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ELISA Immunoassays


Immunoassay (ELISA) Kits

Detect Small And Large Analytes Accurately & Efficiently

Enzo Life Sciences offers hundreds of ELISA kits in both immunometric and competitive assay formats. As scientists and manufacturers of kits, we understand the critical nature of your research. Each enzyme-linked immunosorbent assay is put through rigorous testing to ensure high precision, accuracy, sensitivity, and specificity. You can be confident that you will obtain reproducible results, day-after day and lot-after-lot.

Why use our ELISA assays?

  • Functional - validated in multiple sample matrices from serum and saliva to urine and feces
  • Specific - low reactivity with structurally related molecules
  • Sensitive - better quantification thresholds measure low endogenous levels with ease
  • Flexible - adaptable to automated equipment for higher throughput systems

Entrust your precious samples, time, and research budget to ELISA assay kits from Enzo Life Sciences

Assay kits can be convenient time-saving tools to help accelerate your research, but not all assays are created equal. Some that promise convenience fail to deliver biologically relevant sensitivity, or the reproducibility needed for long-term studies. This can cost you more time and money in the end. Whether you use an ELISA assay for biomarker detection, assess cellular function with fluorescent probes, or screen enzyme modulators with activity assays, every Enzo assay kit includes something a lot of other companies out there today can’t -- Experience.

It takes more than just an antibody pair to make an ELISA assay. Building an immunoassay requires screening multiple antibodies, selection of appropriate standards and conjugates, establishing proper sample prep protocols, and validation of the assay in relevant matrices. Our expertise in developing ELISAs is further backed by two decades of manufacturing excellence. Strict validation criteria and state-of-the-art manufacturing facilities deliver reproducible assays that continue to be cited in peer-reviewed publications by scientists around the world.

Reliable Manufacturing Ensures Consistent Results

cyclic AMP ELISA Assay standard curves

The graph above demonstrates the robust nature of our assays, showing standard curves for 7 lots of our competitive ELISA for cyclic AMP manufactured over 3 years.


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Quantify difficult-to-detect analytes

Reliable detection of analytes that are present at low concentrations is a critical need for early diagnosis and treatment of various diseases. Enzo Life Sciences has developed a highly sensitive ELISA Amplification System to be used with your most difficult-to-detect markers.

  • Increase sensitivity up to 50-fold over traditional ELISA
  • Minimal additional time needed to complete amplification step
  • Flexible format for use with any immunometric, sandwich ELISAs where greater sensitivity is desired
  • Convenient one or five 96-well plate sizes for high throughput analysis
  • Easy-to-use, simple procedure with nominal ELISA protocol modification
ELISAs based on Amp’d™ Technology

BIOLIKE® Conformational ELISA Kits

The BIOLIKE® Conformational ELISA kits allow for 3-D conformational comparison, higher-order structure (HOS) characterization, between biosimilars and marketed mAb. This new technology provides a sensitive, systematic, and robust measurement of biosimilar conformation comparability at the molecular level.

  • Unique measurement of biosimilar mAb HOS Comparability
  • Detects small amounts of conformational impurity, ≥ 0.1%
  • Correlates well with bioassays and glycosylation analysis

  • Featured ELISA Assay Kits

    Bioprocess ELISA Kits
    Sensitive ELISA Assays for measuring PEGylated Proteins and detecting residual Protein A contaminant.

    Cyclic Nucleotides ELISA Kits
    The most sensitive colorimetric ELISA Assays for detection of cAMP and cGMP, key second messenger signaling molecules.

    Inflammation ELISAs
    Over 100 sensitive ELISAs for immunology & inflammation research.

    Heat Shock Response ELISA Kits
    The definitive collection of Stressgen® ELISA Assays for heat shock.

    Oxidative Stress ELISA Kits
    Detect & Quantify Biomarkers of Oxidative Stress

    Toxicology ELISA Kits
    Biomarker assays for assessment of response to toxic compounds.

    Steroid / Hormone ELISA kits
    Sensitive ELISAs for the detection of steroid and hormones.

    Wnt Pathway ELISA Kits
    Assays for critical Wnt signaling components GSK-3beta, Dkk-1, and β-catenin.

