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Prostaglandin Analysis

Enzo Life Sciences offers the most sensitive and complete colorimetric ELISA kits for quantification of prostaglandins in a wide variety of sample types.

  • Ultrasensitive colorimetric ELISAs to measure as little as 8.26 pg/ml PGE2
  • Widely cited in peer reviewed literature
  • Available to use for cell lysates, culture supernatants, serum, saliva, urine, and many more sample types
  • High throughput capabilities with chemiluminescent and fluorescent format options
  • Proven manufacturing capabilities for reliable lot-to-lot results
 

PGE2 ELISA Kits

Product Number Size Sensitivity Time to Answer Sample Types
Colorimetric ADI-900-001 96 wells 13.4 pg/mL <3 hours Culture supernatants, serum, saliva, urine, whole blood, and more
ADI-901-001 5x96 wells
ADI-930-001 96 wells 8.26 pg/mL Overnight Culture supernatants, serum, saliva, urine, whole blood, plasma
ADI-931-001 5x96 wells
Fluorescent (FPIA) ADI-920-001 100 tests 684 pg/mL 30 min Culture supernatants
Chemiluminescent (CLIA) ADI-910-001 96 wells 6.03 pg/mL 3 hours Culture supernatants, serum, saliva, urine, whole blood

PGE2 ELISA kit 

Highly sensitive PGE2 ELISA kit for inflammation and eicosanoid research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-001 96 wells 362.00 USD
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PGE2 ELISA kit 

Highly sensitive PGE2 ELISA kit for inflammation and eicosanoid research
ELISA, Colorimetric detection | Print as PDF
 
ADI-901-001 5x96 wells 1,434.00 USD
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PGE2 high sensitivity ELISA kit 

Ultrasensitive PGE2 ELISA kit for inflammation and eicosanoid research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-930-001 96 wells 380.00 USD
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PGE2 high sensitivity ELISA kit 

Ultrasensitive PGE2 ELISA kit for inflammation and eicosanoid research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-931-001 5x96 wells 1,512.00 USD
Do you need bulk/larger quantities?
 

PGE2 FPIA kit 

Fluorescence microscopy | Print as PDF
 
ADI-920-001 100 tests 259.00 USD
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PGE2 CLIA kit 

Chemiluminescence | Print as PDF
 
ADI-910-001 96 wells 378.00 USD
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Featured Prostaglandin Citations

  • W.Y. Choi, et al., 'Anti-inflammatory, antioxidative and matrix metalloproteinase inhibitory properties of 20(R)-ginsenoside Rh2 in cultured macrophages and keratinocytes', (2013) J. Pharm. Pharmacol., v.65, p.310.
  • T. Li, et al., 'Colorectal carcinoma-derived fibroblasts modulate natural killer cell phenotype and antitumor cytotoxicity', (2013) Med. Oncol., v.30, p.663.
  • Y. Mao, et al., 'Melanoma-educated CD14+ cells acquire a myeloid-derived suppressor cell phenotype through COX-2-dependent mechanisms', (2013) Cancer Res., v.73, p.3877.
  • A. Thakur, et al., 'Microenvironment generated during EGFR targeted killing of pancreatic tumor cells by ATC inhibits myeloid-derived suppressor cells through COX2 and PGE2 dependent pathway', (2013) J. Transl. Med., v.11, p.35.
  • M.S. Pour, et al., 'Prostaglandin E2 alteration in contraceptive consumers: as a risk factor for inflammatory diseases', (2013) Arch. Gynecol. Obstet., v.287, p.1031.
  • G.M. Park, et al.,'XH-14, a novel danshen methoxybenzo[b]furan derivative, exhibits anti-inflammatory properties in lipopolysaccharide-treated RAW 264.7 cells', (2013) J. Inflamm., v.10, p.1

Lot-to-lot Consistency

Lot-to-lot consistency graph demonstrates the robust and reproducible nature of the PGE2 ELISA kit (<a href="http://www.enzolifesciences.com/ADI-900-001/pge2-elisa-kit/">ADI-900-001</a>) showing standard curves from 10 lots manufactured over 5 years.

Selected Literature

Technical Posters
Arachidonic Acid Metabolites
 

Biological Significance of Prostaglandins

The prostaglandins, together with the thromboxanes and prostacyclins, are fatty acid derivatives and a subclass of eicosanoids. Like all eicosanoids, the prostaglandins contain 20 carbon atoms and function as locally acting messenger molecules. They are produced at discrete sites acting as autocrine or paracrine factors rather than endocrine hormones. In the body, they mediate a variety of strong physiological effects, such as regulating the contraction and relaxation of smooth muscle tissue.

Prostaglandin E1 (PGE1) is synthesized from DGLA, dihomo-γ-linolenic acid and has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has been shown to have insulin-like actions. PGE1s effects are induced by receptor mediated elevation of cAMP.

Prostaglandin E2 (PGE2) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. Additionally, PGE2 has been shown to be a regulator of sodium excretion and renal hemodynamics.

Prostaglandin F (PGF) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. It is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with pre-eclampsia.

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Prostaglandin F 

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13,14-Dihydro-15-keto-prostaglandin F 

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6-Ketoprostaglandin F 

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9β,11α-Prostaglandin F2 

Bronchodilator, antagonizes effects of PGF2α.
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9α,11β-Prostaglandin F2 

Prostanoid receptor ligand
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Prostanoid receptor ligand
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2,3-Dinor-6-keto-prostaglandin F-20,20,20-d3 

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17-Phenyl-trinor-prostaglandin E2 

Prostanoid receptor ligand
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15-Deoxy-Δ12,14-prostaglandin J2 

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87893-55-8, ≥97% (TLC) | Print as PDF
 
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Prostaglandin H2 

Activates platelets
42935-17-1, ≥95% (HPLC) | Print as PDF
 
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