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In situ Hybridization

Labeling Systems, Probes & Detection


  • Detect and Identify HPV Infection in situ

  • BIO-PROBE® Labeling Systems
  • Hybridization Reagents & Buffers
  • Detection Systems
  • Digoxigenin Labeling & Detection

  • Nick Translation System for Preparing FISH Probes
  • Fluorescent-labeled dUTPs

Labeling Systems, Probes & Detection

Enzo Life Sciences offers a complete set of solutions for in situ hybridization, providing everything you need for labeling, hybridization, and detection.

  • Label a probe with your preferred BIO-PROBE® Labeling Reagent Pack (random primed or nick translation) and deoxynucleotide pack for incorporation of Bio-16-dUTP or Fluorescein-12-dUTP.
  • Hybridize with a complete set of reagents and buffers.
  • Detect with SIMPLYSENSITIVE® or ULTRASENSITIVE® Enhanced Detection Systems using Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP) colorimetric development.

No time to make your own viral detection probes?
Choose from a selection of ready-made, high-sensitivity PATHO-GENE® HPV or BIOPROBE® Infectious Agent Probes for HPV, Adenovirus, CMV, and more. Each is developed for use with our hybridization and detection systems.
Complete detection kits are available for HPV detection. Choose PATHO-GENE® HPV Typing and Screening Assays for HPV viral DNA detection or FLOWSCRIPT® HPV E6/E7 assay for a flow cytometry based assay for the single cell detection of mRNAs for HPV oncoproteins E6 and E7.

 

ISH Virus Detection

Detect and Identify HPV & Viral DNA in situ

As a long time contributor to the HPV market, Enzo developed the first non-radioactive HPV probes for in situ hybridization detection in 1986. Today, Enzo continues to contribute to the field of HPV research. By utilizing extensive knowledge of gene expression analysis and a proficiency in novel dye chemistry for cell analysis, we have developed a new molecular platform, FLOWSCRIPT™ HPV E6/E7 assay. As a pioneer in the development of HPV detection systems, Enzo continues to bring forward innovative technologies that provide clinically relevant information to researchers studying human papillomavirus.

 

PATHO-GENE® HPV Probes and typing assays for sensitive detection of viral DNA from fresh or FFPE tissue sections

PATHO-GENE® and BIO-PROBE® HPV in situ typing assays and infectious agent probes enable detection of Viral DNA in fresh or FFPE tissue sections. Discover our convenient HPV Typing assays and specific biotin-labeled probes for HPV and a variety of common infectious agents.

Detect and Identify HPV Infection in situ


CIN/condylomata biopsy specimen infected with HPV type 16 DNA. The HPV 16/18 Probe Reagent exhibited strong nuclear staining Tissue sections were developed with (A) HRP-AEC (100X) and (B) HRP-DAB (400X) and counterstained with hematoxylin.

FLOWSCRIPT® HPV E6/E7 Assay

Flow cytometry based assay for the single cell detection of mRNAs for HPV oncoproteins E6 and E7

The assay employs a novel in situ hybridization technique utilizing a cocktail of probes specific to multiple sites within the E6 and E7 genes to ensure the detection of mRNA transcripts associated with the most prevalent high risk HPV genotypes.

Multiplex Analysis of Genotypic and Phenotypic Markers in a Single Sample


 

Labeling & Detection Systems

A complete BIO-PROBE® Random Primed or Nick Translation DNA Labeling System consists of the combination of two separate components: a Reagent Pack (labeling system) and a choice of deoxynucleotide packs for Bio-16-dUTP or Fluorescein-12-dUTP. Once labeling of choice is completed, hybridization is performed with a complete set of reagents, followed by detection with SimplySensitive® or UltraSensitive® Detection Systems.

Featured Literature

 

BIO-PROBE® Labeling Systems

BIO-PROBE® Nick Translation Systems
BIO-PROBE® Random Primed Labeling Systems

Hybridization Reagents & Buffers

ISH Detection Systems

SIMPLYSENSITIVE® In Situ Detection Systems
ULTRASENSITIVE® Enhanced In Situ Detection Systems


Digoxigenin Labeling & Detection

Digoxigenin NHS ester
Digoxigenin-dUTP, alkali-stable
Digoxigenin-UTP, alkali-stable
Digoxigenin polyclonal antibody


Modified Nucleotides & Nick Translation Labeling

Ensure Peak Labeling Performance for FISH Probe Synthesis

Fluorescent dye-dUTPs are well recognized as superior to analogous methods using cumbersome indirect two-step labeling methods. When coupled with the Nick Translation DNA Labeling System, this direct approach provides a simple and efficient method to label DNA for FISH, suitable for a wide range of molecular biology and cytogenetics applications.

  • Eight distinct colors to choose from, spanning the visible light spectrum
  • High signal intensity and good photostability






Labeling DNA probe for FISH analysis.

 

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