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Screen Wnt Pathway Activators & Inhibitors

Development of Wnt pathway modulating drugs requires the ability to rapidly screen the effect of novel or repurposed compounds and biological therapeutics on the expression of Wnt-regulated genes, and confirmation of such effects by related changes in key signaling components. Together, the LEADING LIGHT™ Wnt Reporter Assay, LEADING LIGHT™ Sclerostin-LRP Screening System and SCREEN-WELL® Wnt Pathway Library facilitate high-throughput analysis of Wnt-related signaling.

 

Reliable, All-inclusive Screening of Wnt Pathway Modulators

The LEADING LIGHTt™ Wnt Reporter Assay Starter Kit is a high-sensitivity, luciferase-based assay for the analysis of canonical Wnt signaling. The assay uses a 3T3 mouse fibroblast cell line modified with a luciferase reporter gene under the control of Wnt-responsive promoters (TCF/LEF). The chemiluminescent readout is amenable to both 96-well and 384-well plate formats, facilitating high-throughput analysis of Wnt pathway activators and inhibitors.

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Benefits

  • A true end-point detection system for screening compounds
  • Greater sensitivity than other whole cell assay kits on the market
  • Reproducibility that comes from using a validated system
  • Time-saving, no-transfection kit includes cells, media, detection reagents and controls
 

Novel Cell-free Sclerostin/LRP Binding Assay Ideal for Primary Inhibitor Screens

Enzo Life Sciences continues to expand its portfolio of Wnt Pathway assays with the NEW LEADING LIGHT™ Sclerostin-LRP Screening System. This simple, rapid biochemical binding assay format does not require cell lines or transfection reagents, making it a convenient platform for initial screening of inhibitors of the Wnt antagonist Sclerostin.

  • High sensitivity chemiluminescent readout (signal-to-noise ratio of ≥ 10)
  • Rapid protocol yields results in just 2.5 hours
  • Reproducible, high-throughput amenable assay (Z’-factor 0.871)
  • No cell lines or transfection required
 

The SCREEN-WELL® Wnt Pathway Library offers a convenient solution for benchmarking compounds against a collection of 71 known Wnt modulators. The library is available in 100uL and 500uL formats (dissolved in DMSO) for use as a companion to the LEADING LIGHT™ Wnt Reporter Assay, or individually as a general screening tool for chemical genomics or Wnt assay.

ScreenWell™ Wnt Pathway Library Targets Include
Wnt5a Dkk Axin Porcupine β-catenin LRP5/6
TCF Dishevelled GSK-3B Adenylate Cyclase COX-II sFRP-1


Application Note

Featured Products

LEADING LIGHT® Wnt Reporter Assay Starter Kit 

Chemiluminescent cell-based assay for discovering Wnt pathway modulators
FUNC, Chemiluminescence, HTS | Print as PDF
 
ENZ-61001-0001 96 wells 946.00 USD
Do you need bulk/larger quantities?
 

LEADING LIGHT® Wnt Reporter Cell Line 

Wnt reporter cell line for discovering Wnt pathway modulators
Print as PDF
 
ENZ-61002-0001 1 ml 525.00 USD
Do you need bulk/larger quantities?
 

LEADING LIGHT® Wnt Cell Line Medium Pack 

For use with the LEADING LIGHT® Wnt Reporter Cell Line
Print as PDF
 
ENZ-60003-0001 1 Pack 342.00 USD
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LEADING LIGHT® Wnt Cell Line Assay Reagents 

For use with the LEADING LIGHT® Wnt Reporter Cell Line
Print as PDF
 
ENZ-60004-0001 1 Pack 420.00 USD
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LEADING LIGHT® Sclerostin-LRP Interaction Screening System 

Novel Cell-free Sclerostin/LRP Binding Assay Ideal for Primary Inhibitor Screens
FUNC, Chemiluminescence, HTS | Print as PDF
 
ENZ-61003-0001 96 wells 380.00 USD
Do you need bulk/larger quantities?
 

SCREEN-WELL® Wnt Pathway library 

Compounds are highly relevant; many are unique
HTS | Print as PDF
 
BML-2838-0500 1 Library 500 µl/well Inquire for pricing
 
BML-2838-0100 1 Library 100 µl/well Inquire for pricing
Do you need bulk/larger quantities?
 

BML-284 

Wnt activator
853220-52-7, ≥98% (TLC) | Print as PDF
 
BML-WN105-0005 5 mg 116.00 USD
 
BML-WN105-0025 25 mg 460.00 USD
Do you need bulk/larger quantities?
 

QS11 

Wnt pathway activator
944328-88-5, ≥98% (HPLC) | Print as PDF
 
BML-WN117-0005 5 mg 132.00 USD
 
BML-WN117-0025 25 mg 553.00 USD
Do you need bulk/larger quantities?
 

BML-287 

Selective inhibitor of sFRP-1
≥98% (HPLC) | Print as PDF
 
BML-WN108-0005 5 mg 150.00 USD
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