    APP ΔC31 ELISA kit

    This first-to-market, complete immunoassay kit allows the quantitation of APP ΔC31 in cell lysate, cerebral spinal fluid (CSF), serum and plasma samples with results in less than two hours. APP ΔC31, is an important amyloid precursor protein fragment with a unique pro-apoptotic mechanism, produced after caspase-mediated cleavage that also releases the cytotoxic C31 fragment (AβPP-C31) after interaction with the amyloid-βprotein (Aβ) peptide. C31 and APP ΔC31 modulate death signaling pathways leading to Alzheimer's disease.

    The assay was developed in collaboration with the Buck Institute for Research on Aging and with the support of a grant from the NIA (grant # AG041456). Researchers at the Buck Institute have validated use of the assay in drug screening applications for caspase inducers and caspase inhibitors

    • Highly sensitive assay, measuring as little as 0.92 pM of APP ΔC31
    • Negligible reactivity to similar APP isoforms
    • High throughput format with results in 2 hours for up to 38 samples in duplicate
    • Fully quantitative results that surpass semi-quantitative Western blot analysis
    ELISA Assay Standard Curve

    Oxytocin ELISA kit

    The most sensitive, specific and flexible alternative to more costly and time-consuming methods for oxytocin detection.

    Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg8-Vasopressin, maintain renal water and sodium balance.

    • Sensitive quantification of 15pg/mL oxytocin
    • Negligible reactivity with vasopressin provides confidence in assay results
    • Faster and less costly than LC/MS
    • Widely published in peer reviewed literature
    ELISA Assay Standard Curve

    Featured Application

    Wildlife Endocrinology - Enzo’s trusted steroid hormone ELISA kits deliver sensitivity and reproducibility

    With thousands of species each year listed as endangered or threatened, the work done by biologists around the world to understand and preserve animal health is never ending. Reproduction studies and stress are two of the most common concerns today. Hormone analysis continues to be widely accepted for monitoring stress response and evaluating reproductive health on wildlife. Enzo Life Sciences provides a broad range of immunoassay kits for monitoring stress, reproductive physiology, health and welfare, and survival of wildlife animals.

    Featured ELISA Assays

    Stress Monitoring: Corticosterone ELISA Kit

    Reproductive Studies: Testosterone ELISA Kit


    The Basics of ELISA Immunoassays

    Understanding the basic principles of immunoassays is easy. The essential components of antibody-based immunoassay systems are threefold: an antigen to detect and perhaps quantitate; a specific antibody to this antigen; and a system to measure the amount of antigen in a given sample. Although it appears to be a very simple system, in many cases a number of other assay materials are necessary to allow for quick and convenient measurement.

    Immunometric/Sandwich ELISA protocol overview

    Sandwich ELISA protocol overview

    Immunometric assays, also known as sandwich ELISAs (enzyme-linked immunosorbent assay), use two antibodies specific to the antigen to capture or "sandwich” antigen in the well for detection. Immunometric assays exhibit a direct correlation between antigen concentration and substrate response. Immunometric assays typically employ a "capture” antibody coated on the plate to bind the antigen of interest. During a second incubation, the antigen is bound by a second "detection” antibody that is also specific to the antigen. The detection antibody can either be bound by a secondary antibody-enzyme conjugate, or the detection antibody itself is enzyme-conjugated. When chromogenic substrate is added to the assay to develop color, samples with high antigen concentration generate more signal than those with low antigen concentration, producing a signal directly proportional to the amount of antigen in the sample. This correlation can then be used to extrapolate the concentration of antigen in an unknown sample from a standard curve.

    Featured Literature

    Immunoassay Kits

    Competitive ELISA protocol overview

    Competitive ELISA protocol

    In competitive enzyme immunoassays (EIA) the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a reporter enzyme. This produces an inverse relationship between antigen concentration and substrate turnover. Competitive EIAs typically use a single antibody to a small molecular weight antigen, generally less than 10,000 Daltons. During incubation, samples with high antigen content result in unlabeled antigen being bound in greater amounts than conjugated antigen. When chromogenic substrate is added to the assay to develop color, samples with high antigen concentration generate a lower signal than those containing low antigen concentration, yielding the inverse correlation between antigen concentration in the sample and color development in the assay. This relationship can then be used to extrapolate antigen concentration in an unknown sample from a standard curve. This type of reaction is one of the few methods possible for small molecular weight antigens, such as steroids, drugs, lipids and peptides.


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