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RNA Amplification & Labeling

BIOARRAY™ Amplification & Labeling Systems

Improved data quality and analysis results through greater biotin incorporation with the Single Round RNA Amplification and Biotin Labeling System for transcriptional analysis

Biotin-labeled antisense RNA (aRNA) is generated from total cellular RNA samples in less than 24 hours. Incorporation of two biotin nucleotides yields brighter signal, improving data from microarray experiments.

  • Convenient workflow with a flexible 4-16 hour transcription time and reagents supplied in a ready-to-use format.
  • Decrease experimental variation, and standardize data derived from microarrays.
  • Maintain the value of legacy data by the continued use of the gold standard for GeneChip® visualization.
  • Enables correlation of results from experiment-to-experiment, project-to-project and lab-to-lab.
  • Production of large amounts of biotin-labeled RNA targets by in vitro transcription from bacteriophage T7 RNA polymerase promoters is available separately with our BioArray HighYield® RNA Transcript Labeling Kits.

…our results indicate that
the Enzo kit is the best choice
for routine Affymetrix GeneChip experiments.

 

References:

  1. Rosen, MB et.al. Gene Expression Profiling in Wild-Type and PPARα-Null Mice Exposed to Perfluorooctane Sulfonate Reveals PPARα-Independent Effects. PPAR Research Volume 2010, Article ID 794739, doi:10.1155/2010/794739.
  2. Gabelt, BT et.al. Caldesmon effects on the actin cytoskeleton and cell adhesion in cultures HTM cells. Experimental Eye Research. Volume 82, issue 6, June 2006, 945-958.

Analyze Limiting Quantities of Total RNA

Analyze limiting quantities of total RNA with the BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System for transcript analysis

  • Generates sufficient aRNA for standard microarray analysis from as little as 20 ng of total input RNA.
  • Entire amplification reaction can be performed in a single tube.
  • Superior 3’/5’ transcript ratios demonstrating efficient in vitro transcription.
  • Biotin-labeled aRNA can be purified using either magnetic beads or purification columns and reagents


BIOARRAY™ 3´-OH Terminal Labeling is the recognized benchmark standard for biotin-labeling of DNA

The kit is considered the gold standard end-labeling system for use with Affymetrix® DNA SNP (single nucleotide polymorphism), resequencing and prokaryotic microarrays. This method uses Bio-ddUTP and terminal deoxynucleotide transferase to catalyze the addition of a single biotin-ddUMP (2’,3’-dideoxyuridine 5’-monophosphate) to the 3’-OH terminus of an amplified and fragmented target DNA molecule.

References:

  1. Akiyama, M et.al. Analysis of telomerase activity and RNA expression in a patient with acute promyelocytic leukemia treated with all-trans retinoic acid. Pediatric Blood & Cancer. Volume 46, Issue 4, 2006, 506-511.

As little as 20 ng total RNA input produces sufficient amounts of aRNA for microarrays



Figure 1: Universal Human Reference RNA ranging from 20ng to 500ng was amplified in triplicate using BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System. The lowest amount of input (20ng) generated enough labeled aRNA for microarray analysis.

 

Featured Products

BIOARRAY™ Single-round RNA amplification and biotin labeling system 

Improved data quality and  analysis results through greater biotin incorporation.
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ENZ-42420-10 10 Reactions 805.00 USD
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BIOARRAY™ Single-round RNA amplification and biotin labeling system (100 reaction) 

Improved data quality and analysis results through greater biotin incorporation.
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ENZ-42421-100 100 Reactions 6,195.00 USD
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BIOARRAY HIGHYIELD® RNA transcript labeling kit (T7) 

The gold standard in transcript labeling, for use with Affymetrix® GeneChip® microarrays.
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ENZ-42655-10 10 Reactions 845.00 USD
 
ENZ-42655-40 40 Reactions 2,645.00 USD
 
ENZ-42655-100 100 Reactions 4,955.00 USD
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BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System 

Lower RNA Input with High Yield – High Quality Biotin-labeling
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ENZ-42422-0010 10 Reactions 758.00 USD
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BIOARRAY™ Terminal labeling kit with biotin-ddUTP for DNA probe array assays 

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ENZ-42630 25 Reactions 425.00 USD
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BIOARRAY™ Eukaryotic hybridization controls 

Biotin-labeled transcripts for microarray internal controls
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ENZ-42661-30 30 Reactions 315.00 USD
 
ENZ-42661-150 150 Reactions 1,380.00 USD
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BIOARRAY™ Deoxyoligo B2 

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ENZ-42662-30 30 Reactions 75.00 USD
 
ENZ-42662-150 150 Reactions 358.00 USD
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