Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

DNA Damage & Content Analysis

DNA Damage ELISA Kit

The DNA Damage ELISA (enzyme-linked immunosorbent assay) is a fast and sensitive immunoassay providing results in less than 2.5 hours. Quantitation of 8- hydroxy-2’-deoxyguanosine (8-OHdG) in urine, serum, and saliva samples is performed in a convenient 96-well plate format using a colorimetric substrate. 8-OHdG is a frequently-used critical biomarker of oxidative stress and carcinogenesis.

  • Quantify levels < 1 ng/ml
  • Validated in-house in a variety of sample matrices
  • Tested in a variety of biofluids (urine, serum, and saliva)
  • Convenient colorimetric 96-well plate format

Typical 8-OHdG Standard Curve

Figure 1: The standard curve has a range of 0.94 – 60 ng/mL.
 

COMET SCGE Assay Kit

Sensitive and versatile method for measuring single- and double-strand DNA breaks in individual cells.
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage. The Assay Designs COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic “comet” shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

The COMET SCGE Assay is a fast and simple electrophoresis method to detect and quantitate DNA fragmentation in cells associated with DNA damage and apoptosis. A unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide.

  • Ready-to-use Comet Slides allow direct application of sample without pretreatment
  • Shorter assay time allows for higher throughput sample analysis
  • Hydrophobic barrier allows sample treatment with DNA repair enzymes
  • Unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide

NUCLEAR-ID® Cell Cycle Analysis Kits

Highly permeable fluorescent dyes for DNA content analysis in live or fixed cells.

  • Intercalating dye with superior permeability in live cells
  • Dye functional over a wide range of cell densities, incubation time, and temperature eliminating optimization required with other dyes
  • Easy no-wash, mix and read protocol
  • Dyes excitable with standard 488nm laser
Figure 1. G2/M accumulation of Nocodazole-treated cells using Nuclear-ID dye in live cells.
 

References:

  1. Houben R, et.al. An intact retinoblastoma protein binding site in merkel cell polyomavirus large T antigen is required for promoting growth of merkel cell carcinoma cells. Int J Cancer. 2011 Mar 16. doi: 10.1002/ijc.26076 GFP-certified® Nuclear-ID® Red Cell Cycle Analysis Kit
  2. Xiang, Y, et.al. A cell-permeant dye for cell cycle analysis by flow and laser-scanning microplate cytometry. Nature Methods 6, (2009) (Advertising Feature) Nuclear-ID® Red Cell Cycle Analysis Kit, GFP-certified®

Featured Products

Comet SCGE assay kit 

Fast and simple electrophoresis method to measure DNA fragmentation
Fluorescence microscopy | Print as PDF
 
ADI-900-166 50 tests 295.00 USD
Do you need bulk/larger quantities?
 

DNA damage ELISA kit 

Rapid DNA damage ELISA kit for cancer, apoptosis and oxidative stress.
ELISA, Colorimetric detection | Print as PDF
 
ADI-EKS-350 96 wells 765.00 USD
Do you need bulk/larger quantities?
 

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,