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Autophagy ELISA Kits

Sensitive NBR1 and p62 ELISA Kits allow for quantitative, immunometric detection of the autophagy biomarkers NBR1 and p62 (Sequestosome 1) in human, rat and mouse cell lysates. NBR1 and p62 function as scaffold proteins, aiding in autophagy protein trafficking and degradation. ELISA kits enable quantitative measurement of autophagy without the need for expensive equipment or long procedures

  • Sensitive assays measure as little as 66 and 100 pg/ml of NBR1 and p62, respectively
  • Fully quantitative results surpass semi-quantitative Western blot analysis
  • High throughput format allows analysis of up to 40 samples in duplicate in less than 3 hours
  • Easy-to-follow protocols and liquid color-coded reagents save time and reduce errors

 

 

Human breast cancer cells were treated with either autophagy-inducing drug or vehicle. Cells were harvested 6 and 12 hours post-treatment, lysed, and analyzed with the p62 ELISA kit, and for p62 and LC3- II by Western blot. Drug treatment correlated with the induction of autophagy as indicated by the decrease in p62 levels (A and B) and by elevation of LC3-II levels (C).

 

NBR1 ELISA kit 

First-to-market, quantitative NBR1 ELISA kit available for autophagy research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-211-0001 96 wells 500.00 USD
 

p62 ELISA kit 

Highly validated, quantitative p62 ELISA kit available for autophagy research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-212-0001 96 wells 510.00 USD
 

Grp78/BiP ELISA kit 

High-sensitivity ELISA kit for quantifying both normal and upregulated levels of Grp78/BiP for unfolded protein response, cancer and neurodegenerative disease research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-214-0001 96 wells 510.00 USD
 

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