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AMPIGENE®
PCR & qPCR Solutions

What’s the difference? PCR vs qPCR vs RT-PCR

PCR (polymerase chain reaction) is a widely used technique to amplify/replicate DNA. The DNA that is replicated by the DNA polymerase is itself used for the next template for replication. This is the chain reaction that allows the initial DNA template (a single or few copies of a piece of DNA) to be exponentially amplified. Standard PCR uses methods to detect PCR amplification at the endpoint of the PCR reaction.

qPCR, also called real-time PCR, incorporates either dyes or probes to allow for the detection of PCR amplification during the actual reaction. More sensitive than standard PCR, qPCR measures the exponential phase of PCR for more accurate quantitation.

RT-PCR (reverse transcription – polymerase chain reaction) uses mRNA as the input sample. A reverse transcriptase enzyme is used to generate cDNA from the RNA template. Subsequently, the cDNA is used as a template for either PCR or qPCR.

Related Research Areas


 

A Complete Range of Solutions

AMPIGENE® Taq Mixes and DNA Polymerases

Our AMPIGENE® Taq Mixes and DNA Polymerases all include stabilizers, enhancers, buffers, polymerases, dNTPs, and magnesium to enable easy standard PCR. The AMPIGENE® HS Taq Mixes and HS DNA Polymerase allow for more sample types and increase PCR sensitivity.

AMPIGENE® 1-Step RT-PCR Kit

Convenient and easy, our AMPIGENE® 1-Step RT-PCR Kit has all the components for efficient cDNA synthesis and PCR in one tube. The MMLV RTase is thermostable and extremely active, allowing efficient cDNA synthesis, while the buffer contains enhancers to prevent the formation of primers/dimers to increase sensitivity and specificity.

AMPIGENE® qPCR Mixes

Using the latest developments in polymerase technology and buffer chemistry, AMPIGENE® qPCR Green Mixes and AMPIGENE® qPCR Probe Mixes require minimal optimization to deliver high performing real-time PCR. For ease of use, the qPCR Mixes come premixed with a range of different ROX reference dye intensities in order to suit your qPCR instrumentation.

AMPIGENE® qPCR 1-Step Kits

Our AMPIGENE® qPCR 1-Step Kits contain components needed for convenient cDNA synthesis and qPCR in one tube. The MMLV RTase is optimized for efficient cDNA synthesis while the qPCR components allow sensitive quantitation. Choose the convenient AMPIGENE® qPCR 1-Step Kit in the optimized reference dye format for your qPCR needs.

Higher Yield



450bp fragment of the human myc gene was amplified with AmpiGene® Taq DNA Polymerase and compared with Taq Polymerases from other suppliers. A serial dilution of human genomic DNA was incubated under standard PCR conditions. AmpiGene® Taq delivers higher yield and sensitivity as compared with all six competing products.

Higher Sensitivity



Human Genomic DNA 100ng/µl, then 1/3 serial dilution A) β2MG (Beta-2 microglubulin) gene B) pgk gene. AmpiGene® HS Taq DNA Polymerase does not produce primer-dimers and can amplify lower concentrations of DNA even from genes such as pgk that have high secondary structure levels.

Instrument Compatibility of AMPIGENE® qPCR Products

AMPIGENE® qPCR Probe Mixes and 1-Step Kits are available in Lo-ROX, HI-ROX, and No-ROX.
AMPIGENE® qPCR Green Mixes and 1-Step Kits are available in Lo-ROX and HI-ROX.

Manufacturer Instruments Lo-ROX Hi-ROX No-ROX
Analytica Jena qTower Yes Yes Yes
Applied Biosystems 7500, 7500 FAST, Viia7™ Yes No Yes
AppliedBiosystems 7000, 7300, 7700, 7900, 7900 HT, 7900 HT FAST, StepOne™, StepOne™ Plus No Yes No
Bio-Rad® iCycler®, MyIq®, iQ™5, Opticon™ CFX96™, CFX384™ Yes No Yes
Cepheid® Smartcycler® Yes Yes Yes
Eppendorf Mastercycler® ep realplex, Mastercycler® realplex 2S Yes Yes Yes
Illumina® Eco™ Yes Yes Yes
Qiagen/corbett Rotor-Gene™ 3000, 6000, Q Yes Yes Yes
Roche Applied Science Lightcycler® 480, Lightcycler® Nano Yes Yes Yes
Stratagene (Agilent) MX4000P®, MX3000P®, MX3005P® Yes No Yes
Takara Cycler Dice® Yes Yes Yes
Techne Quantica® Yes Yes Yes

AMPIGENE® DNA Ladders

Ready to use DNA standards for quantifying PCR yield

  • Ready to use format can be loaded straight onto a gel
  • Evenly spaced bands with intensified reference bands
  • Room temperature stable
 

CYGREEN® Nucleic Acid Dye

Nucleic acid dye for gel staining and higher sensitivity qPCR

CYGREEN® Nucleic Acid Dye is a DNA intercalating agent that is used to stain DNA. The DNA-dye complex emits a fluorescence spectra that makes it suitable for qPCR and gel staining applications. CYGREEN® Nucleic Acid Dye has excitation/emission spectra similar to commonly used qPCR dyes and can be substituted in to increase sensitivity and reduce cost.

