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DNA Methylation Detection

Real-Time PCR Based Detection

BIOPANEL® DNA Methylation Detection Kit for Human Pluripotent Stem Cells

  • No need for bisulfite conversion
  • Determine % methylation of specific region in less than 5 hours
  • Identifies region-specific DNA methylation in RAB25, NANOG, PTPN6, MGMT, GBP3, and LYST gene promoters
Figure: Human differentiated DNA (yellow stripped bars) and human stem cell DNA (white bars) show different DNA methylation percentages for RAB25, NANOG, PTPN6, MGMT, GBP3 and LYST
Cell Population RAB25 NANOG PTPN6 MGMT GBP3 LYST
Differentiated + + + - - -
Pluripotent - - - + + +

ELISA-Based Detection

5-Methylcytosine DNA ELISA kit

  • Accurately quantitate 5-mC in any DNA sample in less than 3 hours
  • Ideal for high-throughput analysis
  • High specificity comparable to LC-MS/MS-MRM analysis
Genomic DNA Mass Spectroscopy versus ELISA analysis: The 5-mC DNA ELISA Kit (prod. no. ADI-900-224) quantifies 5-mC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. Genomic DNA samples include: human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), and mouse testes (MT).
 

Colorimetric Assay Based Detection

DNMT Colorimetric Drug Discovery Kit

Rapid, sensitive, and simple colorimetric DNMT activity/inhibition kit.

  • Rapid - results in less than 4 hours
  • Simple and safe - Easy colorimetric assay without radioactivity or chromatography
  • Sensitive - measure as little as 0.5 μg of nuclear extract or 0.5 ng of purified enzymes
  • Specific - Optimized to allow high specificity to 5-mC without cross-reactivity to unmethylated cytosine
  • Reliable - reproducible results
 

BIOPANEL® methylation detection kit for human pluripotent stem cells (Light Cycler™ 480 RT PCR) 

Rapid, high-throughput assay for quantifying methylation to determine pluripotency state of cells using Light Cycler™ 480 RT PCR
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ENZ-45007-0001 96 wells 552.00 USD
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5-Methylcytosine DNA ELISA kit 

Streamlined, rapid ELISA kit to quantify 5-methylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-224-0001 96 wells 472.00 USD
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5-Hydroxymethylcytosine DNA ELISA kit 

Highly sensitive, rapid ELISA kit to quantify 5-hydroxymethylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-225-0001 96 wells 472.00 USD
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Universal methylated DNA standard (human) 

Methylation standard (human) to help determine efficiency of a bisulfite DNA conversion
Print as PDF
 
ENZ-45005-0001 20 tests 254.00 USD
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Universal methylated DNA standard (mouse) 

Methylation standard (mouse) to help determine efficiency of a bisulfite DNA conversion
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ENZ-45006-0001 20 tests 254.00 USD
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DNMT Colorimetric Drug Discovery Kit 

Rapid, sensitive, and simple colorimetric DNMT activity/inhibition kit.
Colorimetric detection | Print as PDF
 
ENZ-45017-0001 96 Reactions 587.00 USD
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5-Methylcytosine monoclonal antibody (10G4) 

ELISA, IP, Immunoblot | Print as PDF
 
ADI-905-903-0050 50 µg 215.00 USD
 
ADI-905-903-0200 200 µg 604.00 USD
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5-Hydroxymethylcytosine polyclonal antibody 

ELISA, IP, Immunoblot | Print as PDF
 
ADI-905-904-0050 50 µg 194.00 USD
 
ADI-905-904-0200 200 µg 604.00 USD
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