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AMPIPROBE® Nucleic Acid Detection

A Low-Cost Alternative For Nucleic Acid Detection

Enzo’s mission is to provide low-cost alternative solutions to the diagnostic market. Our AMPIPROBE® platform uses novel PCR design to enable the quantification of nucleic acids for clinically relevant targets. Incorporating our IP into the AMPIPROBE® assays allows Enzo to offer a low-cost option for nucleic acid detection in a price sensitive market.

AMPIPROBE® assays are easily adaptable for laboratory use and cost-effective, without compromising on quality and performance. Compatible on open PCR platforms, AMPIPROBE® assays can be validated on existing instrumentation, eliminating the need for capital expenditures.


 

Enzo's AMPIPROBE® Technology

AMPIPROBE® is a proprietary technology that incorporates probe detection in primer design. It employs a combination of fluorescent reporter-labeled primers and quencher-labeled primers to amplify DNA, akin to traditional qPCR.

When free in solution, fluorescent primers generate a signal. However, as the primers are incorporated into amplified DNA, the quencher and the fluorophore are brought within close proximity and exhibit Forster resonance energy transfer (FRET). This causes a logarithmic decay of signal with respect to the number of amplification cycles of DNA.

Once the signal decays to a defined threshold, a value is generated with respect to the corresponding cycle. The threshold cycle is indicative of the amount of target RNA or DNA in the sample.

The AMPIPROBE® HCV assay kit is Enzo’s landmark AMPIPROBE® assay for the quantitation of HCV viral load. It has been validated and approved by the New York State Department of Health to be used as a laboratory developed test to quantitatively measure HCV viral load.

 

AMPIPROBE® HCV Assay Kit

The AMPIPROBE® HCV assay kit is Enzo’s landmark AMPIPROBE® assay for the quantitation of HCV viral load. It has been validated and approved by the New York State Department of Health to be used as a laboratory developed test to quantitatively measure HCV viral load.

Key features:

  • Sensitive quantitation of HCV viral load
    • LOD Serum = 5.5 IU/mL
    • LOD Plasma = 7.9 IU/mL
  • Low-cost alternative to other methods of viral load detection
  • Compatible with most open qPCR platforms
  • Complete set of controls including:
    • High, Medium and Low quantitation controls
    • Internal sample extraction control
    • Negative sample control
  • Smaller sample input allows remaining extracted samples to be used in other tests
 

AMPIPROBE® HCV assay kit 

Sensitive and unique qPCR assay for  quantifying HCV viral load
qPCR | Print as PDF
 
ENZ-GEN200-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

Read about the validation of the AMPIPROBE™ HCV assay kit for the New York State Department of Health

Featured Literature

Scientific Posters
AMPIPROBE® A Novel, High-Sensitivity, Quantitative Hepatitis C Virus Assay
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AMPIPROBE® HCV Assay Kit
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