  • Higher sensitivity allows detection of low target concentration
  • Greater PCR efficiency for accurate and reproducible results
  • Soluble in water for higher stability
  • Compatible with various enzyme systems
  • Suitable for commonly used rotor-type and plate-type qPCR platforms
  • Validated for qPCR applications and gel staining
  • Lower cost per assay compared to commonly used dyes


CYGREEN® Nucleic Acid Dye has higher sensitivity than competitor dyes.



CYGREEN® Nucleic Acid Dye and 4 other competitor dyes were used in qPCR reactions with varying target concentrations.

 

EQ Quencher Dyes for FRET Applications

EQ quencher dyes are suitable as acceptors in fluorescence resonance energy transfer (FRET) applications due to their broad visible absorption but no detectable fluorescence emission.




Normalized absorbance of EQ dyes showing their range of quenching through FRET.

 

DNA Decontamination Solutions

The PCR Decontamination Kit removes DNA contamination from PCR mastermixes. Contaminating DNA can be removed in the presence of primers and probe by utilizing a specific dsDNase. The dsDNase is irreversibly inactivated by heating, leaving the mastermix components unaffected.

  • Reduce background to improve target detection
  • No effect on PCR sensitivity
  • Easy protocol – decontaminate directly in mastermix

The gDNA Removal Kit removes DNA contamination from RNA prior to reverse transcription.  It uses a heat labile dsDNase which is irreversibly inactivated at low temperatures. This enables an inactivation step which is gentle enough to preserve both quality and quantity of all present RNA.

  • Removes gDNA from RNA prior to reverse transcription in the same tube
  • HL-dsDNase is inactivated without reducing quality or quantity of RNA
  • Minimizes pipetting steps and suitable for high throughput experiments

Efficient removal of gDNA without affecting RNA



The gDNA Removal Kit removes gDNA from RNA preps to levels below the detection limit but does not affect RNA. Compared to untreated control, no reduction in cDNA level (SDHA) was observed RT-qPCR.

PCR Clean-up

PCR & Gel clean-up columns use silica membrane technology in a mini spin column format to deliver easy and efficient DNA purification from PCR products, agarose gels, and labeling reactions.

  • High recoveries for fragments down to 50 bp
  • Small elution volume down to 15 µl
  • Binding buffer with pH indicator

Shrimp Alkaline Phosphatase, (recombinant), is a heat labile alkaline phosphatase that can be used in a PCR clean-up application to dephosphorylate nucleotides. It is fully inactivated by a short heat treatment to simplify workflows.

  • Completely heat inactivated after 5 min at 65°C
  • Highly stable at room temperature and 4°C

Rapid and complete heat inactivation



Heat inactivation of Shrimp Alkaline Phosphatase at 65°C and 75°C

Featured Products

AMPIGENE® 1-Step RT-PCR Kit 

Optimized and  efficient cDNA synthesis and PCR in a single tube
PCR | Print as PDF
 
ENZ-KIT105-0050 50 Reactions 180.00 USD
 
ENZ-KIT105-0200 200 Reactions 648.00 USD
 
ENZ-KIT105-1000 1000 Reactions 2,592.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® cDNA Synthesis Kit 

Efficient and sensitive cDNA synthesis for downstream  qPCR
Print as PDF
 
ENZ-KIT106-0050 50 Reactions 205.00 USD
 
ENZ-KIT106-0200 200 Reactions 738.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® Taq Mix 

Optimized standard PCR mix for enhanced speed, yield, and  specificity
PCR | Print as PDF
 
ENZ-NUC100-0040 40 Reactions 35.00 USD
 
ENZ-NUC100-0200 200 Reactions 100.00 USD
 
ENZ-NUC100-1000 1000 Reactions 400.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® HS Taq Mix 

Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed,  yield, and specificity
PCR | Print as PDF
 
ENZ-NUC101-0040 40 Reactions 45.00 USD
 
ENZ-NUC101-0200 200 Reactions 110.00 USD
 
ENZ-NUC101-1000 1000 Reactions 440.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® dNTP Mix  

Ultra-pure, stable,  and suitable for a variety of PCR applications
≥99%, PCR | Print as PDF
 
ENZ-NUC102-0500 500 µl 45.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Green Mix Lo-ROX 

