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Toll-like Receptors

TLRGRADE™ LPS & Lipid A Reagents

Providing the purest ligands for recognition by pattern recognition receptors.

  • Ultrapure (≥99.9%)
  • Sterile, ready-to-use liquid formulation
  • No hazardous handling

Worry-Free Antibodies

Enzo offers over 50 TLR antibodies validated for flow cytometry and microscopy applications.


TLR antibodies
 

High Purity LPS & Lipid A Preparations Do Not Activate TLR2 or other TLRs


Activation of macrophages from TLR4 wild type compared to TLR4 deficient mice by TLRGRADE™ LPS and TLRGRADE™ Lipid A from Enzo Life Sciences. Lipid A or LPS concentrations, which induced maximal activation of TLR4 wild type mouse macrophages, were also applied to TLR4 deficient mouse macrophages (105) as follows: 80ng Salmonella LPS (S. minnesota R595 Re), 80ng E. coli LPS (R515 Re), 400ng E. coli Lipid A (R515 Re) and 400ng Salmonella LPS (S. abortus equi S-form). 10 units of IL-6 correspond to the detection limit of the IL-6 ELISA.

Related Products

LPS from E. coli, Serotype EH100 (Ra) (TLRgrade™) (Ready-to-Use) 

TLR4 activator
Rough (R)-form LPS isolated and purified from E. coli EH100 (Ra-mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-010-L001 1 ml 148.00 USD
 
ALX-581-010-L002 2 ml 214.00 USD
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LPS from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use) 

TLR4 activator
Rough (R)-form LPS isolated and purified from E. coli R515 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-007-L001 1 ml 148.00 USD
 
ALX-581-007-L002 2 ml 210.00 USD
Do you need bulk/larger quantities?
 

LPS from E. coli, Serotype O111:B4 (TLRgrade™) (Ready-to-Use) 

TLR4 activator
Smooth (S)-form LPS isolated and purified from E. coli O111:B4 by a modification of the phenol water extraction and PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-012-L001 1 ml 134.00 USD
 
ALX-581-012-L002 2 ml 210.00 USD
Do you need bulk/larger quantities?
 

LPS from E. coli, Serotype O55:B5 (TLRgrade™) (Ready-to-Use) 

Specific TLR4 activator
Smooth (S)-form LPS isolated and purified from E. coli 055:B5 by a modification of the phenol water extraction and PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-013-L001 1 ml 148.00 USD
 
ALX-581-013-L002 2 ml 210.00 USD
Do you need bulk/larger quantities?
 

LPS from Salmonella abortus equi S-form (TLRgrade™) (Ready-to-Use) 

TLR4 activator
Smooth (S)-form LPS, isolated and purified from Salmonella abortus equi by modification of the phenol water extraction and PCP method, converted to the uniform salt form and dissolved in pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-009-L001 1 ml 148.00 USD
 
ALX-581-009-L002 2 ml 220.00 USD
Do you need bulk/larger quantities?
 

LPS from Salmonella abortus equi S-form (TLRgrade™) (Ready-to-Use) (Biotin) 

TLR4 activator
Smooth (S)-form LPS, isolated and purified from Salmonella abortus equi by modification of the phenol water extraction and PCP method. LPS-Biotin was prepared using the biotin reagent biotinamidocaproate N-hydroxysuccinimide ester. Briefly, LPS at 10mg/ml in distilled water was mixed with biotin reagent in sodium bicarbonate buffer. The reaction mixture was stirred, dialyzed extensively against distilled water in the dark, and sterile filtered., Absence of dectectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-150-R500 500 µl 351.00 USD
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LPS from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use) 

TLR4 activator
Rough (R)-form LPS, isolated and purified from Salmonella minnesota R595 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-008-L001 1 ml 134.00 USD
 
ALX-581-008-L002 2 ml 210.00 USD
Do you need bulk/larger quantities?
 

LPS from Salmonella typhimurium S-form (TLRgrade™) (Ready-to-Use) 

TLR4 activator
Smooth (S)-form LPS, isolated and purified from Salmonella typhimurium by modification of the phenol water extraction and PCP method, converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-011-L001 1 ml 148.00 USD
 
ALX-581-011-L002 2 ml 231.00 USD
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Monophosphoryl Lipid A (synthetic) (Ready-to-Use) 

TLR4 activator
1246298-63-4, Synthetic. | Print as PDF
 
ALX-581-205-C100 100 µg 269.00 USD
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Monophosphoryl Lipid A from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use) 

TLR4 activator
Isolated from E. coli, Serotype R515 (Re mutant). Prepared by acid hydrolysis from purified E. coli, Serotype R515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) | Print as PDF
 
ALX-581-203-R500 500 µl 263.00 USD
 
ALX-581-203-L001 1 ml 394.00 USD
Do you need bulk/larger quantities?
 

Monophosphoryl Lipid A from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use) 

TLR4 activator
Isolated from Salmonella minnesota R595 (Re mutant). Prepared by acid hydrolysis from purified Salmonella minnesota R595 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) | Print as PDF
 
ALX-581-202-R500 500 µl 290.00 USD
 
ALX-581-202-L001 1 ml 394.00 USD
Do you need bulk/larger quantities?
 

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