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Cyclic Nucleotide Analysis

Biological Significance of Cyclic Nucleotides

The second messengers adenosine 3’, 5’-cyclic monophosphate (cyclic AMP; cAMP) and guanosine 3’, 5’-cyclic monophosphate (cyclic GMP; cGMP) are important intracellular regulators downstream from GPCRs.

cAMP is also involved in regulating neuronal, glandular, cardiovascular, immune and other functions. A number of hormones are known to activate cAMP through the action of the enzyme adenylate cyclase which converts ATP to cAMP. The diversity of receptors known to impact cAMP-mediated signaling includes those known to affect cardiovascular and nervous systems, immune mechanisms, cell growth, differentiation, and general metabolism. Thus, there remains considerable interest in the measurement of intracellular cAMP concentrations in tissues and cell culture.

cGMP is typically present at levels 10 to 100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. It is most well recognized as a key signaling trigger in the relaxation of smooth muscle cells (resulting in vasodilation) in response to nitric oxide, and as a sodium ion channel regulator (via its degradation by phosphodiesterases) critical to phototransduction. Vasodilators and hormones, such as acetylcholine, insulin and oxytocin, as well as serotonin and histamine, cause an increase in cGMP levels.

In addition to measuring cAMP and cGMP, detection of downstream GPCR signaling events such as activation of Erk1/2, Akt, Src and Stat3 are routinely assayed using phospho-specific antibodies to distinguish phosphorylation of signaling proteins at specific activating or inactivating serine, threonine, and tyrosine residues.

 

High Quality Cyclic Nucleotide Assays

Enzo Life Sciences offers the most sensitive and complete colorimetric ELISA kits for quantification of intracellular or extracellular cAMP or cGMP in a variety of sample types.

  • Sensitive – optional acetylation protocol increases sensitivity 10-fold (0.039 pmol/ml)
  • Trusted – regularly cited in peer-reviewed publications
  • Simple – efficient and well-established sample handling protocols
  • Reliable – consistent lot-to-lot performance
Lot-to-lot consistency: Graph demonstrates the robust and reproducible nature of the competitive cAMP ELISA kit (Prod. #ADI-900-067) showing standard curves from 10 lots manufactured over 6 years.
 

Choose the Kit that Fits Your Needs

Sample Types
intracellular Extracellular
Cell Lysates Tissue Extracts Culture Supernatants Serum Saliva Urine
Kit Type Complete Yes Yes Yes Yes Yes Yes
Direct Yes Yes
Standard Yes Yes Yes Yes

COMPLETE Kits

Include all components necessary for quantifying both intra- and extracellular cAMP or cGMP.
Sample Types: Cell lysates, tissue extracts, culture supernatants, serum, saliva, urine

cAMP complete ELISA kit 

Most sensitive (0.037 pmol/ml) and complete colorimetric kit for intra- and extracellular cAMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-163 96 wells 380.00 USD
 

cAMP complete ELISA kit 

Most sensitive (0.037 pmol/ml) and complete colorimetric kit for intra- and extracellular cAMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-901-163 5x96 wells 1,520.00 USD
 

cGMP complete ELISA kit 

Most sensitive (0.043 pmol/ml) and complete colorimetric kit for intra- and extracellular cGMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-164 96 wells 380.00 USD
 

cGMP complete ELISA kit 

Most sensitive (0.043 pmol/ml) and complete colorimetric kit for intra- and extracellular cGMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-901-164 5x96 wells 1,520.00 USD
 

DIRECT Kits

Include components for quantifying intracellular cAMP or cGMP
Sample Types: Cell lysates, tissue extracts.

Direct cAMP ELISA kit 

Most sensitive (0.037 pmol/ml) colorimetric kit for intracellular cAMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-066 96 wells 360.00 USD
 

Direct cAMP ELISA kit 

Most sensitive (0.037 pmol/ml) colorimetric kit for intracellular cAMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-901-066 5x96 wells 1,440.00 USD
 

Direct cGMP ELISA kit 

Most sensitive (0.025 pmol/ml) colorimetric kit for intracellular cGMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-014 96 wells 360.00 USD
 

Direct cGMP ELISA kit 

Most sensitive (0.025 pmol/ml) colorimetric kit for intracellular cGMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-901-014 5x96 wells 1,440.00 USD
 

STANDARD Kits

Include components for quantifying extracellular cAMP or cGMP.
Sample Types: Culture supernatants, serum, saliva

cAMP ELISA kit 

Most sensitive (0.039 pmol/ml) colorimetric kit for extracellular cAMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-067 96 wells 330.00 USD
 

cAMP ELISA kit 

Most sensitive (0.039 pmol/ml) colorimetric kit for extracellular cAMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-901-067 5x96 wells 1,320.00 USD
 

cGMP ELISA kit 

Most sensitive (0.088 pmol/ml) colorimetric kit for extracellular cGMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-013 96 wells 330.00 USD
 

cGMP ELISA kit 

Most sensitive (0.088 pmol/ml) colorimetric kit  for extracellular cGMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-901-013 5x96 wells 1,320.00 USD
 

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