Analyze limiting quantities of total RNA with the BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System for transcript analysis
- Generates sufficient aRNA for standard microarray analysis from as little as 20 ng of total input RNA.
- Entire amplification reaction can be performed in a single tube.
- Superior 3’/5’ transcript ratios demonstrating efficient in vitro transcription.
- Biotin-labeled aRNA can be purified using either magnetic beads or purification columns and reagents
BIOARRAY™ 3´-OH Terminal Labeling is the recognized benchmark standard for biotin-labeling of DNA
The kit is considered the gold standard end-labeling system for use with Affymetrix® DNA SNP (single nucleotide polymorphism), resequencing and prokaryotic microarrays. This method uses Bio-ddUTP and terminal deoxynucleotide transferase to catalyze the addition of a single biotin-ddUMP (2’,3’-dideoxyuridine 5’-monophosphate) to the 3’-OH terminus of an amplified and fragmented target DNA molecule.
References:
- Akiyama, M et.al. Analysis of telomerase activity and RNA expression in a patient with acute promyelocytic leukemia treated with all-trans retinoic acid. Pediatric Blood & Cancer. Volume 46, Issue 4, 2006, 506-511.