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Sample Conversion

High-efficiency Conversion of 5-mC and 5-hmC DNA for the Truest Downstream Analysis

  • Conversion allows for resolution of single nucleotide information
  • Kits provide high conversion rates without the introduction of DNA damage
  • Converted output sample suitable for detection by bisulfite sequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing
 
DNA Only Blood, Tissue, FFPE, DNA DNA Only
Express DNA Methylation Kit Blood & Tissue DNA Methylation Kit BioArray™ 5-hmC Methylation Kit
Target & Modification 5-mC Bisulfite modification 5-mC Bisulfite modification 5-hmC β-Glucosyltransferase protection
Format Spin Column 96-Well Spin Column 96-Well Spin Column
Elution Volume ≥ 10 μl ≥ 15 μl ≥ 10 μl ≥ 15 μl ≥ 6 μl
Conversion Efficiency >99.5% >99.5% *Enzymatic efficiency ~100%
Processing Time 1.5 hours 4 hours 6-8 hours
Input 100 pg – 2 μg of DNA ≥ 50 pg DNA, ≥ 10 cells, blood, tissue, FFPE 100 ng - 500 ng DNA

Express DNA methylation kit 

Fastest method of complete bisulfite conversion of DNA for methylation treatment
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ENZ-45001-0050 50 tests 240.00 USD
 
ENZ-45001-0200 200 tests 660.00 USD
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Express DNA methylation kit (deep well) 

Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
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ENZ-45002-0002 2x96 wells 555.00 USD
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Express DNA methylation kit (shallow well) 

Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
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ENZ-45003-0002 2x96 wells 555.00 USD
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Blood and tissue DNA methylation kit 

Complete bisulfite conversion from blood and tissue samples
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ENZ-45004-0050 50 tests 240.00 USD
 
ENZ-45004-0200 200 tests 660.00 USD
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BIOARRAY™ 5-hmC methylation kit 

Detect 5-hydroxymethylcytosine using a robust glucosylation method
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ENZ-45011-0050 50 tests 536.00 USD
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BIOARRAY™ methylated DNA IP Kit 

Enrich for methylated DNA (100-fold) from sample for complete diverse downstream applications
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ENZ-45012-0010 10 tests 575.00 USD
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