatic reaction then occurs where the NAD+ is reduced to NADH while ethanol is oxidized to acetaldehyde via alcohol dehydrogenase. This set of enzymatic reactions is also allowed to proceed for a period of time, recycling the NADH thus amplifying the original AP/substrate reaction. The resulting color intensity is ultimately proportional to the amount of bound analyte.

 

AMP'D® ELISA Kits

AMP'D® ELISA Signal Amplification Kit

AMP'D® ELISA kit provides increase in sensitivity over traditional ELISAs while detecting lower concentrations of target in samples

  • Increase sensitivity up to 50-fold over traditional ELISA
  • Quantify difficult-to-detect analytes
  • Minimal additional time needed to complete amplification step
  • Flexible format for use with any immunometric, sandwich ELISAs where greater sensitivity is desired
  • Convenient one or five 96-well plate sizes for high throughput analysis
  • Easy-to-use, simple procedure with nominal ELISA protocol modification
Amp'd™ HSP70 high sensitivity ELISA kit

Enhanced Signal Over Traditional ELISAs



Alkaline phosphatase titration of pNPP substrate versus signal amplification showing more the increase in sensitivity is observed with the new AMP'D ELISA kit (Prod. no. ENZ-KIT-100).

 

AMP'D® HSP70 High Sensitivity ELISA Kit

AMP'D® ELISA kit enabling the ability to use less sample and detect both baseline and upregulated levels of Hsp70 (Hsp72)

  • Quantify as little as 7 pg/ml of Hsp70 (Hsp72)
  • Detect both baseline and upregulated/stressed levels of Hsp70 in human, mouse and rat serum and plasma samples
  • Negligible reactivity from similar Hsp70 family members (Hsc70/Hsp73, Grp78, DnaK, or Hsp71)
  • High throughput format with results in 4.5 hours for up to 38 samples in duplicate
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
Amp'd® HSP70 high sensitivity ELISA kit

Baseline Level Detection with AMP'D® HSP70 ELISA



The new AMP'D detection system results in greater sensitivity which allows for a more reliable analysis of samples with low endogenous HSP70 concentrations.

 

AMP'D® GLP-1 High Sensitivity ELISA Kit

AMP'D® ELISA kit enabling the ability to use less sample to detect Glucagon-like peptide 1 in 3 hours.

  • Quantify as little as 5.5 pg/ml of GLP-1
  • Negligible reactivity from similar GLP-1 forms
  • High throughput format with results in 3 hours for up to 38 samples in duplicate
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
AMP'D™ GLP-1 High Sensitivity ELISA Kit



 

AMP’D® Certified kit and supporting reagents

The standard Enzo APP ΔC31 ELISA kit can be AMP'D® using an AP-conjugate antibody and our AMP'D™ detection system (AMP'D® ELISA Signal Amplification Kit). Results show up to ~10-fold increase in sensitivity (from 0.92 pM to 0.094 pM) which will allow for more reliable analysis of samples with low concentrations of APP ΔC31. We also offer Goat anti-rabbit IgG, pAb (AP conjugate) and Streptavidin (AP Conjugate) as accessory products for use with AMP’D® Technology.

 

APP ΔC31 ELISA kit 

First-to-market, sensitive ELISA kit to quantify APP ΔC31, an important amyloid precursor protein fragment with a unique pro-apoptotic mechanism leading to Alzheimer's disease.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-227-0001 96 wells 525.00 USD
Do you need bulk/larger quantities?
 

Goat anti-rabbit IgG, polyclonal antibody (AP conjugate) 

ELISA | Print as PDF
 
ENZ-ABS257-5000 5 ml 37.00 USD
Do you need bulk/larger quantities?
 

Streptavidin (AP Conjugate) 

Native. Purified from Streptomyces avidinii (SA) and calf intestine (AP)., ELISA | Print as PDF
 
ENZ-PRT119-1000 1 ml 65.00 USD
Do you need bulk/larger quantities?
 

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