Optimized dye-based  qPCR
PCR | Print as PDF
 
ENZ-NUC103-0200 200 Reactions 120.00 USD
 
ENZ-NUC103-1000 1000 Reactions 480.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Green Mix Hi-ROX 

Optimized  dye-based qPCR
PCR | Print as PDF
 
ENZ-NUC104-0200 200 Reactions 120.00 USD
 
ENZ-NUC104-1000 1000 Reactions 480.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Probe Mix Lo-ROX 

Optimized  probe-based qPCR
PCR | Print as PDF
 
ENZ-NUC105-0200 200 Reactions 95.00 USD
 
ENZ-NUC105-1000 1000 Reactions 380.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Probe Mix Hi-ROX 

Optimized  probe-based qPCR
PCR | Print as PDF
 
ENZ-NUC106-0200 200 Reactions 95.00 USD
 
ENZ-NUC106-1000 1000 Reactions 380.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Probe Mix No-ROX 

Optimized probe-based  qPCR
PCR | Print as PDF
 
ENZ-NUC107-0200 200 Reactions 95.00 USD
 
ENZ-NUC107-1000 1000 Reactions 380.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Green Kit Lo-ROX 

Optimized and efficient cDNA synthesis  and dye-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC108-0200 200 Reactions 250.00 USD
 
ENZ-NUC108-1000 1000 Reactions 1,000.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Green Kit Hi-ROX 

Optimized and efficient cDNA synthesis and dye-based qPCR in  a single tube
PCR | Print as PDF
 
ENZ-NUC109-0200 200 Reactions 250.00 USD
 
ENZ-NUC109-1000 1000 Reactions 1,000.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX 

Optimized and efficient cDNA synthesis and probe-based qPCR in a  single tube
PCR | Print as PDF
 
ENZ-NUC110-0200 200 Reactions 250.00 USD
 
ENZ-NUC110-1000 1000 Reactions 1,000.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX 

Optimized and efficient cDNA  synthesis and probe-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC111-0200 200 Reactions 250.00 USD
 
ENZ-NUC111-1000 1000 Reactions 1,000.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Probe Kit No-ROX 

Optimized  and efficient cDNA synthesis and probe-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC112-0200 200 Reactions 250.00 USD
 
ENZ-NUC112-1000 1000 Reactions 1,000.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® Taq DNA Polymerase 

Optimized standard PCR for enhanced speed, yield, and  specificity
PCR | Print as PDF
 
ENZ-PRT100-0500 500 U 105.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® HS Taq DNA Polymerase 

Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and  specificity
PCR | Print as PDF
 
ENZ-PRT101-0500 500 U 225.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® DNA Ladder 100-1,500 bp 

Ready to use DNA standards for quantifying PCR yield
Print as PDF
 
ENZ-GEN101-0100 100 lanes 66.00 USD
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AMPIGENE® DNA Ladder 50-1,500 bp 

Ready to use DNA standards for quantifying PCR yield
Print as PDF
 
ENZ-GEN102-0100 100 lanes 92.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® DNA Ladder 250-10,000 bp 

Ready to use DNA standards for quantifying PCR yield
Print as PDF
 
ENZ-GEN103-0100 100 lanes 66.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® DNA Ladder 100-10,000 bp 

Ready to use DNA standards for quantifying PCR yield
Print as PDF
 
ENZ-GEN104-0100 100 lanes 92.00 USD
Do you need bulk/larger quantities?
 

PCR & Gel Clean-up Kit 

Quick, easy kit for purifying DNA from PCR, gels and labeling reactions for a variety of downstream applications.
Print as PDF
 
ENZ-GEN100-0020 20 tests 46.00 USD
 
ENZ-GEN100-0050 50 tests 87.00 USD
 
ENZ-GEN100-0200 200 tests 301.00 USD
Do you need bulk/larger quantities?
 

gDNA removal kit 

Rapid and complete removal of gDNA from RNA preps
Print as PDF
 
ENZ-KIT136-0050 50 Reactions 152.00 USD
 
ENZ-KIT136-0250 250 Reactions 609.00 USD
Do you need bulk/larger quantities?
 

PCR decontamination kit 

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity
Print as PDF
 
ENZ-KIT137-0100 100 Reactions 125.00 USD
Do you need bulk/larger quantities?
 

Shrimp alkaline phosphatase, (recombinant) 

Heat-labile alkaline phosphatase for dephosphorylation of DNA, RNA and nucleotides
Pandalus borealis (Northern red shrimp) origin, recombinantly produced in Pichia pastoris., Cloning | Print as PDF
 
ENZ-PRT161-1000 1000 U 125.00 USD
 
ENZ-PRT161-5000 5000 U 504.00 USD
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