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Live Cell, Multiplex Analysis Kits

Accurately Profile Total ROS and Superoxide with Dual-readout Assay
Total ROS/Superoxide Detection Kit

  • Non-invasive fluorescent probes allow simultaneous discrimination of total ROS and superoxide in live cells
  • Profile to distinguish between hydrogen peroxide, peroxynitrite and hydroxyl radicals using included inhibitors
  • Dyes compatible with major components of tissue culture media (phenol red, FBS and BSA) for microscopy and flow cytometry
  • Red dye for detection of Nitric Oxide also available (ENZ-51013 / ENZ-51001)

Multiplex Analysis of Total ROS AND Superoxide

Data represents treatment of HeLa cells with Pyocyanin (ROS/SO inducer). Total ROS dye fluoresces green in the presence of ROS, while the SO-specific dye fluoresces yellow/orange (bottom right).

Convenient Quantifiable Flow Cytometry Assay

Data represents % of positive HeLa cells following treatment with Pyocyanin (ROS/SO inducer), TBHP (ROS Inducer), or AMA (Superoxide inducer).

 

Total ROS/Superoxide detection kit for microscopy, flow cytometry and microplates 

Directly Monitor Global Levels of Reactive Oxygen Species (ROS), and Specifically Superoxide
FC, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51010 1 Kit 224.00 USD
 

ROS/RNS detection kit for microscopy 

Fluorescent detection | Print as PDF
 
ENZ-51001-200 1 Kit 272.00 USD
 

Total ROS detection kit for microscopy and flow cytometry 

FC, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51011 1 Kit 162.00 USD
 

Superoxide detection kit for microscopy and flow cytometry 

FC, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51012 1 Kit 160.00 USD
 

NO detection kit for microscopy 

Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-51013-200 1 Kit 160.00 USD
 

Multiplex, Real-time Analysis of Hypoxia and ROS in Live Cells
NEW Cyto-ID® Hypoxia/Oxidative Stress Detection Kit

  • Sensitive hypoxia dye fluoresces red when converted by nitroreductases
  • Total ROS dye enables simultaneous detection of hypoxia (red) and ROS (green)
  • Non-toxic, cell permeable dyes optimized to avoid artifactual fluorescence
  • Suitable for microscopic or flow cytometric analysis of adherent or suspension cells

Simultaneously Monitor Hypoxic Status and ROS Formation

Quantitative Multiparametric Assay for Flow Cytometry

 

Bright red fluorescence of the Hypoxia probe is observed following its conversion by cellular nitroreductases under hypoxic conditions such as those induced chemically by treatment with the hypoxia-mimetic desferrioxamine (DFO). The assay facilitates simultaneous quantification of hypoxic status (FL3/red) and presence of ROS (FL1/green) by flow cytometry.

Cyto-ID® Hypoxia/Oxidative Stress Detection kit 

Specific detection system for simultaneous analysis of hypoxia and oxidative stress using microscopy and flow cytometry
FC, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51042-0125 125 tests 100.00 USD
 
ENZ-51042-K500 500 tests 350.00 USD
 

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